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blue collar underworld Reged: 07/19/13 Posts: 22008 Loc: sub-surface unse |
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question: if these plates are "poured" in larger quantities (say 3 TBS instead of 1 TBS per plate) would they make for longer term storage in the fridge for cultures because of the increased depth (wrapped in plastic/bagged up of course)? I do not have $ for slants right now, or will I in the next 2 months. I have some untested cube isolates, as well as maitaki and skiitaki clones taken from grocery store that grew out clean, all of them r on these pasty plates, and time restrictions r forcing me to delay the starts of newer grow projects. how long do u think these could last in fridge with a larger amount of agar...and would it be beneficial to tilt them a bit, like with a slant, while cooling if this was to be done? I have had this happen inadvertently while they cooled in the pc, and those plates are a bitch to transfer from because the agar is so deep in the middle of the culture, but it got me thinking about the relationship to the agars depth and its shelf life. Im going to have to do it either way but I wanted to throw it out there for opinions. also might as well ask...what is the longest any of you has kept a petri/easy plate/no pour plate WITH growth in the fridge and it still remain viable? I personally have used colonized grain jars after 5 months with success, but have never used (or tried) agar from fridge that was longer than a month. (Pasty, I can move this to a new thread if u want, its not meant to thread jack, but I put it here because ur plates can be poured to a larger depth than petris, so i thought it was related enough to make post here, and if it works, u can add the info @ the end of ur tek? )
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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