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Offlinethemycologist
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Registered: 02/28/07
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Last seen: 12 years, 11 months
boil turbinado lc?
    #7551866 - 10/23/07 05:17 PM (16 years, 3 months ago)

planning to add 3 packets of "sugar in the raw" to a quart jar filled with 660ml of hot bottled water. then put that in a pot with a few inches of boiling water, cover, and boil for 30 min.

will this sterilize it well enough?

having trouble with my pc (see here).

had enough failures with honey.

thanks!


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


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InvisibleThePyschonaut52
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Re: boil turbinado lc? [Re: themycologist]
    #7551873 - 10/23/07 05:21 PM (16 years, 3 months ago)

boil the water for at least ten minutes first. then use that water to make the LC and boil for 30 minutes


--------------------
"In god we trust..."


-I guess we're screwed.


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Offlinethemycologist
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Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: ThePyschonaut52]
    #7555246 - 10/24/07 02:41 PM (16 years, 3 months ago)

ok, good deal.

also, reading some contradicting info on when to start, and how often to use a magnetic stirrer.

is the consensus that, when using spores, wait a few days for them to germinate, then use magnetic stirrer on low, 24/7?

thanks!


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: themycologist]
    #7583612 - 11/01/07 11:07 AM (16 years, 2 months ago)

lc was prepared as stated above (3 tsps sugar in the raw, 600ml water).

knocked up with 2 cc's of spore solution (pf rs) 6 days ago.

lc looks the same as it did when inoculated. clear lc with just a golden tint to it, no sediment. jar has been sitting idle at around 70 degrees.

do the temps need to be upped?
should it go on the magnetic stirrer, 24/7? was waiting for germination before doing this, but it seems it should have happened by now??

thanks!


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


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InvisibleSlimz
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Re: boil turbinado lc? [Re: themycologist]
    #7583627 - 11/01/07 11:10 AM (16 years, 2 months ago)

how long.. should see growth within a few days...


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Offlinethemycologist
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Re: boil turbinado lc? [Re: themycologist]
    #7583677 - 11/01/07 11:23 AM (16 years, 2 months ago)

Quote:

themycologist said:

knocked up with 2 cc's of spore solution (pf rs) 6 days ago.





should conditions be changed? was enough sugar used?


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Edited by themycologist (11/01/07 11:30 AM)


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Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: themycologist]
    #7592337 - 11/03/07 08:00 PM (16 years, 2 months ago)

anyone?

agar, any insight?

thanks!


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
Offlineacommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi


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Re: boil turbinado lc? *DELETED* [Re: themycologist]
    #7592451 - 11/03/07 08:33 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



--------------------


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Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: acommunistspy]
    #7592620 - 11/03/07 09:21 PM (16 years, 2 months ago)

Quote:

acommunistspy said:
also, do you by any chance see tiny black dots on the bottom of the jar? because i do and i used 2cc and i'm hoping its spores (my syringe was dark as shit).




yes, i see this also - a few clumps of spores were visible when first inoculated and looked unchanged after 6 days (couple tiny clumps of black dots). hopefully they will germinate soon - temp has been raised to around 76.


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
Offlineacommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi


Folding@home Statistics
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 13 years, 10 months
Re: boil turbinado lc? *DELETED* [Re: themycologist]
    #7594718 - 11/04/07 02:03 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



--------------------


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Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: acommunistspy]
    #7598571 - 11/05/07 01:40 PM (16 years, 2 months ago)

Quote:

acommunistspy said:
i've read that only 5 out of 100 spores germinate. that's why shrooms produce so many spores.




interesting...

gonna check it again in a few days - hopefully raising the temp a little helped.


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: themycologist]
    #7615913 - 11/09/07 04:01 PM (16 years, 2 months ago)

well, it still looks the same. clear with a slightly golden tint. no contams visible, but neither is anything else? been almost 2 weeks now, temp around 70. on the magnetic stirrer now.

why didn't this work?


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
Invisiblefastfred
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Re: boil turbinado lc? [Re: themycologist]
    #7616488 - 11/09/07 07:15 PM (16 years, 2 months ago)

You could have saved some time if you'd researched this.

Myc will only grow in more complex sugars. Dextrose, maltose, and to some extent fructose.

You're trying to use sucrose, which is going to work piss poor if at all.


-FF


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Offlinethemycologist
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Registered: 02/28/07
Posts: 205
Loc: spiritual enlightenment
Last seen: 12 years, 11 months
Re: boil turbinado lc? [Re: fastfred]
    #7620244 - 11/10/07 08:34 PM (16 years, 2 months ago)

Quote:

fastfred said:
You could have saved some time if you'd researched this.

Myc will only grow in more complex sugars. Dextrose, maltose, and to some extent fructose.

You're trying to use sucrose, which is going to work piss poor if at all.


-FF




reseacrch was done. this tek has been corroborated by several (here). the only difference was that a pc wasn't used, however, with the way everything was boiled and sterilized this shouldn't have made a difference.

this isn't simply adding white sugar to water.


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*


Extras: Filter Print Post Top
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