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Offlineshroomerian
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Green death after 3 tries...getting desperate...HELP!
    #18076033 - 04/08/13 10:12 AM (10 years, 9 months ago)

the story goes..

Had great success inoculating jars of rye with cakes in front of a flowhood with very careful sterile procedures..

last 3 times trich in every jar of rye...

been doing some agar too with great success after the infestation began and no mold!!

just in frecking jars...

last time i steilised for 150 min and again green death..

What the hell do i do?

Thought about leaving one jar uninoculated to see if the rye is in fact origin of spores or my procedures....

can mold spores survive that kind of sterilization? 

kernels turned dark brown....

fuck!! and i was doing so well...

did 5 grows without a single problem

need an advice!!


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Offlinekinkaku
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Re: Green death after 3 tries...getting desperate...HELP! [Re: shroomerian]
    #18076056 - 04/08/13 10:20 AM (10 years, 9 months ago)

well I don't really use rye but if its like wbs then you haave to soak it for a long period of time for the bacteria or whatever to get a foothold and to be more vulnerable when in the pc or you could just get different rye.


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OfflineKizzle
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Re: Green death after 3 tries...getting desperate...HELP! [Re: kinkaku]
    #18077005 - 04/08/13 01:56 PM (10 years, 9 months ago)

It's very unlikely for a sterilized substrate to be the source of the mold (bacteria would be another story), in fact at 150 mins it's virtually impossible. If you're getting it in every jar I'd say most likely your syringe is contaminated. Other possibilities would be a flaw in your sterile technique or a tiny chance it could be filter related.


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Offlinetokeweed420
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Re: Green death after 3 tries...getting desperate...HELP! [Re: Kizzle]
    #18078381 - 04/08/13 06:16 PM (10 years, 9 months ago)

Sorry bout your bad luck ......how long have you been doing this hobby ?imo its  either your rye was too wet are youve got a syringe that is  contaminated


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Offlineshroomerian
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Re: Green death after 3 tries...getting desperate...HELP! [Re: tokeweed420]
    #18080110 - 04/09/13 12:44 AM (10 years, 9 months ago)

i crumble cakes and throw in the jars..
cakes are colonised like a charm, strain is cambodian (fast)

been doing this few years now...
got my filter few months back..

filter is h13

agar with spores from syringe started to develop without contaminants..

will post pic


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OfflineJimmyhunter1000
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Re: Green death after 3 tries...getting desperate...HELP! [Re: shroomerian]
    #18081310 - 04/09/13 11:01 AM (10 years, 9 months ago)

When was the last time you gave your growing space a good wash down?


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Offlineshroomerian
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Re: Green death after 3 tries...getting desperate...HELP! [Re: Jimmyhunter1000]
    #18081373 - 04/09/13 11:17 AM (10 years, 9 months ago)

never?


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OfflineKizzle
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Re: Green death after 3 tries...getting desperate...HELP! [Re: Jimmyhunter1000]
    #18081580 - 04/09/13 12:12 PM (10 years, 9 months ago)

NM, I thought you were inoculating the rye directly with spores.

Make sure you're not touching the substrate at all during the transfer. Like if you're crumbing the cakes in your hands outside the bag (I assuming you're transferring from a bag) that would be an easy way to contaminate them. And also the outside the bag and the jar/lid needs to be thoroughly disinfected before you open either.


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Offlineshroomerian
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Re: Green death after 3 tries...getting desperate...HELP! [Re: Kizzle]
    #18081628 - 04/09/13 12:23 PM (10 years, 9 months ago)

yes..i clean thoroughly the glass (whitch the cake is in) with alcohol

i then put it in a ziplock, crumble and so on...

but i did it 5 times before with no problems....now suddenly green shit arises constantly

i've soaked the grains a bit longer now so that might help..

thanx everyone....maybe ill switch to wbs for the time being...never tried it....anyone has a decent tek?


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