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MushroomBush
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Registered: 01/20/19
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is this cube myc ?
#25782666 - 01/31/19 10:39 PM (4 years, 11 months ago) |
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did an experiment with a 5 year old cambodian print in a petri i found....made some agar the other day and had some extra so once it was almost cooled i said screw it and poured some agar over the print in the petri and stirred it all up with a q tip. I didnt think anything would happen because the print was so old but i think somthing might be goin on in there just want an opinion.
It would be awesome if it was cube myc because its from one of the first prints i ever took  
i believe it was from one of these three shrooms that grew out of an experiment i did in our goat pasture
Edited by MushroomBush (01/31/19 10:42 PM)
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somestupidnewbie
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Just to clarify.... you poured the hot agar over a spore print in a dish?
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MushroomBush
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yes...it was almost solidified so not hot enough to kill spores i dont think thats why it kinda looks clumpy
i was just fuckin around...didnt think anything would happen but if it turns out to be cube myc, then great
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Edited by MushroomBush (01/31/19 10:49 PM)
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somestupidnewbie
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Quote:
MushroomBush said: yes...it was almost solidified so not hot enough to kill spores i dont think thats why it kinda looks clumpy
i was just fuckin around...didnt think anything would happen but if it turns out to be cube myc, then great
Right on, I'm interested to see what happens
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MushroomBush
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me to, i thought about doing a transfer right away but i wanted to get an opinion first, i thought it might have turned green already if it was a contam
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Thesunbeam
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Prints last a lot longer than 5 years. Should have made some normal plates and inoculated like normal
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Caps McGee
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Quote:
Thesunbeam said: Prints last a lot longer than 5 years. Should have made some normal plates and inoculated like normal
But looks like cube myc... interested to see how getting a transfer goes...
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scarabaeus
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Registered: 06/13/18
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Definitely looks cubey. My attempts at old spore prints never took off -but that is just me. My cut-off age has always been ~3 years. But again, that could just be the way I store my prints or something else?
I've lost some precious prints to age, but from what I see (anyway), is that you are on yer way to success. Y R lookin' good so far IMO, good luck!
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MushroomBush
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i dont think it turned out to me cube, it turned a greenish color in the center
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scarabaeus
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Yeah, you are right on it -definitely not cube myc. All fungal contaminates start out white but then turn various colors when they mature a bit and start to sporulate. The most common contams that are greenish are aspergillus and penicillium species.
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SectorSelector
Selecting Sectors Since 2012



Registered: 01/31/19
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Loc: Dark Side of the Moon
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Quote:
MushroomBush said: i dont think it turned out to me cube, it turned a greenish color in the center

I'm more concerned about what your agar looks like.. 
What recipe did you use?
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scarabaeus
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You may have a point there sector. I just assumed the OP used food dye as some growers sometimes do. If not, something is very, very wrong lol!
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MushroomBush
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well it was from an outdoor print, and i poured it in open air. just fuckin around seeing if i could get anything from it
i used the standard 2% lme and a drop of blue food coloring
the agar looks clumpy like that because i waited until it was almost solidified in the clear beer bottle i use and shook it up and then poured it over top of the print in the petri and stirred it all up....let me make this clear thats not my usual procedure it was just an old ass print i had and i said fuck it im gonna try and get somthin out of it
I deffinately got somthing but not cubes lol
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SectorSelector
Selecting Sectors Since 2012



Registered: 01/31/19
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Loc: Dark Side of the Moon
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Quote:
MushroomBush said: well it was from an outdoor print, and i poured it in open air. just fuckin around seeing if i could get anything from it
i used the standard 2% lme and a drop of blue food coloring
the agar looks clumpy like that because i waited until it was almost solidified in the clear beer bottle i use and shook it up and then poured it over top of the print in the petri and stirred it all up....let me make this clear thats not my usual procedure it was just an old ass print i had and i said fuck it im gonna try and get somthin out of it
I deffinately got somthing but not cubes lol

Well... I respect your "fuck it" mentality lol you could of done this with sterile procedure and been successful. Ive cloned a bleached out mushroom that had been growing on the beach that was completely dried. Anything is possible with a little love and attention
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MushroomBush
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yea your absolutley right now that i think more on that.....it was just that i was done doing what i had planned to do and i had some extra agar in the bottle so i just kinda poured it in.
i have a few other prints from the same batch, there from 2016 tho ive never had luck with old spores thats why i didnt really expect anything to happen.
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MushroomBush
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"Psyilocybe Cambodian(MS) that was found in the back of the long term storage room. Never been used and formed very thick solid white mycelium. Never seen anything like this but we are going to fruit it out of curiosity. If anyone has ever seen anything like this please share! Several plates ended up with the same results."
this is a quote from your "check out my bulk grow" i acually have something similar going on at the moment
AA+ ms
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SectorSelector
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Registered: 01/31/19
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Quote:
MushroomBush said: "Psyilocybe Cambodian(MS) that was found in the back of the long term storage room. Never been used and formed very thick solid white mycelium. Never seen anything like this but we are going to fruit it out of curiosity. If anyone has ever seen anything like this please share! Several plates ended up with the same results."
this is a quote from your "check out my bulk grow" i acually have something similar going on at the moment
AA+ ms

'" its so strange! The mycelium is starting to pin. My lab partner decided to transfer "leading edge" we shall see what the fuck happens. As far as bringing back old spores try any Agar recipe with Activated Carbon. Thats suppose to help with old spores. I use MEPYAC. I have the recipe in the Bulk Grow link in my signature
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