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Offlinewclifton1
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spore syringe quality?
    #27509505 - 10/18/21 08:31 PM (2 years, 3 months ago)

first, thanks to everyone on here for the amazing resources! first time grower, using agar tek from D3 and BOD, and planning to do Frank and BOD's mono tek using WBS once a i get the hang of agar!

ordered syringes from one of your sponsors, which i won't name yet, b/c this is prob my fault, not theirs. but also ordered some spore syringes of shiitake, lions mane, and blue oyster (for cooking!) and the content was so different i thought i would ask if I have bad spores to start with

first the B+ spore syringe from a sponsor:


next a blue oyster syringe:


just curious which is "normal"? the single clump of spores? or the murky swamp-like syringe?

here is my first two attempts at plating the B+... not sure if i actually got anything out of the syringe evey tho i shook it up a lot (it has been almost 3 weeks since i plated these) and not sure if this is mycelium or something vulgar... well one spot is obvious





Any feedback / help would be appreciated! Was pretty careful with my technique and used a SAB... but it was my first try.

Yesterday I ended up pushing all of the B+ syringe out into a sterile dish and grabbed the little black clumps with my flamed (and cooled) loop and smeared the suckers all over 3 new plates. will let you know how that goes...

THANK YOU SHROOMERY


Edited by wclifton1 (10/18/21 08:32 PM)


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OfflinePBJ710
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Re: spore syringe quality? [Re: wclifton1]
    #27509521 - 10/18/21 08:42 PM (2 years, 3 months ago)

The edibles are in liquid culture, the actives are spore syringes.  100% normal.

You wasted that syringe.  You only need a drop of MS solution on a loop to streak a plate.  Those black clumps are millions of spores all clustered together because you didn't shake the syringe well before use.  It only takes 2 spores to start a culture.


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Offlinewclifton1
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Re: spore syringe quality? [Re: PBJ710]
    #27509873 - 10/19/21 07:49 AM (2 years, 3 months ago)

thanks, i didn't realize the difference in liquid culture and spore syringes!

i did shake up the spore syringe each time, but the growth was so slow, i thought i was doing something wrong.

do the plates i posted look like they have some mycelium that i should transfer to new plates until i get something more rhizomorphic?


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OfflineHindsight
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Re: spore syringe quality? [Re: wclifton1]
    #27509879 - 10/19/21 07:57 AM (2 years, 3 months ago)

The top plate has mold at 11:00 and what looks like healthy mycelium at 1:00 growing in 3 different sectors. Bottom plate is too out of focus but it looks like I see a few sectors of healthy mycelium there. Both plates are ready to take transfers from.


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Offlinewclifton1
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Re: spore syringe quality? [Re: Hindsight]
    #27509896 - 10/19/21 08:22 AM (2 years, 3 months ago)

very much appreciate the quick and helpful response


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Invisiblesandman420
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Re: spore syringe quality? [Re: Hindsight]
    #27509899 - 10/19/21 08:23 AM (2 years, 3 months ago)

The top pic looks like the good growth has fuzzy mold around it and nothing to work with IMO.

The bottom plate isn't very clearly visible but it looks like a good plate to grab some transfers from.


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Offlinewclifton1
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Re: spore syringe quality? [Re: sandman420]
    #27509921 - 10/19/21 08:51 AM (2 years, 3 months ago)

thanks!! will stick w/ the bottom plate. hard to get good images with the condensation...


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Offlinewclifton1
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Re: spore syringe quality? [Re: wclifton1]
    #27528830 - 11/03/21 02:18 PM (2 years, 2 months ago)

UPDATES! here are my best two plates that i unloaded a whole spore syringe on... :rolleyes:

i went out of town and let these grow for too long, i know, i know...

blue plates source


red plates source


I transferred 6 wedges to 6 new plates... going to give it a few more days to grow out... but what do you all think should be my new "paterfamilias"??

blue1

blue2

blue3

red1

red2

red3


or do i suck at this too much?
pick a winner!
You may choose many


Votes accepted from (11/03/21 12:17 PM) to (No end specified)
You must vote before you can view the results of this poll



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Offlinewclifton1
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Re: spore syringe quality? [Re: wclifton1]
    #27529963 - 11/04/21 10:47 AM (2 years, 2 months ago)

thanks for the single vote  :icecreamsadness:


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OfflinePBJ710
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Re: spore syringe quality? [Re: wclifton1]
    #27530053 - 11/04/21 11:48 AM (2 years, 2 months ago)

They need more transfers to clean up the cultures before you can try to figure out which ones to keep.  I would have grabbed a transfer from 8 oclock on the blue plate source as the mycelium seems more aggressive there, but the others should work as well. 

