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Invisibleeatyualive
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Cornmeal Plate Liquid Inoculation * 4
    #22252599 - 09/17/15 06:31 PM (8 years, 7 months ago)

:chief:CORNMEAL PLATE AND
BROWN RICE FLOUR PLATE
LIQUID INOCULATION :chief:





Brought to you by THE TRIBE


Let me begin by saying that this incredible idea here has given me some inspiration to try something different. Thanks, Psilo!

Tortilla Tek

Liquid Inoculation( in case you didn't know)

Liquid Inoculation

Goals:

The overall goal is to have the fastest colonization speed for quarts available. This creates consistency, simplicity, speed and ease of use. Anything that helps you reduce work load is a benfit. Cornmeal Plate Liquid Inoculation accomplishes this. Cornmeal plate Liquid Inoculation method allows you to colonize your quart grain jars in 5 days without adding anything to your grain or without having to shake the grain jars. The method is 1-2  days slower than Slurry inoculation but you don't have to make any pf jars.

The examples below use Whole Race Horse Oats as the grain. Somehow the added nutrients from the cornmeal combined with the viscosity of the liquid inoculant allow leap off to show after 24 hours time. My theory is that the thicker viscosity of the slurry and cornmeal blends allow the inoculant to stick or cling to the grain better. Where you may have a regular agar plate blend slip down the side of the grain. This is by far the fastest leap off times I have seen with Liquid Inoculant by itself without any additives in the grain.

Psilopsychin, Grey and Insanemike came up with this cool idea to try LI using these cornmeal mush plates. This thread is dedicated specifically to the cornmeal plate Liquid Inoculation using cornmeal, malt o meal, or brown rice flour. Feel free to post anything related to preparing grain with these types of high nutrient liquid inoculants.


Summary of Procedure:

1. All cultures were started from spores on pasty's easy agar tek recipe with 2 transfers prior to making a 3rd transfer on a cornmeal plate. This was to visually show clean culture prior to making Liquid inoculation.
2. The 3rd transfer used the cornmeal recipe below with a higher nutrient source.
3. Colonize the clean culture on cornmeal plate to about 1/8" from the edge of the mini round.
4. Setup a SAB and Tiger Drop your cornmeal plate into a regular mouth 1/2 pint jar with oster blender lid attachment.
5. Blend the jar on your oster blender.
6. Liquid inoculate your grain jars.

RECIPE for 8 glad mini rounds:
  • 4 tablespoons cornmeal
  • 1 cup water



Items Needed:

  • Blue organic cornmeal(optional regular color cornmeal with food coloring)
  • 8 glad mini rounds(preferably lids cut like pasty plates)
  • 1 cup water
  • a small pot
  • 1 tablespoon and 1 cup measuring utensils
  • spoon


Procedure:
1. Measure 4 tablespoons blue organic cornmeal. (**You can optionally add food coloring like pasty's mix if you can only find regular colored cornmeal)


2. Measure 1 cup water. Dumped it in the pot.


3. Using a small pot, Put the heat on high for one minute.

4. Dump the cornmeal in and stir the cornmeal into the water.


5. As soon as the cornmeal started bubbling(maybe 2 minutes) heat was reduced to low. You can see a nice even thickness and blend to the cornmeal.


6. The cornmeal is pour able but thick. Almost the consistency of paper mache paste. Use a spoon to push and pour the cornmeal into 8 glad mini rounds.


7. Once settled for about 30 seconds. LIft each mini round and kind of hit the bottom of it against the surface of the cutting board in order to consolidate the mush a little more even in the plate. You can lightly repeat this a few times to settle the cornmeal in the bottom of the plate. It won't be perfectly even but as even as you can get it.



8. The rounds are put in the pc and pressure cooked for 45 mins at 15psi.

9. After transferring clean cultures on regular Pasty's easy agar tek recipe, 3rd transfers from agar were placed onto the cornmeal plates. The cultures were observed so that no visible contamination was present.



You can see the growth on the 3rd transfer cornmeal plates below. , AA+ cubensis,
  • AA+



10. A tribal container can be built like this.Tribal Container

Or you can use a simple 1/2 pint jar/blender attachment build below. In this example I used a simple build. I used an oster blender attachment that was rtved to a regular mouth band. The tribal container is easier to use.


Water to Plate Ratio for Liquid Inoculant:

  • 1/2 pint regular mouth jar used
  • 1/4 pint water or fill the half pint halfway
  • 1 colonized cornmeal agar plate


A regular mouth 1/2 pint was filled half full with water (1/4 pint water to 1 cornmeal agar plate). The blue pour lid shown below was wrapped in foil. Both items were pressure cooked for 20 minutes at 15psi. Once cool. Clean area(SAB) was setup. All transfers and tiger drops were taken in the SAB.

The lid to the receiving jar was lifted. The plate was Tiger Dropped into the jar with blender attachment. The jar was taken out of SAB then the jar was blended, then brought back into the SAB. The blender lid was swapped with a pour lid and transfers were done. If you had a tribal container, you could drop the plate right in, blend and pour. Easy as 123. Tribal container FTW!

