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OfflineSkizor1337
Trip King
I'm a teapot User Gallery


Registered: 06/19/12
Posts: 1,106
Loc: USA Flag
Last seen: 2 days, 19 hours
Healthy or Unhealthy Spawn?
    #28590307 - 12/19/23 06:48 PM (1 month, 8 days ago)

I have 2qt jars I'd like to throw into a shoebox here soon inoculated on LC that was tested on agar prior.

Good or bad?
The first four pics are jar one and the last four pics are jar two



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100% Professional Psychonaut
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OfflineRoscoeReturnsS
Crotchety chode man
Male


Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 8 hours, 3 minutes
Re: Healthy or Unhealthy Spawn? [Re: Skizor1337]
    #28590318 - 12/19/23 06:58 PM (1 month, 8 days ago)

Bacterial, but if you wait for them to finish up they will get you something in a shoebox. I’ve spawned worse.


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OfflineSkizor1337
Trip King
I'm a teapot User Gallery


Registered: 06/19/12
Posts: 1,106
Loc: USA Flag
Last seen: 2 days, 19 hours
Re: Healthy or Unhealthy Spawn? [Re: RoscoeReturns]
    #28590326 - 12/19/23 07:05 PM (1 month, 8 days ago)

Quote:

RoscoeReturns said:
Bacterial, but if you wait for them to finish up they will get you something in a shoebox. I’ve spawned worse.




Mmmmm damn lol - I'll probably spawn them anyway but how does it typically get bacterial?


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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Healthy or Unhealthy Spawn? [Re: Skizor1337]
    #28590347 - 12/19/23 07:17 PM (1 month, 8 days ago)

Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


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OfflineSkizor1337
Trip King
I'm a teapot User Gallery


Registered: 06/19/12
Posts: 1,106
Loc: USA Flag
Last seen: 2 days, 19 hours
Re: Healthy or Unhealthy Spawn? [Re: Way]
    #28590373 - 12/19/23 07:29 PM (1 month, 8 days ago)

Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi


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100% Professional Psychonaut
Trippin' Aint Easy




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Invisiblestarvinghooker
Voyager
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Registered: 04/16/23
Posts: 533
Re: Healthy or Unhealthy Spawn? [Re: Skizor1337]
    #28590528 - 12/19/23 09:13 PM (1 month, 8 days ago)

Quote:

Skizor1337 said:
Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi




Agar is definitely the right plan.  I'm not sure what the current thoughts on G2G are, but it can be tricky, even when you have clean spawn.

Do you vent your PC first?  Folks here lead me to venting and it really did help.


--------------------
Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons

Noob like me? Start here!
Hitchhiker's Guide to the Shroomery



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OfflineKnowlesKnows
Stranger

Registered: 10/29/23
Posts: 68
Last seen: 28 days, 22 hours
Re: Healthy or Unhealthy Spawn? [Re: Skizor1337]
    #28590737 - 12/20/23 01:22 AM (1 month, 8 days ago)

Quote:

Skizor1337 said:
Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi




I’ve tried LC twice. One made by myself and one made by a friend - both went green in 4-5 days whereas with agar transfers everything goes lovely every single time.

You need to be a wizard to get LC working correctly in my experience!


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OfflineSkizor1337
Trip King
I'm a teapot User Gallery


Registered: 06/19/12
Posts: 1,106
Loc: USA Flag
Last seen: 2 days, 19 hours
Re: Healthy or Unhealthy Spawn? [Re: starvinghooker]
    #28590915 - 12/20/23 06:59 AM (1 month, 7 days ago)

Quote:

starvinghooker said:
Quote:

Skizor1337 said:
Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi




Agar is definitely the right plan.  I'm not sure what the current thoughts on G2G are, but it can be tricky, even when you have clean spawn.

Do you vent your PC first?  Folks here lead me to venting and it really did help.




Yes I have the rocker off and let it steam out for at least a solid 5 minutes before putting the rocker back on


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100% Professional Psychonaut
Trippin' Aint Easy




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OfflineRockinRobot
Stranger


Registered: 12/08/22
Posts: 861
Last seen: 9 hours, 24 minutes
Re: Healthy or Unhealthy Spawn? [Re: Skizor1337]
    #28590921 - 12/20/23 07:05 AM (1 month, 7 days ago)

Quote:

Skizor1337 said:
Quote:

starvinghooker said:
Quote:

Skizor1337 said:
Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi




Agar is definitely the right plan.  I'm not sure what the current thoughts on G2G are, but it can be tricky, even when you have clean spawn.

Do you vent your PC first?  Folks here lead me to venting and it really did help.




Yes I have the rocker off and let it steam out for at least a solid 5 minutes before putting the rocker back on




Let it vent for at least 10 minutes, preferably 15.

I also go a full 2 hours on grain jars, but I'm a little anal. I figure the extra 1/2 hour is worth it.

I'm guessing you both made your LC by shooting spores into LC broth and that's probably why it all went green fast. Only good way to make LC is to grow on agar till you sure you have clean culture then make the LC from that.


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Invisiblestarvinghooker
Voyager
 User Gallery


Registered: 04/16/23
Posts: 533
Re: Healthy or Unhealthy Spawn? [Re: RockinRobot]
    #28590960 - 12/20/23 07:36 AM (1 month, 7 days ago)

Same here.  2hrs every time.  Makes me feel better lol.


--------------------
Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons

Noob like me? Start here!
Hitchhiker's Guide to the Shroomery



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OfflineRockinRobot
Stranger


Registered: 12/08/22
Posts: 861
Last seen: 9 hours, 24 minutes
Re: Healthy or Unhealthy Spawn? [Re: KnowlesKnows]
    #28590964 - 12/20/23 07:40 AM (1 month, 7 days ago)

Quote:

KnowlesKnows said:
Quote:

Skizor1337 said:
Quote:

Way said:
Either the LC wasn't as clean as you think, the grains were improperly sterilized, or your sterile technique needs work.




I think Im going to just start using Agar to grains to remove that step entirely and then do some G2G transfers.

I PC for 1hr 45mins on 15psi




I’ve tried LC twice. One made by myself and one made by a friend - both went green in 4-5 days whereas with agar transfers everything goes lovely every single time.

You need to be a wizard to get LC working correctly in my experience!




You don't need to be a wizard to make LC. You DO need to be good with agar and cleaning up cultures though. You can't make good LC from spores or bad cultures.

Once you get good at getting clean cultures on agar you can use this TEK to reliably get good LC. LC still needs to be tested once finished but has worked well for me.

https://www.shroomery.org/forums/showflat.php/Number/24740168


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