Red 3 is a goner IMO.


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Offlinewclifton1
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Re: spore syringe quality? [Re: PBJ710]
    #27530580 - 11/04/21 08:35 PM (2 years, 2 months ago)

thanks PBJ, appreciate the input!

so i should prob do a couple more transfers before going to WBS jars?


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OfflinePBJ710
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Re: spore syringe quality? [Re: wclifton1]
    #27530586 - 11/04/21 08:44 PM (2 years, 2 months ago)

IMO yes - at least a T2 or T3 depending on how they grow out.  I don't like sending random T1 growth to grain since so much downstream depends on the health of that initial culture.


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Offlinewclifton1
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Re: spore syringe quality? [Re: PBJ710]
    #27556260 - 11/24/21 11:23 AM (2 years, 2 months ago)

woo hoo!
this 3rd generation of plates seems to be looking pretty healthy, no?
2nd row, bottom right is what i was thinking would be my master going forward. thoughts?

ready to spawn to grain?

growth has been pretty slow tho, this is 2.5 weeks in. i think it is b/c my house has been a little colder than usual due to the weather changes...


Edited by wclifton1 (11/24/21 11:26 AM)


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OfflineA.k.aM
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Re: spore syringe quality? [Re: wclifton1]
    #27556413 - 11/24/21 02:12 PM (2 years, 2 months ago)

Those dont look right, it looks like it might be the plates though. Either too soft or the myc doesn’t like the dye.


You should be getting solid growth similar to this.



The germ plates look normal, so it’s either technique or the new plates.


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Offlinewclifton1
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Re: spore syringe quality? [Re: A.k.a]
    #27556424 - 11/24/21 02:23 PM (2 years, 2 months ago)

ok well that's a bummer

maybe i'll go back to the previous generation and make new plates

these plates were made using D3's agar tek. but this latest set (purple) were made w a different batch of agar and different dye than the previous rounds (red and blue in the pics)

i'll just eliminate all dyes for now until i get the hang of it and go back to a previous generation

thanks!


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OfflineLieutenant Pan
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Re: spore syringe quality? [Re: wclifton1]
    #27556534 - 11/24/21 04:23 PM (2 years, 2 months ago)

AKA...that plate looks beautiful I tell ya h'wat


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Offlinewclifton1
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Re: spore syringe quality? [Re: Lieutenant Pan]
    #27573369 - 12/08/21 08:55 AM (2 years, 1 month ago)

ok, so i took your advice, threw out the funky P2 plates, made new agar without food coloring, and transferred from the healthy red P1 plates a generation ago

growth is looking a lot more uniform

also switched to glass dishes... no more condensation, love it!

not seeing that beautiful rhizo growth, but do these look ready for grain? or keep re-plating the best ones until i get rhizo?



Edited by wclifton1 (12/08/21 08:56 AM)


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Invisiblesandman420
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Re: spore syringe quality? [Re: wclifton1]
    #27573373 - 12/08/21 09:02 AM (2 years, 1 month ago)

What's your recipe?

You can add 1-2g of nutritional yeast and use less malt extract (12-15g/liter) to improve culture appearance.

Make sure you're not overcooking it, 15 minutes at 15psi is plenty.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (12/08/21 09:02 AM)


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Offlinewclifton1
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Re: spore syringe quality? [Re: sandman420]
    #27573492 - 12/08/21 10:44 AM (2 years, 1 month ago)

1L water
20g agar agar
15g LME

20min in Instant Pot High

i'll try some nutritional yeast next time


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Offlinewclifton1
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Re: spore syringe quality? [Re: wclifton1]
    #27574791 - 12/09/21 01:22 PM (2 years, 1 month ago)

anybody care to weigh in on if these are ready to spawn to grain? or would you do another transfer to see if we can get some rhizo growth?


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