Regular Mouth Blade attachment sealed with rtv silicone to a regular mouth band lid.





11. Blended cornmeal plate as a Liquid Inoculant Then poured a quick pour into each grain jar. Around 2-3ccs by eye. You can see a video on pouring the liquid inoculant in a SAB in the tribal container thread by Carcase-x. I posted it below for illustration.

t=220

The image below is an example of a 1/2 pint jar being blended on an oster blender. This is for reference to show you what it looks like.



The image below is a Cornmeal Plate after a blend, ready for Liquid Inoculation




CORNMEAL MUSH PLATE GROWTH IN GRAIN JARS


Below is Side by Side 5 Day Growth with 2 Cubensis cultures

  • SYZYGY CLONE 1:


  • AA+ :




Tested Bulk Recipe for 10 tubs:

  • 6 small bricks coir
  • 8 quarts verm,
  • 2 quarts gypsum,
  • 4 quarts dung
  • 50 quarts of water at 5 quarts per tub.


The substrate wasOven pasteurized

The fruiting method used is shown in this thread by Azur. Case at Spawning with polyfil

The grow environment is humid with 50% relative humidity in the grow room. Adapt as needed for your environment.

The tubs were colonized with medium polyfil in all holes that were put in during spawning. The tubs were left in a closet with no fan on.  This means you can colonize your bulk, pin, fruit and harvest without doing anything to the polyfil(old method ive used forever and it works perfectly in a humid environment)



Fruiting was induced at 30-50% casing colonization. The fan and light were turned on with a 12/12 light timer and 3 hours on and 30 minutes off for the fan.



AA+ Pinning and fruiting

Tub 1


Tub 2


Tub 3



2nd Flush:



TRIAL #2:



Water to Plate Ratio for Liquid Inoculant:

  • 1 pint regular mouth jar used
  • 1/2 pint water or fill the pint halfway
  • 1 colonized cornmeal agar plate


A regular mouth pint jar was used, filled half full with water. This is double the water volume from trial #1 and the jars took 4-5 days extra to colonize without a shake. This was not the ideal result. This method will inoculate more jars but will take longer to colonize.





Trial 2 success!









Brown Rice Flour Plate Liquid Inoculation:


RECIPE for 8 glad mini rounds:
  • 4 tablespoons brf
  • 1/2 cup water



Items Needed:

  • Brown Rice Flour

  • A small bowl
  • 8 glad mini rounds(Pasty Plates)
  • 1/2 cup water

  • a small pot

  • 1 tablespoon and 1 cup measuring utensils

  • spoon
  • Food Coloring


Prep Time:2 Minutes

Procedure:

1. Get a small bowl and measure 4 tablespoons of brown rice flour. place it in the bowl.


2 Measure out 1/2 cup of water. Pour the water into the small pot. Place the pot on the stove. Put the stove on medium heat.


3. Pour the brown rice flour into the pot with water.


4. Use a small spoon to mix the brown rice flour into the water until it becomes an even, smooth paste.


5. Add a few drops of food coloring of your choice. Stir the food coloring in until the color is even. As you are stirring your brown rice flour paste is heating up. (you are only about 1 minute into the cook)


6. You will notice that the mixture is solidifying and becoming thicker. This is great.


7. Take the paste off the heat. By now the paste is almost medium solid and the consistency of grits. Take a small spoon and evenly distribute s spoonful into each plate.



8. After you have placed a spoonful of paste into each plate. Lightly tap the plate against an even hard surface like a counter top. Continue doing this until the paste evens out. Now you are done. This took 2 minutes total.


9. Use a damp paper towel to wipe any paste that was spilled on the sides of the plates.

9. Pressure cook for 45 minutes at 15psi.

Edited by eatyualive (03/04/16 08:31 PM)

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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22254092 - 09/18/15 12:05 AM (8 years, 7 months ago)

Looking Good!
Maybe add a link in the OP up there to your tek for making that LI blender jar?


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Offlinepurplecap
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22255081 - 09/18/15 08:36 AM (8 years, 7 months ago)

That growth is sick fast in the first set of pics. You guys might have just found the fastest way to get spawn.

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Invisiblepaperbackwriter
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Registered: 03/31/14
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: purplecap]
    #22255725 - 09/18/15 11:01 AM (8 years, 7 months ago)

To contribute here's Mexicana on mush.  I followed eat's ratios, used plain Quaker Oats cornmeal, and added a drop or two of blue food coloring to get it green like that.

This particular wedge got stuck on the side and had to be banged around to get centered.  Hence the satellite growth.

The pic is from yesterday.  I'll take another before I drop it.



--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley

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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22255801 - 09/18/15 11:25 AM (8 years, 7 months ago)

Quote:

PsiloPsychIn said:
Looking Good!
Maybe add a link in the OP up there to your tek for making that LI blender jar?



Yeah man ill do that tonight. Its in the tribal blender thread. Thanks psilo.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22255823 - 09/18/15 11:32 AM (8 years, 7 months ago)

After a bunch of trials ill move this to mush cult. Ill post grows and everything.

