Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinex7x_x7x
x7x, my problem child.
Male User Gallery


Folding@home Statistics
Registered: 05/05/07
Posts: 3,816
Loc: buenos aires Flag
Last seen: 2 hours, 54 minutes
developing a super strain: the worst part to deal with.
    #13497602 - 11/17/10 10:56 AM (13 years, 2 months ago)

hi! i have the enough amount of time and space for develop a long-time project. my question is if someone working at tome, starting from a spore print of any mushroom (name it shiitake, reishi or another) could isolate, after thousands of attempts, a hidden treasure, name it a super strain.

which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?


--------------------
cultivando en la miseria

SuctoSpore® Pictorial Tek


x7x_x7x@shroomery.org

carl_jung_in_lsd@yahoo.com

koh samui and oak ridge are my favourite strains


Extras: Filter Print Post Top
OfflineMaverick
Lover of Earwigs!
Male User Gallery


Folding@home Statistics
Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette Flag
Last seen: 11 hours, 30 minutes
Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
    #13497663 - 11/17/10 11:08 AM (13 years, 2 months ago)

The worst part is that you never manage to find that super-strain, you overlook it or the features don't show up on agar.

Don't let that deture you though, it just means you'll have to fruit a lot of your isolates rather than just study them on agar?


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 2 days
Trusted Cultivator
OG Cultivator
Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x] * 1
    #13500408 - 11/17/10 08:12 PM (13 years, 2 months ago)

You can isolate, test and save hundreds of strains per month easily.  It doesn't take years.  The problem is once you fruit your super strain and take a sporeprint, your super strain is gone.  You have to keep it on master culture slants.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineMaverick
Lover of Earwigs!
Male User Gallery


Folding@home Statistics
Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette Flag
Last seen: 11 hours, 30 minutes
Re: developing a super strain: the worst part to deal with. [Re: RogerRabbit]
    #13500923 - 11/17/10 10:02 PM (13 years, 2 months ago)

Hey RR, does a popsicle stick work for any mycelium?


Extras: Filter Print Post Top
Offlinex7x_x7x
x7x, my problem child.
Male User Gallery


Folding@home Statistics
Registered: 05/05/07
Posts: 3,816
Loc: buenos aires Flag
Last seen: 2 hours, 54 minutes
Re: developing a super strain: the worst part to deal with. [Re: Maverick]
    #13502299 - 11/18/10 06:09 AM (13 years, 2 months ago)

thanks RR!


Extras: Filter Print Post Top
InvisibleMr. Mushrooms
Spore Print Collector
 User Gallery

Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
    #13502326 - 11/18/10 06:23 AM (13 years, 2 months ago)

Quote:

x7x_x7x said:


which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?




No, weakening the gene pool.


--------------------


Extras: Filter Print Post Top
Offlinex7x_x7x
x7x, my problem child.
Male User Gallery


Folding@home Statistics
Registered: 05/05/07
Posts: 3,816
Loc: buenos aires Flag
Last seen: 2 hours, 54 minutes
Re: developing a super strain: the worst part to deal with. [Re: Mr. Mushrooms]
    #14294644 - 04/15/11 09:05 AM (12 years, 9 months ago)

is rizomorphic micelium better than cloudy?


--------------------
cultivando en la miseria

SuctoSpore® Pictorial Tek


x7x_x7x@shroomery.org

carl_jung_in_lsd@yahoo.com

koh samui and oak ridge are my favourite strains


Extras: Filter Print Post Top
Invisiblemycoelf
Agent Of Chaos
Male User Gallery


Registered: 06/26/09
Posts: 555
Loc: hyperspace Flag
Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
    #14295020 - 04/15/11 11:04 AM (12 years, 9 months ago)

the work finding the super strain is in fruiting hundreds of thousands of isolates to find the one. Develop a small spawn run method (like PF TEC) and start running mini trial until you get your hit. Keep serious records and notes on each trial, quantify yield, observe diligently other behavior, like giant fruits or spore-less fruits, in general I think the stumbling upon the super strain is a matter of luck or providence  :goodluck: in your efforts. It is like finding the needle in the haystack one straw at a time:headbang:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:


Extras: Filter Print Post Top
OfflineMicroppose
Things Maker
 User Gallery


Registered: 11/30/10
Posts: 849
Loc: Amongst you...
Last seen: 6 days, 13 hours
Re: developing a super strain: the worst part to deal with. [Re: mycoelf]
    #14296024 - 04/15/11 02:45 PM (12 years, 9 months ago)

Had you discovered it, what would you consider a 'super strain'?
400% yield to substrate weight? :lol:


Edited by Microppose (04/15/11 02:45 PM)


Extras: Filter Print Post Top
Offlinex7x_x7x
x7x, my problem child.
Male User Gallery


Folding@home Statistics
Registered: 05/05/07
Posts: 3,816
Loc: buenos aires Flag
Last seen: 2 hours, 54 minutes
Re: developing a super strain: the worst part to deal with. [Re: Microppose]
    #14296133 - 04/15/11 03:08 PM (12 years, 9 months ago)

i read several times in stamet's that the "better myceliums" are the rizomorphic ones, but i dont know if this concept mantains his validity nowadays.


