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Offlinekherodan
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New to agar, wondering if I have Trich or healthy mycelium. (pic included)
    #27707887 - 03/25/22 08:59 AM (1 year, 9 months ago)





First pic is of transfer 1 and second is of transfer 2. In transfer 1 you can see the transfer agar in the middle. Seemed like mycelium sprouted off from there in the biggining but I noticed some really dense bright white growth off to the side (where the square of Transfer 2 is located, on the right), I thought I might have scraped some of the transfer there when doing my original transfer so I thought it was probably not contamination. The stuff sprouting from the middle waned in whiteness while the dense white growth stays the same after about 10 days.

Now, the second pic contains transfer 2, and this is growth 3 days after the transfer. I'm wondering if it's healthy cube mycelium or Trichoderma.

Thanks for the help.


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InvisibleGoatrider
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27707930 - 03/25/22 09:54 AM (1 year, 9 months ago)

You don´t want cube myc growing like that 3 days after transfer.
First plate seems to be pretty pin mold.
Me personally would never transfer from a pin mold plate,
still possible though.
How´s the germination plate looking?
There may be a chance on another transfer at 7-8 o´clock from the first plate near the edge.
In the meantime just shoot up new plates :wink:


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Offlinekherodan
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: Goatrider]
    #27707943 - 03/25/22 10:10 AM (1 year, 9 months ago)

Quote:

Goatrider said:
You don´t want cube myc growing like that 3 days after transfer.




You mean it's growing too quickly and/or looks too fluffy? Should I toss it before it gets to my other plates or should I wait it out to see? Is there no way it could be healthy mycelium? It has turned green yet....

Quote:

Goatrider said:
How´s the germination plate looking?




This is what it looks like.



I used Malabar spore prints. You can see there's another section I cut out and transferred (transfer B). Transfer B seemed to have pretty healthy uniform mycelium which I then transfered 2 days ago to three new plates. Those plates are progressing at a slower rate. I imagine these should be good will post up in a day or two.


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OfflineDERRAYLD
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27707950 - 03/25/22 10:20 AM (1 year, 9 months ago)

That germ plate is filthy,  I would not even try unless you only have that single plate.

Start over and try to streak a small amount of spores rather than that abomination of a plate.

:douchewink:


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Offlinedyel
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: DERRAYLD]
    #27707974 - 03/25/22 10:48 AM (1 year, 9 months ago)

I have oysters that grow exactly like the second plate, maybe you got oysters contam i hear it happens a lot :lol:


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Offlinekherodan
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: dyel]
    #27707983 - 03/25/22 10:58 AM (1 year, 9 months ago)

Quote:

dyel said:
I have oysters that grow exactly like the second plate, maybe you got oysters contam i hear it happens a lot :lol:



Got my spore prints from a 'reputable vendor' so I really hope you're joking and they didn't send me oyster shrooms. Are you sure there's no way that's cube mycelium?

I'll show you folks my other plates, they seem to be growing a bit differently, I nocced them two days ago from a different transfer plate than the one in the OP.









can't it just be tomentose growth?


Edited by kherodan (03/25/22 11:00 AM)


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Offlinedyel
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27707993 - 03/25/22 11:03 AM (1 year, 9 months ago)

I don't think they sell oysters spore syringes anywhere, you can get them from the environment though
it can also be mold or cubes, just mentioned that i get similar oyster looking myc


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Offlinekherodan
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: dyel]
    #27707997 - 03/25/22 11:09 AM (1 year, 9 months ago)

Does cube mycelium look vastly different?


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InvisibleMTZ
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27708007 - 03/25/22 11:22 AM (1 year, 9 months ago)

That's cubensis. It's freaking out really. It has a lot going on. It's basically in a fight against the contamination.


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Offlinekherodan
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: MTZ]
    #27708020 - 03/25/22 11:35 AM (1 year, 9 months ago)

Which picture are you referring to? The second one in the original post?

Why do you say it appears to be fighting against contaminations, because it's trying to spread itself as far as possible?


