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OfflineMyko Fanatikos
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Pf to wbs slurry questions
    #23012031 - 03/16/16 08:44 AM (7 years, 10 months ago)



This is a pic of my wbs jars after 72 hours. There is no sign of contam and definite myc growing. I was in a hurry because my cakes started to pin invitro so I forgot to drill holes in the lids since i was pouring, and my slurry was like spooning mud into the jars. Can my slow colonization be from lack of Fae or not enough moisture? Could i just take a few cc of streile water and shoot it into my jars with a shake? Any help would be appreciated.


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OfflineMyko Fanatikos
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Re: Pf to wbs slurry questions [Re: Myko Fanatikos]
    #23012099 - 03/16/16 09:06 AM (7 years, 10 months ago)

Also I used foomans wbs tek for 10 jars and I used a whole of cake to 200ml of water so I could use more in each jar. I don't have 20 jars at the moment so I couldn't spread it out much.


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Invisibleeatyualive
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Re: Pf to wbs slurry questions [Re: Myko Fanatikos]
    #23016968 - 03/17/16 03:48 PM (7 years, 10 months ago)

if your using unmodified lids with no holes. make sure you leave them loose wile colonizing so that you can get GE. If they are tight, you won't get any GE at all and that will lead to contams.

pasty has a thread here.

https://www.shroomery.org/forums/showflat.php/Number/22767847

the mud is good. slurry is like mud. it also sticks to the grain that way allowing the entire jar to colonize in a few days.

don't inoculate anything else into the jars, don't open them, don't inject anything. it could be lack of GE. ideally you want to use a synthetic filter disk for your lids like so.

im not entirely sure how the slurry will react with unmodified lids. never tried it. it should work if you do it right in theory. i prefer these lids. don't go and drill holes now. you will have to setup everything prior to doing any transfers or pressure cooking your jars. be patient. don't rush these things.



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OfflineMyko Fanatikos
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Re: Pf to wbs slurry questions [Re: eatyualive]
    #23017623 - 03/17/16 06:46 PM (7 years, 10 months ago)

I posted this in eatyualive's thread.

https://www.shroomery.org/forums/postlist.php/Board/myposts

I got the same answer. I decided to just recap with sterilized lids with 1/8in holes and micropore tape. I explained it more in detail on the other thread. It worked well and took about 1second for each lid.

There is a definite change within 24 hours which may be attribute to GE or because it was pf slurry.

I didn't test it since it's my first attempt.


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OfflineMyko Fanatikos
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Registered: 02/18/16
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Re: Pf to wbs slurry questions [Re: Myko Fanatikos]
    #23019718 - 03/18/16 08:33 AM (7 years, 10 months ago)

Well I feel like an idiot, I gave you the link to your own thread :wink:

Is there any tek for making the lids your using?

Also great job on your recreation of tvcasualty's slurry tek. It took me a week to decide to do it and your thread was extremely helpful. I read it about 100 times over, Kudos to you sir.

I am literally just beginning but I have a good understanding of the sterile process and I've read enough in the past month to fill 3 people's minds. I am just getting pins from my very first run from brf cakes.

I think as long as someone is as sterile as possible and has a thorough understanding of the process, newbie or not; your tek is enough to make magic happen!


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Invisibleeatyualive
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Re: Pf to wbs slurry questions [Re: Myko Fanatikos]
    #23021486 - 03/18/16 07:57 PM (7 years, 10 months ago)

1) buy a synthetic filter disc.
2) use a nickel and a pen to race pieces as a template.
3) cut out pieces with scissors.
4) drill 1/4" hole in the center of your lid.
5) use high temp silicone or rtv silicone to glue the filters on. make sure that you glue around the edge so that the filter material covering the hole is clean
6) let it dry 24 hours. done.


there may be a thread out there. but i don't know.

also try loosening your lids. don't open them, but unscrew them a little. just make sure to remember to tighten them if you decide to pick them up. but loosen again once you put them down to incubate. see if it makes a difference.

i had some whatman ports die on me after about 10 years of pressure cooking. i then loosened the lids by unscrewing them a little. and the jars colonized fine. they were stalling out prior to that because they were not getting enough GE.


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