Quote:

purplecap said:
That growth is sick fast in the first set of pics. You guys might have just found the fastest way to get spawn.





Its not aa fast as pf jar slurry but its close! Im hoping adding less water to the mix will change that


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Edited by eatyualive (09/18/15 11:37 AM)

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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22256022 - 09/18/15 12:35 PM (8 years, 7 months ago)

Ok updated with a link to carcs container build along with my blender setup.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22258008 - 09/18/15 08:34 PM (8 years, 7 months ago)

update

Below is a Side by Side with 4 different cultures. Syzygy Clone 1 Cubensis, AA+ cubensis, Panaeolus (Copelandia) cyanescens : Jamaican, Psilocybe tampanensis

  • SYZYGY CLONE 1:12hrs-48 hours


  • AA+ 12hrs-36 hours


  • PAN. JAMAICA 12hrs-36 hours


--------------------
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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22275670 - 09/22/15 06:46 PM (8 years, 7 months ago)

updated. leap off in 24 hours from the p. tamps!

Below is Side by Side Growth with 4 different cultures. Syzygy Clone 1 Cubensis, AA+ cubensis, Panaeolus (Copelandia) cyanescens : Jamaican, Psilocybe tampanensis


CORNMEAL MUSH PLATE LIQUID INOCULATION


  • SYZYGY CLONE 1: Day 1-5


    [LIST]
  • AA+ : Day 1-7


  • PAN. JAMAICA Day 1-8


  • P. TAMP. Day 1-2




--------------------
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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22275747 - 09/22/15 06:59 PM (8 years, 7 months ago)

Nice!
Impressive jump off in 24 hours on that Ps. Tamp.
It all looks great Eat!
:awedance:


--------------------
What are they saying? Listen carefully, it might be something you need to hear...




Edited by PsiloPsychIn (09/22/15 08:37 PM)

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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22275791 - 09/22/15 07:08 PM (8 years, 7 months ago)

its consistent on all 4! im loving these so much, i already made 8 more for when i get back from vacation! :thumbup:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22276351 - 09/22/15 09:01 PM (8 years, 7 months ago)

also i made 8 plates. with about 1/4 cup less water than the previous mix.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22281113 - 09/23/15 09:06 PM (8 years, 7 months ago)

updated tamps and pan jamaica


CORNMEAL MUSH PLATE LIQUID INOCULATION


Below is Side by Side Growth with 4 different cultures. Syzygy Clone 1 Cubensis, AA+ cubensis, Panaeolus (Copelandia) cyanescens : Jamaican, Psilocybe tampanensis

  • SYZYGY CLONE 1: Day 2-5


  • AA+ : Day 2-7


  • PAN. JAMAICA Day 2-8


  • P. TAMP. Day 2-4




--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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OfflinetaGyo
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22281382 - 09/23/15 09:41 PM (8 years, 7 months ago)

Gonna get these supplies tomorrow :thumbup:


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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: taGyo]
    #22281694 - 09/23/15 11:18 PM (8 years, 7 months ago)

Cornmeal for the win, taGyo! =)


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InvisibleFantastic Mr. Fox
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22281764 - 09/23/15 11:38 PM (8 years, 7 months ago)

Found it! lol
:tongue2:


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Fantastic Mr. Fox]
    #22282900 - 09/24/15 09:43 AM (8 years, 7 months ago)

Quote:

Fantastic Mr. Fox said:
Found it! lol
:tongue2:



:rockon:

On the way to the store now. Pickin' up some dab from a homie on the way :blazed:


--------------------
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InvisibleFantastic Mr. Fox
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: taGyo]
    #22282915 - 09/24/15 09:46 AM (8 years, 7 months ago)

Nice man, Nice!
Sounds like it's gonna be a good day,
:paradissunsmall:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Fantastic Mr. Fox]
    #22286078 - 09/24/15 09:02 PM (8 years, 7 months ago)

update on tamps and pan jamaica. tamps are finishing before pan jamaica. day 10 pan jamaica, day 4 p. tamps. pan jamaica finished about the same speed as the cubes on the cornmeal plate.


CORNMEAL MUSH PLATE LIQUID INOCULATION


Below is Side by Side Growth with 4 different cultures. Syzygy Clone 1 Cubensis, AA+ cubensis, Panaeolus (Copelandia) cyanescens : Jamaican, Psilocybe tampanensis

  • SYZYGY CLONE 1: Day 2-5


  • AA+ : Day 2-7


  • PAN. JAMAICA Day 2-10


  • P. TAMP. Day 2-5




--------------------
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:flame::chief:JOIN THE POW WOW:chief::flame:

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OfflinetaGyo
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22287306 - 09/25/15 07:07 AM (8 years, 7 months ago)

Gonna clean up some cultures today, got all my supplies, should be trying this in a week or two :thumbup:

Gonna do so with Golden Halo MS, AA+ MS, KSSS MS and hopefully Pink Buffalo and Red Boy MS.


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