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Offlinedrsquanch
MisterDoctorProfessor
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Registered: 11/07/18
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Growth on agar, not sure if mycelial. Microscope pics included.
    #25598508 - 11/07/18 12:34 PM (5 years, 2 months ago)

I have a PDA plate with some white growth on it that looks like mycelium to the naked eye but when I put it under my scope, I'm not completely sure. Here are some pics at 1000X mag.





As you can see, towards the bottom of the strand, there are long hyphae looking bodies, but upwards there are a lot of small cells that look like yeast forming pseudohyphae. I'm not sure if I should abandon hope or not because this was the one plate of like 8 that had anything resembling mycelium.
(edit)Also, here is a picture at 40X mag. I forgot to add this one when I first posted.


Edited by drsquanch (11/07/18 12:38 PM)


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Invisible36fuckin5
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Registered: 08/11/03
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: drsquanch]
    #25598946 - 11/07/18 03:33 PM (5 years, 2 months ago)

Show some normal pics of the plate. It seems fine from what I can see.

If in doubt, just wait. Contams will show up or not.


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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlinedrsquanch
MisterDoctorProfessor
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: 36fuckin5]
    #25600413 - 11/08/18 09:12 AM (5 years, 2 months ago)

Here are some normal pictures.




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Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: drsquanch]
    #25600515 - 11/08/18 09:45 AM (5 years, 2 months ago)

Lol let it grow out a little more. Very cool pictures though!


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

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EZEKIEL 23:20


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Offlinedrsquanch
MisterDoctorProfessor
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: elasticaltiger]
    #25600598 - 11/08/18 10:19 AM (5 years, 2 months ago)

Ugh. It’s so hard to wait. It’s been like a month since i knocked up these plates and there’s barely anything going on. I think i’m going to have to make a brf jar so i can get some mycelium going and transfer to agar.


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Invisibleelasticaltiger
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: drsquanch]
    #25600603 - 11/08/18 10:20 AM (5 years, 2 months ago)

That plate is a month old?  Time to start over man.  If my plates don't put out within a week or two I move on. Don't got time for that.

:drunkdriver:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinedrsquanch
MisterDoctorProfessor
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Registered: 11/07/18
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: elasticaltiger]
    #25600851 - 11/08/18 12:10 PM (5 years, 2 months ago)

Sadly yes. I actually made a new plate less than a week ago and knocked it up with some spores i got from drying a drop from a spire syringe on a microscope slide and there’s a small amount of bacterial contam but it seems like i can see a few of the spores germinating. Hopefully, they’ll mate and i’ll have a half decent colony soon.


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Invisibleelasticaltiger
Like Tigers in Coitus
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Posts: 8,042
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Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: drsquanch]
    #25600858 - 11/08/18 12:14 PM (5 years, 2 months ago)

Are you opening your dishes for the microscope viewings?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinedrsquanch
MisterDoctorProfessor
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Registered: 11/07/18
Posts: 5
Last seen: 4 years, 10 months
Re: Growth on agar, not sure if mycelial. Microscope pics included. [Re: elasticaltiger]
    #25601262 - 11/08/18 03:17 PM (5 years, 2 months ago)

I leave them closed for viewing at 40 and 100x but at 400 and 1000x the lenses hit the plastic, so if I want to see it in more detail I crack it open in my SAB and cut out pieces and prepare slides.


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