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Unknownfungi
Amateur mycologist



Registered: 09/28/21
Posts: 584
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Gymnopilus luteofolius potency test results 1
#27962575 - 09/22/22 02:02 PM (1 year, 4 months ago) |
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We're back again with a special episode of "HOW STRONG IS THAT?"
Today's show is dedicated to Mentalpariah whom patiently awaits these results. What species could Unknown be testing you ask? Well wait no further, the star of the episode is a mushroom genus near and dear to many of us... GYMNOPILUS!! Which species? Why luteofolius of course. Now onto the good stuff.
Gymnopilus luteofolius was collected 9/16 and set in the dehydrator within an hour of picking. They dried for 10hrs at 68c. Once dried and labeled the samples were vacuum packed.
PREP
Sample preparation was conducted by first weighing out 2g of caps and stipes and both small and large fruits. The sample was then finely ground. This is done to ensure a more homogeneous result as potency levels vary between mushrooms. 150mg of fine powder was weighed out from the original 2g. This will be our testing material.

TESTING
The 150mg of testing material was added to the extraction solvent. This was shaken periodically over the period of ten minutes.

1.0ml of the extract was filtered using a 1.0ml syringe fitted with a Sterifit to remove any solids.

This was then added to the bottle of quantitative reagent. Reagent was placed in a hot water(90c) bath for fifteen minutes to react.

After fifteen minutes the bottle was removed from the bath. A color change was observed indicating psilocybin content. The color was then compared to the included chart.

RESULTS
To me the color seems to best match between 0.6%-0.9% psilocybin or 6mg-9mg psilocybin per gram dried. The color is a bit darker than 0.6% but not as dark as 0.9% which puts it very close to Gymnopilus luteus which tested roughly 0.6%. Much stronger than most think. I do believe the secondary chemicals play a role.
As always I'd love to hear others opinions/thoughts on what potency they think matches best. I hope you all enjoyed this episode of "HOW STRONG IS THAT?"
I don't know how long until the next episode as I'm out of test kits and stressed monetarily. I appreciate all the love surrounding my posts and I love doing it for the community. Until next time everyone
Edited by Unknownfungi (09/22/22 03:14 PM)
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Moria841



Registered: 07/02/18
Posts: 4,928
Loc: NJ
Last seen: 6 hours, 9 minutes
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Re: Gymnopilus luteofolius potency test results [Re: Unknownfungi]
#27962792 - 09/22/22 04:25 PM (1 year, 4 months ago) |
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Very well done, so about the strength of other active gymns! Definitely interesting
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Typerwritermonky
shboop a doop a doop


Registered: 01/19/12
Posts: 5,375
Loc: Mrs. Brown's Teahouse
Last seen: 1 day, 13 hours
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Re: Gymnopilus luteofolius potency test results [Re: Moria841]
#27962815 - 09/22/22 04:46 PM (1 year, 4 months ago) |
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To be honest my eyes see between .3-.6, I feel like it's a solid .5%. I've read about people doing a color match so you take the picture in a software like photoshop, and get an average mean of the color within the vial by sampling it's color code. You then compare that to the color code of the piece of paper and it's spectrum and get the most unbiased answer.
I love these posts by the way, so cool!
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Carnonos
The Horned Wanderer

Registered: 02/05/23
Posts: 55
Loc: Terra
Last seen: 17 days, 8 hours
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Re: Gymnopilus luteofolius potency test results [Re: Unknownfungi]
#28416001 - 08/01/23 10:01 AM (5 months, 24 days ago) |
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Just showed this test to a buddy of mine the other day who's going to be hunting with me this fall. The guy's a homebrewer and he had an interesting idea to help figure out what color this test is registering... Apparently for homebrew competitions, color of beer in a glass can be measured using a scale called SRM, and they even have judging apps for homebrewers that can roughly measure it using your phone's camera. He then whipped out his phone and pulled up his app and used it on a couple of your photos...
here's the scale he got for the test card:
o mg - 1 srm 3 mg - 6 srm 6 mg - 10 srm 9 mg - 13 srm 12 mg - 16 srm 15 mg - 20 srm
and the sample bottle: 10-11 srm
He used the 1st and 4th pic primarily, said they had less "visual distractions" like shadows and refraction being seen in the bottle and the color was more consistent. Also said that doing the test off a computer screen would make it "kinda wonky" to get accurate readings, but I thought it was interesting anyway to see.
Edited by Carnonos (08/01/23 10:04 AM)
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Pentacorn
Lover


Registered: 09/18/22
Posts: 174
Loc: Upper Midwest
Last seen: 1 day, 21 hours
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Re: Gymnopilus luteofolius potency test results [Re: Carnonos]
#28416457 - 08/01/23 04:19 PM (5 months, 24 days ago) |
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Thanks for this! I'm still hoping the luteofolius that is eating my porch will give me some fruits this summer but no luck so far.
-------------------- ----------------------------------------------------------------- WILD LAWN PANAEOLUS - ACTIVE OR NOT? See discussion here: Panaeolus Identification
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Moria841



Registered: 07/02/18
Posts: 4,928
Loc: NJ
Last seen: 6 hours, 9 minutes
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Re: Gymnopilus luteofolius potency test results [Re: Pentacorn]
#28416462 - 08/01/23 04:22 PM (5 months, 24 days ago) |
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Quote:
Pentacorn said: Thanks for this! I'm still hoping the luteofolius that is eating my porch will give me some fruits this summer but no luck so far.
Water your porch every morning and afternoon
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Longtimenosee


Registered: 12/05/22
Posts: 254
Last seen: 1 day, 9 hours
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Re: Gymnopilus luteofolius potency test results [Re: Moria841]
#28416754 - 08/01/23 08:48 PM (5 months, 24 days ago) |
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Your G. luteofolius may be a G. aeruginosa. It has blue green spots on the caps and a white cap and stem context which are the features used to distinguish G. aeruginosa from G. luteofolius according to the original description of the species.
Edited by Longtimenosee (08/01/23 08:48 PM)
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Icyurmt
Strange


Registered: 04/02/20
Posts: 1,625
Loc: 5a
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Re: Gymnopilus luteofolius potency test results [Re: Longtimenosee]
#28416879 - 08/01/23 10:47 PM (5 months, 24 days ago) |
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-------------------- 👁️ 🌊 why you are empty. Hunt for the habitat not the mushroom.
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Moria841



Registered: 07/02/18
Posts: 4,928
Loc: NJ
Last seen: 6 hours, 9 minutes
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Re: Gymnopilus luteofolius potency test results [Re: Icyurmt] 1
#28416893 - 08/01/23 11:10 PM (5 months, 24 days ago) |
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I highly doubt G. aeruginosa is an actual species tbh
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Longtimenosee


Registered: 12/05/22
Posts: 254
Last seen: 1 day, 9 hours
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Re: Gymnopilus luteofolius potency test results [Re: Moria841]
#28417203 - 08/02/23 08:41 AM (5 months, 23 days ago) |
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It maybe the same, but the features here are inline with G. aeruinosus. It seams like using the name G. aeruginosus for specimens with white context and blue-green dots on the caps would be appropriate until they can be proven synonymous. Just my opinion. I do not like to assume certain names are synonymous without proof. A ITS sequence comparison between those with colored context and those without would be helpful.
Regardless cool post thanks for sharing!
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