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OfflineFyasko
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This Can't Be Normal...[Healthy Mycelium?]
    #11936823 - 01/31/10 04:47 PM (13 years, 11 months ago)

Hai guyz -

I need some help identifying this as a contam or healthy mycelium. Pretty sure it isn't normal, but I haven't seen a contam like this described anywhere. Here are a couple pictures of two jars both knocked up with Ecuadorians. Obviously the trouble is with the jar on the left...



The spot where the contam is looks as if it has dented or sunken the myc, which is what leads me to believe it is a contam and not just myc piss.

As an added note: I know that my jars are FAR too wet, like a dumbass I forgot to foil some of the lids during sterilization. Wasn't counting on them being perfect, but hoping they would tough it out.


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Offlinemushking
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11936865 - 01/31/10 04:55 PM (13 years, 11 months ago)

personally i think that whole jar looks fucked all to shit.


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OfflinePureGrit
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11936866 - 01/31/10 04:56 PM (13 years, 11 months ago)

looks like the mud contam.


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Invisibleanonjon
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: PureGrit]
    #11936917 - 01/31/10 05:05 PM (13 years, 11 months ago)

prolly bacterial if was overly wet.

toss it.


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: anonjon]
    #11937029 - 01/31/10 05:31 PM (13 years, 11 months ago)

Alright alright alright, just hearing what I didn't want to hear. It's garbage, thanks guys.


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OfflineLogicaL ChaosM
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11937056 - 01/31/10 05:36 PM (13 years, 11 months ago)

Quote:

Fyasko said:
I need some help identifying this as a contam or healthy mycelium. Pretty sure it isn't normal, but I haven't seen a contam like this described anywhere. Here are a couple pictures of two jars both knocked up with Ecuadorians. Obviously the trouble is with the jar on the left...

As an added note: I know that my jars are FAR too wet, like a dumbass I forgot to foil some of the lids during sterilization. Wasn't counting on them being perfect, but hoping they would tough it out.




Yeah, I can see in this photo its WAY too wet! (that's what he said?)

I can see the water line about 1/4 up the jar, so that jar is drowning right now. There's your cause. Next time you sterilize your jars, make sure they can't fall down in the boiling water. And of course, make sure the foil covers the jar top *completely*. Thats important.

Time to Toss It,
~ LogicaL Chaos ~


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OfflineOMJebus
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: LogicaL Chaos]
    #11937075 - 01/31/10 05:40 PM (13 years, 11 months ago)

how long ago did you noc those jars up? Just curious


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11937118 - 01/31/10 05:49 PM (13 years, 11 months ago)

Knocked up 20 jars on the 4th. 4 strains in 5 jars/ea. B+, GT, Ecuadorian, Treasure Coast. Had a total of 4 jars that didn't even BEGIN to grow myc, probably due to many problems during innoc. The main problem I had was my verm clogging the syringe and I'm guessing that I didn't manage to get spores in those jars. Despite most of the jars being too wet the B+ and GT's seems to have very few problems. The TC's are inching along, even in my TiT [temp stays between 75-80]. Any suggestions?


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OfflineLogicaL ChaosM
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11937155 - 01/31/10 05:57 PM (13 years, 11 months ago)

Quote:

Fyasko said:
Knocked up 20 jars on the 4th. 4 strains in 5 jars/ea. B+, GT, Ecuadorian, Treasure Coast. Had a total of 4 jars that didn't even BEGIN to grow myc, probably due to many problems during innoc.

The main problem I had was my verm clogging the syringe and I'm guessing that I didn't manage to get spores in those jars.

Despite most of the jars being too wet the B+ and GT's seems to have very few problems. The TC's are inching along, even in my TiT [temp stays between 75-80]. Any suggestions?




I kinda confused by this statement: How can you not tell if you injected spores passed the vermiculite? If the vermiculite is clogging it, just back up the syringe a bit and you should be fine. Also, did you inject the spores at the edge of the jars? Maybe thats why you can't tell if spore solution came out.

