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Offlineahkminlessr
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Panaeolus Spawn Contam In Bulk Substrate Help
    #23021549 - 03/18/16 08:26 PM (7 years, 10 months ago)

I am sure that my rye grain spawn of this Pan Camodgiensis appeared to be without any contamination when I shook and added to the pasteurized straw/poo substrate. However, the spawn jar was maybe a week or two past due, it had been incubating, after full colonization, for perhaps two weeks at about 70 deg F. I used the oven pasteurization method, 175 degrees F for two and a half hours. Substrate is 50 % chopped straw (rinsed and soaked for 1.5 hrs from a pre packaged Standlee chopped straw), 40% verm and 10% dehydrated horse manure from mycosupply, achieved to field capacity, covered in a turkey pan and into the oven for 2.5 hrs at 175 F. If you look, none of the bulk sub got contaminated, however...the completely (believed to be) clean inoculated grain spawn contaminated. This was about 4 days after the grain was added to the bulk, which was covered in aluminum foil and incubated in the dark for the spawn run. I can't tell what happened? The contamination is an olive/green colored mold ONLY on the grain. Is pH an issue? Very little grain actually ran onto the straw. Standlee says they don't use fungicides.None of the shakes to make the grain spawn had appeared to show contamination? Any ideas?


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