|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
Swabs to agar
#27978165 - 10/02/22 02:56 PM (1 year, 3 months ago) |
|
|
If you had 6 swabs gifted to you months back, 3 varieties, and you wanted to make agar plates today and swab them with your swabs, how many would you make?
Can you make 20 plates with that?
Assume you tried pulling off a old print and got nothing out of 20 plates last attempt
-------------------- Within You , Without You
      
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 7 hours, 12 minutes
|
Re: Swabs to agar [Re: Enkidu]
#27978201 - 10/02/22 03:29 PM (1 year, 3 months ago) |
|
|
So many numbers lol I suppose seems how it's 3 varieties I'd swab 3 plates, save 3 swabs to try incase they dont take.
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
Re: Swabs to agar [Re: rumfor69]
#27978223 - 10/02/22 03:42 PM (1 year, 3 months ago) |
|
|
Only 3?
You can't or shouldn't stretch them out to try and hit multiple plates?
-------------------- Within You , Without You
      
|
Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 13 hours
|
Re: Swabs to agar [Re: Enkidu]
#27978231 - 10/02/22 03:48 PM (1 year, 3 months ago) |
|
|
Here is an easy way to do 2 plates per swab:
Cut the tip off. Stuff it in a plate. Use the rest to swab another plate
(I would also use only 3 like Rumfor said and save 3 for later in case - that magician is a wise one)
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
|
Jaksavage
Mycodelic



Registered: 11/19/21
Posts: 548
Loc: Left coast
Last seen: 5 months, 29 days
|
Re: Swabs to agar [Re: Enkidu]
#27978235 - 10/02/22 03:50 PM (1 year, 3 months ago) |
|
|
I'd start 3 plates of each variety, giving you 9 starts.
--------------------
The Shroomery is the biggest library with the coolest librarians
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 7 hours, 12 minutes
|
Re: Swabs to agar [Re: Enkidu]
#27978236 - 10/02/22 03:51 PM (1 year, 3 months ago) |
|
|
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
Re: Swabs to agar [Re: rumfor69]
#27978250 - 10/02/22 04:04 PM (1 year, 3 months ago) |
|
|
So like take one of each swab, cut out a bit and plant it in a plate, swab another plate with the leftover, make 6-9 plates from 3 swabs total, save 3 swabs for later ?
-------------------- Within You , Without You
      
|
Jaksavage
Mycodelic



Registered: 11/19/21
Posts: 548
Loc: Left coast
Last seen: 5 months, 29 days
|
Re: Swabs to agar [Re: Enkidu] 1
#27978254 - 10/02/22 04:07 PM (1 year, 3 months ago) |
|
|
Good plan. Wet your swab with sterile h2o before you swab plate
--------------------
The Shroomery is the biggest library with the coolest librarians
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
|
So sterilize some water while I pc my agar?
I guess I'll just make 20 plates and then store the rest in the fridge
-------------------- Within You , Without You
      
|
Jaksavage
Mycodelic



Registered: 11/19/21
Posts: 548
Loc: Left coast
Last seen: 5 months, 29 days
|
Re: Swabs to agar [Re: Enkidu]
#27978273 - 10/02/22 04:15 PM (1 year, 3 months ago) |
|
|
Yes and yes.
--------------------
The Shroomery is the biggest library with the coolest librarians
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 7 hours, 12 minutes
|
|
Steril water can help for sure. I'm lazy so I do that as a last resort with the backup swabs lol
You can always just suran wrap extra plates and save for later like ya say. You'll need em for transfers anyhow with all the success you're about to have!
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
|
Appreciate the good words and thank you fellers for all the help
-------------------- Within You , Without You
      
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
Re: Swabs to agar [Re: Enkidu]
#27978326 - 10/02/22 04:44 PM (1 year, 3 months ago) |
|
|
My apologies, I have more
I use to do 10g dme and 10g agar in 500ml water, will that work or do you need to alter your ratio for any reason?
-------------------- Within You , Without You
      
|
Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 13 hours
|
Re: Swabs to agar [Re: Enkidu]
#27978332 - 10/02/22 04:48 PM (1 year, 3 months ago) |
|
|
It'll work.
Most people use LME and not DME.
Some people like softer plates for germination (maybe 7g agar/500ml) - I don't care about the softer plates as I use a loop and it does deposit water on my agar
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
|
Thanks man, I'll have to get some lme to try
-------------------- Within You , Without You
      
|
Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 13 hours
|
Re: Swabs to agar [Re: Enkidu]
#27978351 - 10/02/22 04:57 PM (1 year, 3 months ago) |
|
|
Quote:
Enkidu said: Thanks man, I'll have to get some lme to try 
Or just use grain water agar (GWA)
Cheap but most of all very accessible and no delivery time 
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
Re: Swabs to agar [Re: Enkidu]
#27978356 - 10/02/22 05:00 PM (1 year, 3 months ago) |
|
|
Grain water huh ? I'll have to look that up once I start prepping grain it sounds like
-------------------- Within You , Without You
      
|
Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 13 hours
|
Re: Swabs to agar [Re: Enkidu]
#27978358 - 10/02/22 05:03 PM (1 year, 3 months ago) |
|
|
You don't even need to look it up.
Strain your grain water after cooking the grain.
Strain again through a coffee filter. Mix 500ml GW with 9g agar (or whatever quantity is your favorite) and PC for 15-30 min - I do 15 but most people here seem to think 30 is safer.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
|
Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,698
Last seen: 6 months, 10 days
|
|
Awesome, thanks man I'll give it a shot once I get to that point
I have some prints too it looks like, I'll try hydrating them with some of this water and transfer to some agar and see if I can get any of those going
-------------------- Within You , Without You
      
|
Jaksavage
Mycodelic



Registered: 11/19/21
Posts: 548
Loc: Left coast
Last seen: 5 months, 29 days
|
Re: Swabs to agar [Re: Enkidu]
#27978450 - 10/02/22 06:14 PM (1 year, 3 months ago) |
|
|
Hydrate your swabs before swiping them. For a print, use a loop or a flame sterile knife to scrape some spore print.
IMO
good luck
--------------------
The Shroomery is the biggest library with the coolest librarians
|
|