Extras: Filter Print Post Top
Offlinex7x_x7x
x7x, my problem child.
Male User Gallery


Folding@home Statistics
Registered: 05/05/07
Posts: 3,816
Loc: buenos aires Flag
Last seen: 2 hours, 54 minutes
Re: developing a super strain: the worst part to deal with. [Re: x7x_x7x]
    #14296155 - 04/15/11 03:12 PM (12 years, 9 months ago)

i been shooting in the dark for years and think is time to become more precise.


Extras: Filter Print Post Top
OfflineMedici_head
Void Explorer
Male User Gallery


Registered: 01/15/11
Posts: 52
Loc: Flag
Last seen: 8 years, 11 months
Re: developing a super strain: the worst part to deal with. [Re: Mr. Mushrooms]
    #14296419 - 04/15/11 04:13 PM (12 years, 9 months ago)

Quote:

Mr. Mushrooms said:
Quote:

x7x_x7x said:


which would be the worst part of the project, other than years and tons of agar? the in vitro appearance of the tissue could predict the in vivo performance?




No, weakening the gene pool.




He's right.  There are only a finite number of theoretical passages an isolate can withstand before it dies out... But after talking with my advisor on this matter, cycling different nutrients in your agar will perpetuate the isolate longer allowing for extra passages... Now, I've never actually cycled my agar nutes before but I don't doubt his knowledge on the topic. medici


--------------------
Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  2  m i l l i o n  y e a r s  o l d . ~T. Leary


Extras: Filter Print Post Top
Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Re: developing a super strain: the worst part to deal with. [Re: Medici_head]
    #14296581 - 04/15/11 04:48 PM (12 years, 9 months ago)

:hatsoff: hats off for the great man who participated in this thread. I didn't notice it at first. :bow2:


Extras: Filter Print Post Top
Offlinesickofit
*statement*
Male User Gallery

Registered: 12/01/10
Posts: 348
Loc: Local Park Flag
Last seen: 1 year, 4 months
Re: developing a super strain: the worst part to deal with. [Re: cc2]
    #14299107 - 04/16/11 12:51 AM (12 years, 9 months ago)

I wonder whats up with the guy who had a cube the size of his forearm. shit.


--------------------
A Resource on Psi, Science, and Philosophy
The Taboo of Psi
Trade/Want List
Mechanisms and reactions
Is ____ down, or is it just me?
You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest!
Done(In order):
Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT


Extras: Filter Print Post Top
OfflineLord_McLovin
mad scientist on shrooms
Male User Gallery


Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 1 month
Re: developing a super strain: the worst part to deal with. [Re: sickofit]
    #14300234 - 04/16/11 10:01 AM (12 years, 9 months ago)

Quote:

sickofit said:
I wonder whats up with the guy who had a cube the size of his forearm. shit.




Larger fruitbodies do in general not imply an increase in overall yield (as this depends more on the amount of pins formed before the first flush).


--------------------
Stand up. You're not alone.


Extras: Filter Print Post Top
Offlinesickofit
*statement*
Male User Gallery

Registered: 12/01/10
Posts: 348
Loc: Local Park Flag
Last seen: 1 year, 4 months
Re: developing a super strain: the worst part to deal with. [Re: Lord_McLovin]
    #14302493 - 04/16/11 06:16 PM (12 years, 9 months ago)

Its still a trophy :mushroom2: lol


--------------------
A Resource on Psi, Science, and Philosophy
The Taboo of Psi
Trade/Want List
Mechanisms and reactions
Is ____ down, or is it just me?
You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest!
Done(In order):
Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 9,131 18 07/24/01 12:02 AM
by Anno
* Super Spawn? Maybe. **UPDATED 1/4**
( 1 2 3 all )
altarego 14,078 44 05/13/12 12:52 AM
by CheeWiz
* Re: Developing a fruiting strain of Psilocybin Mexicana! AnubisRonin 2,044 10 02/06/00 11:55 AM
by Ryche Hawk
* super potent shrooms audiophile 3,225 16 09/13/01 01:17 PM
by Anonymous
* Crossing two strains IS possible..Heres how...
( 1 2 3 4 5 all )
BlueJay 34,962 96 06/01/15 10:34 PM
by God25
* Re: Mushroom Strain Improvement and Breeding? Ripper 4,878 10 02/10/00 07:01 AM
by Anonymous
* Statistical spread of potency*yield/time of isolated strains of Cubensis. Baby_Hitler 334 3 03/02/18 03:34 PM
by bodhisatta
* new strain developed at theforestfloor shannonxmas 1,273 9 01/30/02 11:00 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
2,125 topic views. 2 members, 11 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 15 queries.