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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
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developing a super strain: the worst part to deal with.
#13497602 - 11/17/10 10:56 AM (13 years, 2 months ago) |
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hi! i have the enough amount of time and space for develop a long-time project. my question is if someone working at tome, starting from a spore print of any mushroom (name it shiitake, reishi or another) could isolate, after thousands of attempts, a hidden treasure, name it a super strain.
which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
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Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
#13497663 - 11/17/10 11:08 AM (13 years, 2 months ago) |
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The worst part is that you never manage to find that super-strain, you overlook it or the features don't show up on agar.
Don't let that deture you though, it just means you'll have to fruit a lot of your isolates rather than just study them on agar?
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
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Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x] 1
#13500408 - 11/17/10 08:12 PM (13 years, 2 months ago) |
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You can isolate, test and save hundreds of strains per month easily. It doesn't take years. The problem is once you fruit your super strain and take a sporeprint, your super strain is gone. You have to keep it on master culture slants. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 11 hours, 30 minutes
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Re: developing a super strain: the worst part to deal with. [Re: RogerRabbit]
#13500923 - 11/17/10 10:02 PM (13 years, 2 months ago) |
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Hey RR, does a popsicle stick work for any mycelium?
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 2 hours, 54 minutes
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Re: developing a super strain: the worst part to deal with. [Re: Maverick]
#13502299 - 11/18/10 06:09 AM (13 years, 2 months ago) |
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thanks RR!
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
#13502326 - 11/18/10 06:23 AM (13 years, 2 months ago) |
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Quote:
x7x_x7x said:
which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?
No, weakening the gene pool.
--------------------
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 2 hours, 54 minutes
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Re: developing a super strain: the worst part to deal with. [Re: Mr. Mushrooms]
#14294644 - 04/15/11 09:05 AM (12 years, 9 months ago) |
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is rizomorphic micelium better than cloudy?
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
#14295020 - 04/15/11 11:04 AM (12 years, 9 months ago) |
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the work finding the super strain is in fruiting hundreds of thousands of isolates to find the one. Develop a small spawn run method (like PF TEC) and start running mini trial until you get your hit. Keep serious records and notes on each trial, quantify yield, observe diligently other behavior, like giant fruits or spore-less fruits, in general I think the stumbling upon the super strain is a matter of luck or providence in your efforts. It is like finding the needle in the haystack one straw at a time
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Microppose
Things Maker



Registered: 11/30/10
Posts: 849
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Re: developing a super strain: the worst part to deal with. [Re: mycoelf]
#14296024 - 04/15/11 02:45 PM (12 years, 9 months ago) |
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Had you discovered it, what would you consider a 'super strain'? 400% yield to substrate weight?
Edited by Microppose (04/15/11 02:45 PM)
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 2 hours, 54 minutes
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Re: developing a super strain: the worst part to deal with. [Re: Microppose]
#14296133 - 04/15/11 03:08 PM (12 years, 9 months ago) |
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i read several times in stamet's that the "better myceliums" are the rizomorphic ones, but i dont know if this concept mantains his validity nowadays.
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 2 hours, 54 minutes
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Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
#14296155 - 04/15/11 03:12 PM (12 years, 9 months ago) |
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i been shooting in the dark for years and think is time to become more precise.
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Medici_head
Void Explorer



Registered: 01/15/11
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Re: developing a super strain: the worst part to deal with. [Re: Mr. Mushrooms]
#14296419 - 04/15/11 04:13 PM (12 years, 9 months ago) |
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Quote:
Mr. Mushrooms said:
Quote:
x7x_x7x said:
which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?
No, weakening the gene pool.
He's right. There are only a finite number of theoretical passages an isolate can withstand before it dies out... But after talking with my advisor on this matter, cycling different nutrients in your agar will perpetuate the isolate longer allowing for extra passages... Now, I've never actually cycled my agar nutes before but I don't doubt his knowledge on the topic. medici
-------------------- Y o u ' r e a l l G o d s ; Y o u ' r e a l l G o d s ; Y o u ' r e a l l 2 m i l l i o n y e a r s o l d . ~T. Leary
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: developing a super strain: the worst part to deal with. [Re: Medici_head]
#14296581 - 04/15/11 04:48 PM (12 years, 9 months ago) |
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hats off for the great man who participated in this thread. I didn't notice it at first.
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sickofit
*statement*


Registered: 12/01/10
Posts: 348
Loc: Local Park
Last seen: 1 year, 4 months
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Re: developing a super strain: the worst part to deal with. [Re: cc2]
#14299107 - 04/16/11 12:51 AM (12 years, 9 months ago) |
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I wonder whats up with the guy who had a cube the size of his forearm. shit.
-------------------- A Resource on Psi, Science, and Philosophy The Taboo of Psi Trade/Want List Mechanisms and reactions Is ____ down, or is it just me? You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest! Done(In order): Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT
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Lord_McLovin
mad scientist on shrooms



Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
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Re: developing a super strain: the worst part to deal with. [Re: sickofit]
#14300234 - 04/16/11 10:01 AM (12 years, 9 months ago) |
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Quote:
sickofit said: I wonder whats up with the guy who had a cube the size of his forearm. shit.
Larger fruitbodies do in general not imply an increase in overall yield (as this depends more on the amount of pins formed before the first flush).
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sickofit
*statement*


Registered: 12/01/10
Posts: 348
Loc: Local Park
Last seen: 1 year, 4 months
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Re: developing a super strain: the worst part to deal with. [Re: Lord_McLovin]
#14302493 - 04/16/11 06:16 PM (12 years, 9 months ago) |
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Its still a trophy lol
-------------------- A Resource on Psi, Science, and Philosophy The Taboo of Psi Trade/Want List Mechanisms and reactions Is ____ down, or is it just me? You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest! Done(In order): Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT
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