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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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OfflineShrooma doom
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Dead liquid culture
    #28547871 - 11/18/23 12:08 PM (2 months, 8 days ago)

I used some liquid culture the other day on grain and it’s showing no sign of mycelium growth so I’m assuming it was dead. Is that guaranteed contamination in the jars now? I hate wasting grain like that is there any chance you could inoculate them with another liquid culture and it work with no contamination?


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Offline10ftTall
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Re: Dead liquid culture [Re: Shrooma doom]
    #28547963 - 11/18/23 02:03 PM (2 months, 8 days ago)

LC to grain is not the way to go.


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InvisibleWay
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Re: Dead liquid culture [Re: 10ftTall]
    #28547975 - 11/18/23 02:10 PM (2 months, 8 days ago)

How long has it been?

Take the LC and test it on agar to see what you get. You shouldn't ever use LC without knowing if it is clean or not.

LC is a lot harder to replace than some grains that you sent through the PC. I wouldn't just go shooting more in there personally. Of course there is a chance that it works out, but if the LC was actually good and the grain isn't colonizing for some other reason, you're wasting more LC.


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OfflineKinoko314
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Re: Dead liquid culture [Re: Way]
    #28548035 - 11/18/23 02:53 PM (2 months, 8 days ago)

It's likely that you just need to wait longer.  It can take some time to get going.


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OfflineBlueberry Muffin
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Re: Dead liquid culture [Re: Kinoko314]
    #28548044 - 11/18/23 02:57 PM (2 months, 8 days ago)

You didn't mention how long you had the lc for and what formula you used


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OfflineShrooma doom
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Re: Dead liquid culture [Re: Blueberry Muffin]
    #28548126 - 11/18/23 03:48 PM (2 months, 8 days ago)

It was a syringe I got from a itw a few months ago. I inoculated the jars about a week ago. I noticed it was gelled up around the plunger too but I don’t see any contamination in the jar yet.


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OfflineKinoko314
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Re: Dead liquid culture [Re: Shrooma doom]
    #28548130 - 11/18/23 03:55 PM (2 months, 8 days ago)

I'd say it should show growth by 10 days for sure.  Sometimes it seems to take a while to start and then there's suddenly growth everywhere.  That could still happen.  I guess you don't know exactly what day you inoculated, so maybe approximate two weeks instead of 10 days before you just throw it out.

I wouldn't re-inoculate the same jars.  If you can just prep some new ones that would be much better.


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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures


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