Also, the incubation temp is a little high at 80 degrees F. The inside of the jar will actually be hotter because of the mycelium creates heat as it grows. 75F to 78F is ideal range.

So, out of the 20 jars, how many jars are actually growing white mycliem?

Let us know,
~ LogicaL Chaos ~


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OfflineSpongiform
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: LogicaL Chaos]
    #11937191 - 01/31/10 06:06 PM (13 years, 11 months ago)

The left jar looks rotten, right jar looks fine to me.


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Offlinepunkrocker292004
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Spongiform]
    #11937242 - 01/31/10 06:17 PM (13 years, 11 months ago)

Quote:

Spongiform said:
The left jar looks rotten, right jar looks fine to me.




nvm i always mix up right and left:facepalm:


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Edited by punkrocker292004 (01/31/10 06:20 PM)


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: LogicaL Chaos]
    #11937427 - 01/31/10 06:54 PM (13 years, 11 months ago)

Quote:


I kinda confused by this statement: How can you not tell if you injected spores passed the vermiculite? If the vermiculite is clogging it, just back up the syringe a bit and you should be fine. Also, did you inject the spores at the edge of the jars? Maybe thats why you can't tell if spore solution came out.

Also, the incubation temp is a little high at 80 degrees F. The inside of the jar will actually be hotter because of the mycelium creates heat as it grows. 75F to 78F is ideal range.

So, out of the 20 jars, how many jars are actually growing white mycliem?

Let us know,
~ LogicaL Chaos ~





After I inserted the syringe into the jars for innoc [outwards, toward the sides, for a clear view of the innoc point] I obviously tried to push the plunger on the syringe, but it wasn't just "blocked" at the end. The syringe was literally filled with tiny pieces of verm, not allowing me to even push the syringe down. There were a couple jars that received more solution than intended because of this problem. However, I was not aware that 4 jars received ZERO spores. If I ran into this problem, I didn't just skip the jars, I attempted to unclog and re-inject the jars. Overall, the innoc was rushed and I had the aid of the ignorant, so I'm not surprised about some of my contams. I am surprised at the lack of four jars to produce ANY mycelium.

Here's a breakdown [of 20 overall jars]:

B+:
--  5/5 with healthy, almost fully colonized myc.

Treasure Coast:
--  4/5 with healthy, but slow growing myc.
--  1/5 with contaminated [forest green mold], but growing myc.

Golden Teacher:
--  3/5 with healthy, almost fully colonized myc.
--  1/5 with questionable, almost fully colonized myc.
--  1/5 with absolutely zero mycelium growth, what-so-ever.

Ecuadorian:
--  3/5 with absolutely zero mycelium grow, what-so-ever.
--  1/5 with contaminated, but fully colonized myc.
--  1/5 with healthy, fully colonized myc.


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Invisibleanonjon
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11937637 - 01/31/10 07:35 PM (13 years, 11 months ago)

You know to unclog a syringe u pull up on the plunger, right?


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: anonjon]
    #11937710 - 01/31/10 07:51 PM (13 years, 11 months ago)

Quote:

anonjon said:
You know to unclog a syringe u pull up on the plunger, right?




Yeah uh easier said than done.


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Invisiblefeelfunny
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: anonjon]
    #11937716 - 01/31/10 07:52 PM (13 years, 11 months ago)

Quote:

anonjon said:
You know to unclog a syringe u pull up on the plunger, right?




im sure pushing out would work to


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OfflineLogicaL ChaosM
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: feelfunny]
    #11937925 - 01/31/10 08:25 PM (13 years, 11 months ago)

Quote:

feelfunny said:
Quote:

anonjon said:
You know to unclog a syringe u pull up on the plunger, right?




im sure pushing out would work to




Don't you mean pulling-out?

