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ShroomKitty
The Curious Cultivating Cat

Registered: 01/28/09
Posts: 379
Loc: The Second Bardo
Last seen: 10 years, 3 months
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Ps. Azurescens Germination Q
#9694139 - 01/28/09 01:21 PM (15 years, 2 days ago) |
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Greetings all,
I’m glad to be here.
I’m trying for an outdoor Psilocybe Azurescens patch. 5 days ago I inoculated quart rye jars with about 3CCs from a syringe and shook. Temp at 80 F. Since I have well water that may be high in iron I used store bought spring water. (If that’s no good I’ll use distilled)
So far I have ZERO activity so I gave another shake. Should I see activity by now or am I jumping the gun? I plan on inoculating 3 more jars in a day or two if I messed up somewhere.
When should Ps. Azur start to germinate?
-------------------- I only cultivate legal mushrooms. All posts/pictures stating otherwise are fictional and are for entertainment purposes only. What my cat does in the alley is his business.
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Shr00mZ
Space Invader


Registered: 04/15/08
Posts: 1,454
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694146 - 01/28/09 01:22 PM (15 years, 2 days ago) |
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spore germination takes 3-15 days... you shaking it disturbes the spores... therefore its going to take longer to germinate. wait n see...
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Jitsu
JKD Love



Registered: 11/12/08
Posts: 1,073
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Re: Ps. Azurescens Germination Q [Re: Shr00mZ]
#9694189 - 01/28/09 01:33 PM (15 years, 2 days ago) |
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is it MS? Don't shake!
I am assuming it's MS since you are talking about Germination.
Germination of spores is often random but I have seen great germination within 3 days. Average I would say is anywhere from 5-8 days @ 73-82F
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
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Shr00mZ
Space Invader


Registered: 04/15/08
Posts: 1,454
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Re: Ps. Azurescens Germination Q [Re: Jitsu]
#9694213 - 01/28/09 01:38 PM (15 years, 2 days ago) |
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of course its MS... if its going to germinate.. Average doesnt mean anything. 3-15 days. you want it at room temp. 80F or higher is too warm.
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ShroomKitty
The Curious Cultivating Cat

Registered: 01/28/09
Posts: 379
Loc: The Second Bardo
Last seen: 10 years, 3 months
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Re: Ps. Azurescens Germination Q [Re: Shr00mZ]
#9694252 - 01/28/09 01:48 PM (15 years, 2 days ago) |
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It’s a multi spore syringe.
I thought you were suppose to shake the jar.
-------------------- I only cultivate legal mushrooms. All posts/pictures stating otherwise are fictional and are for entertainment purposes only. What my cat does in the alley is his business.
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German Kahuna
Facepalmer of Stoopid



Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: Ps. Azurescens Germination Q [Re: Jitsu]
#9694263 - 01/28/09 01:50 PM (15 years, 2 days ago) |
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Ok, where to start... You are going at it the wrong way (you won't necessarily fail, but you could have taken a few extra steps to assure success). If it's azurescens, the spores in your syringe will in all likelihood have come from an outdoor patch as well. They will also be rich in contaminants. See one of my current projects:
This is before cleaning up the culture (see the red circled area. It's the only one in there with clean P. azurescens mycelium, the rest is full of nasty bacterial growth):

You then cut wedges out of the clean mycelium spot and transfer them onto a new dish. Five days later:

So there. Clean mycelium. That was actually the second transfer after inoculating the first dish from a wild spore print.
It's now in a jar on a shelf awaiting colonization of the rye and then being transferred to the actual primary spawn which will be sterilized beech shavings in bags.
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MushHunter08
Mycological Pupil


Registered: 06/08/08
Posts: 522
Loc: B.F.E
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694266 - 01/28/09 01:51 PM (15 years, 2 days ago) |
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You only start shaking the jar after the grain is 30% colonized or a little better w/ mycelium. Not while its still in its multispore solution state.
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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Jitsu
JKD Love



Registered: 11/12/08
Posts: 1,073
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694269 - 01/28/09 01:51 PM (15 years, 2 days ago) |
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Quote:
Ta2dave said: It’s a multi spore syringe.
I thought you were suppose to shake the jar.
Shake after it's germinated. Usually those who shake immediately after inoculation are using a myceliem syringe.
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
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ShroomKitty
The Curious Cultivating Cat

Registered: 01/28/09
Posts: 379
Loc: The Second Bardo
Last seen: 10 years, 3 months
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Re: Ps. Azurescens Germination Q [Re: MushHunter08]
#9694276 - 01/28/09 01:53 PM (15 years, 2 days ago) |
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I used one of the “New Moon” spore syringes from Earth‘s Tongue.
-------------------- I only cultivate legal mushrooms. All posts/pictures stating otherwise are fictional and are for entertainment purposes only. What my cat does in the alley is his business.
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German Kahuna
Facepalmer of Stoopid



Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694288 - 01/28/09 01:58 PM (15 years, 2 days ago) |
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Oh, btw, 80°F is way too hot for P. azurescens! It's NOT a tropical species. Lower that by 10°-15°F at least. It will do fine at 65°-70°. 80°F is almost too high even for cubensis.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Quote:
German Kahuna said: Ok, where to start... You are going at it the wrong way (you won't necessarily fail, but you could have taken a few extra steps to assure success). If it's azurescens, the spores in your syringe will in all likelihood have come from an outdoor patch as well. They will also be rich in contaminants. .
They Might have contaminents. I know that some suppliers will not make syringes with outdoor mushrooms because of this reason. I have used many syrings made from prints that were taken from the wild and they have been fine. If the spore supplier was confident enough to sell a syringe then use it. I think you have better then 50/50 chance of it working.
Quote:
Shr00mZ said: spore germination takes 3-15 days... you shaking it disturbes the spores... therefore its going to take longer to germinate. wait n see...
Shaking has nothing to do with the length of time for spores to germinate.
Quote:
Jitsu said: is it MS? Don't shake!
I am assuming it's MS since you are talking about Germination.
Germination of spores is often random but I have seen great germination within 3 days. Average I would say is anywhere from 5-8 days @ 73-82F
He says dont shake with a !!!! Wtf? You can shake multi spore and you probably should. I worry about you saying that you have seen spores in three days. Some people will read this and think that they should see it in three days.
As for the original poster, give it at least two weeks before you start worrying
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Quote:
German Kahuna said: Oh, btw, 80°F is way too hot for P. azurescens! It's NOT a tropical species. Lower that by 10°-15°F at least. It will do fine at 65°-70°. 80°F is almost too high even for cubensis.
oh btw, it doesn't matter. Your explanation of why its too warm is silly. You can do all your pre fruiting work in the same temp ranges as cubes.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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German Kahuna
Facepalmer of Stoopid



Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: Ps. Azurescens Germination Q [Re: tahoe]
#9694358 - 01/28/09 02:11 PM (15 years, 2 days ago) |
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Quote:
tahoe said: They Might have contaminents. I know that some suppliers will not make syringes with outdoor mushrooms because of this reason. I have used many syrings made from prints that were taken from the wild and they have been fine. If the spore supplier was confident enough to sell a syringe then use it. I think you have better then 50/50 chance of it working.
Yeah, but why take the risk? I know for a FACT that my cultures are clean after I do the isolation work on agar.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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German Kahuna
Facepalmer of Stoopid



Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: Ps. Azurescens Germination Q [Re: tahoe]
#9694364 - 01/28/09 02:12 PM (15 years, 2 days ago) |
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Quote:
tahoe said:
Quote:
German Kahuna said: Oh, btw, 80°F is way too hot for P. azurescens! It's NOT a tropical species. Lower that by 10°-15°F at least. It will do fine at 65°-70°. 80°F is almost too high even for cubensis.
oh btw, it doesn't matter. Your explanation of why its too warm is silly. You can do all your pre fruiting work in the same temp ranges as cubes.
I stand corrected then. 80° is still a bit on the warm side.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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ShroomKitty
The Curious Cultivating Cat

Registered: 01/28/09
Posts: 379
Loc: The Second Bardo
Last seen: 10 years, 3 months
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I just looked at Paul Stamets book Mushroom Cultivator that spawn temp for cubies is 82-86F and Ps. cyanescens (closest I could find to Azur) was 65-75F.
So is 65-75F good for Azur of 80F?
-------------------- I only cultivate legal mushrooms. All posts/pictures stating otherwise are fictional and are for entertainment purposes only. What my cat does in the alley is his business.
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German Kahuna
Facepalmer of Stoopid



Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694462 - 01/28/09 02:29 PM (15 years, 2 days ago) |
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Quote:
I just looked at Paul Stamets book Mushroom Cultivator that spawn temp for cubies is 82-86F and Ps. cyanescens (closest I could find to Azur) was 65-75F.
So is 65-75F good for Azur of 80F?
How would I know? I am just a stupid idiot talking bullshit all day long. Ask tahoe.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
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Re: Ps. Azurescens Germination Q [Re: ShroomKitty]
#9694468 - 01/28/09 02:31 PM (15 years, 2 days ago) |
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Sorry to high jack your thread ta2, but I have a question in this same realm as well and I don't want to clutter the board with similar questions:
Would this method of inoculation work for woodlovers -
1.) Soak little pieces of cut up card board and put a thin layer on the bottom of jar. 2.) Inoculate with syringe/print. 3.) Once colonized, fill the jar with moistened wood chips.
It sounds like it would work, but I've never done woodlovers before, so I'm not sure. I know that you are able to be much less sterile, since the wood/cardboard is not colonized by molds as easily as grains. I've never done agar before either, and am trying to hold off until I have a more sterile environment and a pressure cooker.
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
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Re: Ps. Azurescens Germination Q [Re: libertaire]
#9694966 - 01/28/09 03:45 PM (15 years, 2 days ago) |
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Any thoughts?
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mister
Nature nut



Registered: 10/04/08
Posts: 2,926
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Re: Ps. Azurescens Germination Q [Re: libertaire]
#9695031 - 01/28/09 03:56 PM (15 years, 2 days ago) |
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Take them out of the heat and just give them room temp. Azure spores have taken as long as 2 weeks to germinate for me. They are very different than cubes. Do not expect to see rhizos from them like you would a cube. Also that quart can take you up to 1 1/2 to 2 months to colonize.
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mister
Nature nut



Registered: 10/04/08
Posts: 2,926
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Re: Ps. Azurescens Germination Q [Re: mister]
#9695062 - 01/28/09 04:00 PM (15 years, 2 days ago) |
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Note this statement. You bought a new moon syringe, this means you got hosed. Get one from sporeworks. That Earths Tounge shit is shit. Been there, done that, never will again.
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