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Shop: Mushroom-Hut Liquid Cultures, Substrate Bags   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag

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Offlinedrink_me
One swell chap

Registered: 02/23/04
Posts: 8
Loc: UK
Last seen: 17 years, 5 months
Almost a month, nothing yet...
    #2478351 - 03/27/04 03:18 PM (19 years, 10 months ago)


I started my first attempt at the pf-tek on the 1st March, 6 jars with Matias Romero spores. I followed the pf-tek as best I could using a standard kitchen pan instead of a pressure cooker for sterilization. In the FAQ it says "Give it at least a week before you worry, and at least 2 weeks before you panic." So as you can imagine at close to 4 weeks I've just about given up hope! The jars are in the dark at approx 68 degrees and 52 humidity apparently. They look quite dry, any chance they're TOO dry?? I know this has probably been covered, my apologies if it has for my inability to find it on the boards here.

Any thoughts??


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InvisibleMESCALATO
hunting.
 User Gallery

Registered: 05/16/02
Posts: 1,025
Loc: Central Alabama
Re: Almost a month, nothing yet... [Re: drink_me]
    #2478360 - 03/27/04 03:24 PM (19 years, 10 months ago)

they need to be incubated at 82-85F. 68F is much to cold.


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OfflineReTrIbUtIoN
journeyman
Registered: 02/19/04
Posts: 80
Last seen: 19 years, 8 months
Re: Almost a month, nothing yet... [Re: drink_me]
    #2478388 - 03/27/04 03:40 PM (19 years, 10 months ago)

yes even at 68 degrees they woulda started by now. Like you said you prolly didnt use enough water and/or weak spore solution or you forgot to shake the syringe before hand. All possibilities


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"Don't drink and drive, You might spill your beer."


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OfflineErik006
MushroomCultivator

Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 13 years, 6 months
Re: Almost a month, nothing yet... [Re: drink_me]
    #2478525 - 03/27/04 04:47 PM (19 years, 10 months ago)

If you followed the pftek as close as you could, then you shouldn't have a problem with the moisture contant, shaking the syringe before is not too important in my opinion, I dont think thats the problem here.

Just give it another shot and incubate at at least 80F.

Erik006


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At last you know what ineffable is, and what ecstacy means


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InvisibleWormsy
Stranger
Registered: 03/24/04
Posts: 92
Re: Almost a month, nothing yet... [Re: drink_me]
    #2478576 - 03/27/04 05:11 PM (19 years, 10 months ago)

randomly you can get bunk spores, depends on how the spores were stored, etc.


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Offlinedrink_me
One swell chap

Registered: 02/23/04
Posts: 8
Loc: UK
Last seen: 17 years, 5 months
Re: Almost a month, nothing yet... [Re: Wormsy]
    #2479536 - 03/29/04 10:52 AM (19 years, 9 months ago)


Cheers guys,

I'll keep these ones going until my new spores turn up then give the new ones a go at a higher temp


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Invisibleoneducktwoducks
Registered: 12/13/02
Posts: 2,321
Re: Almost a month, nothing yet... [Re: drink_me]
    #2479616 - 03/29/04 11:32 AM (19 years, 9 months ago)

You sterilized in a pan?? Or do you mean a pot with a lid?


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Offlinedrink_me
One swell chap

Registered: 02/23/04
Posts: 8
Loc: UK
Last seen: 17 years, 5 months
Re: Almost a month, nothing yet... [Re: oneducktwoducks]
    #2480665 - 03/29/04 04:23 PM (19 years, 9 months ago)

Yeah I meant a pot with a lid


--------------------
"'You're like that Mynah' said Dr Robert at last, 'Trained to repeat words you don't understand or know the reason for...You've been told that we're just a set of self-indulgent dope-takers, wallowing in illusions and false samadhis. Listen, Murugan - forget all the bad language that's been pumped into you."
- Aldous Huxley, Island


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