My advice to avoid the needle tip from clogging is to drill the holes for the half-pint jars *right* next the edge of the lid. That way, you can slide the needle tip *right* on the glass wall fo the jar. I never have problems with clogging that way. Also, make sur the open side of the tip is facing toward you, so you can see if the solution is being injected out.

If you follow that, you shouldn't have any clog problems. I can contest that I haven't.

Good Luck Next Time,
~ LogicaL Chaos ~


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: LogicaL Chaos]
    #11937984 - 01/31/10 08:33 PM (13 years, 11 months ago)

Quote:

My advice to avoid the needle tip from clogging is to drill the holes for the half-pint jars *right* next the edge of the lid. That way, you can slide the needle tip *right* on the glass wall fo the jar. I never have problems with clogging that way. Also, make sur the open side of the tip is facing toward you, so you can see if the solution is being injected out.

If you follow that, you shouldn't have any clog problems. I can contest that I haven't.

Good Luck Next Time,
~ LogicaL Chaos ~




Thanks for the advice, going to try this next time. Definitely a problem as this happened at my last innoc. Didn't even really think about it like that, but do believe this to be the problem. Thanks for all the help, will post the results of the grow.


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OfflineLogicaL ChaosM
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Fyasko]
    #11938248 - 01/31/10 09:04 PM (13 years, 11 months ago)

No prob dude.

So that you have an idea of what it should look like, I took some pictures of my jar lids for ya....


^ Note that the jar lid is upside-down. I did this so its easier to see the holes I drilled. The other side is white.


^ Make sure the open part of the needle tip faces out toward the glass. That will prevent clogging and you can see where the spore solution actually goes.

If you got any questions, feel free to ask.

Hope that helps you out

~ LogicaL Chaos ~


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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: LogicaL Chaos]
    #11940198 - 02/01/10 03:31 AM (13 years, 11 months ago)

ok I use only fine grade verm because of availability so lemme tell ya a few things about clogged syringes. This is all IME

1. The spores pass thru fine. the clog won't filter them out they are tiny. I'v never had a dead spot from a clogged syringe. Ironically my dead spots always happen on my inoculations  where everything goes perfectly ><

2. THE PERFECT WAY TO CLEAR A NEEDLE: just pull the fucker out and heat up the bottom of the needle with a lighter. Whatever inside will get pushed out like a bullet. You will see it and hear it.  Every so often you'll bake a piece of BRF in yer needle :p it happens just push it out it dosen't fight back hard. This is IMHO

3. You can always just push real hard and accidentally shoot a CC and a half into your jar.  Don't worry about it its never casued me problems.

4. Have a bowl packed because I don't know about you but it PISSES ME OFF!!


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OfflineFyasko
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Re: This Can't Be Normal...[Healthy Mycelium?] [Re: Grumpyorc]
    #11944804 - 02/01/10 09:14 PM (13 years, 11 months ago)

Quote:

Grumpyorc said:
ok I use only fine grade verm because of availability so lemme tell ya a few things about clogged syringes. This is all IME

1. The spores pass thru fine. the clog won't filter them out they are tiny. I'v never had a dead spot from a clogged syringe. Ironically my dead spots always happen on my inoculations  where everything goes perfectly ><

2. THE PERFECT WAY TO CLEAR A NEEDLE: just pull the fucker out and heat up the bottom of the needle with a lighter. Whatever inside will get pushed out like a bullet. You will see it and hear it.  Every so often you'll bake a piece of BRF in yer needle :p it happens just push it out it dosen't fight back hard. This is IMHO

3. You can always just push real hard and accidentally shoot a CC and a half into your jar.  Don't worry about it its never casued me problems.

4. Have a bowl packed because I don't know about you but it PISSES ME OFF!!





QUOTED for fucking truth man. I found that this helped me, and you're right, I squirted like a CC and half into a couple holes [lol]. Glad to know that the problem isn't just mine. :laugh:


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