Edited by kherodan (03/25/22 11:35 AM)


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Offlinedyel
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27708994 - 03/26/22 03:08 AM (1 year, 9 months ago)

relax dude, let it grow for a couple of days and see what happens.
also have anyone else noticed more tomamtose growth on pasty plates? lots of oxigen inside maybe?


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Offlinehazyhorse
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: dyel]
    #27709002 - 03/26/22 03:38 AM (1 year, 9 months ago)

i think tomentose growth arises a lot from different nutrient ratios. i’m sure there are plenty of reasons it can, & maybe air exchange is one of them, but i know for sure your nutrient ratios play a major factor. from what i understand, lower nutes will force the myc to hunt a bit more & it’ll act differently than if your agar is rich & it doesn’t have to go far.

oyster myc looks pretty different & is fast, aggressive, & in my experience very whispy. i’m gonna go out on a limb & venture to guess there is a 0% chance it’s an oyster myc contamination lol.

trich turns green almost immediately on agar IME, like even the tiniest dot on my plates will show color after a day. that germ plate does look rough but hey, might as well see how your new transfers go. it’s always hard to tell from pics w pasty plates/no pours but tomentose myc is completely fine & those plates don’t necessarily look horrible to me from what i can see. let it grow out for a few days & take a transfer. after a few you’ll be able to see how it’s performing


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Offlinedamp33
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: hazyhorse]
    #27709050 - 03/26/22 05:25 AM (1 year, 9 months ago)

Wait few days


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InvisibleMycoWeek
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: kherodan]
    #27709469 - 03/26/22 12:55 PM (1 year, 9 months ago)

Quote:

kherodan said:









I would streak a few new plate like others have already suggested, and go lighter this time. Meanwhile, just wait for 2nd plate to grow.

If you are really in doubt but still want to know if the 2nd plate culture is indeed mycelium, you can always just let it sit and see if it pins invitro. If it's mushroom culture, it will most likely form primordias and have few small mushrooms growing.

It can be frustrating running into issues like this, where contamination is hard to spot, but they are always valuable lessons.

if you need spores, PM me.


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Offlinedyel
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: hazyhorse]
    #27709473 - 03/26/22 12:59 PM (1 year, 9 months ago)

lol yeah I was joking around when I said it might be oyster contam, it's probably just tomatose growth
op should wait a few days and see what comes up


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InvisibleMycoWeek
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: dyel]
    #27709477 - 03/26/22 01:02 PM (1 year, 9 months ago)

Quote:

dyel said:
lol yeah I was joking around when I said it might be oyster contam, it's probably just tomatose growth
op should wait a few days and see what comes up




I have no idea how oyster mycelium looks like, but if OP let it grow out and pin invitro, it could be any kind of mushroom....so I guess it is possible we gonna see some oyster from this plate a month from now?


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Offlinedyel
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: MycoWeek]
    #27709482 - 03/26/22 01:09 PM (1 year, 9 months ago)

Not likely, i think this is just tomatose cube myc

but if you want to see how oyster plate pins look like here you go, im having a problem with premature pinning :lol:





Edited by dyel (03/26/22 01:29 PM)


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InvisiblePsychonauticsgod21
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: dyel]
    #27709569 - 03/26/22 02:35 PM (1 year, 9 months ago)

Probably is tomentose mycelium tbh I've had my GT plates all grow out kinda fluffy just like that, and Ive definitely seen it on different types of agar to it could be whatever ratio of mix you use for it!


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OnlineB Traven
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Re: New to agar, wondering if I have Trich or healthy mycelium. (pic included) [Re: Psychonauticsgod21]
    #27709624 - 03/26/22 03:30 PM (1 year, 9 months ago)

Yeah, as mentioned, I personally would decide what to do with that plate based on how many others I had. If I was long in plates, I'd toss it. If it was my only hope, I'd take it out to a few more transfers and watch closely.

If you got a whole syringe, definitely start a bunch more germ plates, for comparison if nothing else.


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