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OfflineKizzle
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Sterilizing glass petris * 4
    #20563394 - 09/13/14 10:09 PM (7 years, 9 months ago)

I noticed there weren't any write-ups on sterilizing glass petris so I figured make one and hopefully squash some of the misconception about glass petris in the process.

To start here are dishes I'll be using. These are flint glass, no need to get the expensive borosilicate (pyrex) dishes. This material is pretty much the same as what mason jars are made of so as long you aren't pouring cool water on a hot dish or something they won't break. You can get them in all different sizes. These were just under $2 a piece. The lids fit loosely over base so they can open and closed quickly. Some have beaded edges to help keep the lid centered some don't. Each dish here weighs 40 grams so they're a bit heavier than the plastic ones.


Cleaning out the used dishes is extremely easy. I just scrape any leftover agar into the toilet. If the dishes were contaminated with mold place the still wrapped dish in the microwave and heat it, should only take 10-20 seconds, until the agar has completely liquified, let it resolidify and that should destroy most of the spores (actually destroyed all the spores in all the tests I've done) and trap what is left in the agar so you can clean it out without releasing them into the air.


Then wipe with paper towel firmly around the edges and good as new. It can be a bit of a pain if the agar is very old and has dried out so try to avoid that but otherwise it only take few seconds.


It's important especially if oven sterilizing to get all the agar off, don't forget the outside of the dish, or it'll leave little black patches of burned agar.

To oven sterilize pull out a large sheet of foil and stack the dishes in the center.


The foil should be wrapped as tightly as possible. Then grab another sheet and wrap it around, this time with the center of the foil over the top so the edges wind up on the bottom side. The edges of the foil should pressed together and folded tightly.

Set this on a rack, and place it in the oven. 1 hour at 450F should be sufficient but I usually leave it in for 2 because I don't have a lot of faith in Wikipedia:tongue: Be sure to let the dishes cool before removing from the oven. The wrapped dishes once cooled should be moved immediately into your SAB for use.


Another method of sterilizing them is to pressure cook them. It's quicker and doesn't require wasting large amounts of foil or having to awkwardly unwrap them under sterile condition. To do this find a polypropylene container, it'll have the PP5 code on the bottom and stack the dishes in. This is what I had on hand I can fit 4 dishes in each and it takes up about amount of PC space as a pint jar.


I already had a filtered hole in these containers from previous uses. I'm assuming a vacuum may form without it at least if they're sealed. The dishes are wrapped in foil over the filtered side and placed in a pressure cooker. These were sterilized for 1 hour at 10 psi.


To remove the sterilized dishes place the container upside down in the SAB and remove the bottom like so.


Now you have some sterile dishes to use. As a note condensation normally doesn't form on them during storage but as you can see it does when they've been recently poured.

My little in vitro clone here :lol:

I might add these are ideal for making spore prints or mixing spore solution. They can provide a sterile surface to set things on, like the cap to the agar bottle. The larger ones can be used to sterilize and store cotton swabs in.


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Edited by Kizzle (09/14/14 02:53 AM)


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Invisible36fuckin5
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Re: Sterilizing glass petris [Re: Kizzle]
    #20563420 - 09/13/14 10:13 PM (7 years, 9 months ago)

I would probably individually wrap them in foil then PC, but this looks like a decent method.


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Re: Sterilizing glass petris [Re: Kizzle]
    #20563424 - 09/13/14 10:14 PM (7 years, 9 months ago)

:takingnotes:


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Offlinekmetric
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Re: Sterilizing glass petris [Re: tripdawg420]
    #20563526 - 09/13/14 10:39 PM (7 years, 9 months ago)

I've heard some bad things about glass petris, have you had any problems with them Kizzle?

Seems to me more people would go for glass since you can reuse them..


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InvisiblecArcace-x
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Re: Sterilizing glass petris [Re: tripdawg420]
    #20563561 - 09/13/14 10:46 PM (7 years, 9 months ago)

:goodmorning: noted
i am considering using them
tired of throwing plastic petri in the garbage


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Re: Sterilizing glass petris [Re: kmetric]
    #20563571 - 09/13/14 10:47 PM (7 years, 9 months ago)

Good writeup, if I ever get them this will come in handy :thumbup:


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OfflineKizzle
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Re: Sterilizing glass petris [Re: kmetric]
    #20564260 - 09/14/14 02:13 AM (7 years, 9 months ago)

Quote:

kmetric said:
I've heard some bad things about glass petris, have you had any problems with them Kizzle?

Seems to me more people would go for glass since you can reuse them..



Only problem I can think of was a couple of them were cracked when they arrived in the mail. I'm not sure how, they were pretty well packed in bubble wrap. I haven't broken any while using them though and I've actually knocked one onto the floor couple times.

Also I have a feeling they wouldn't work well for no-pour but haven't tried it or anything.


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Re: Sterilizing glass petris [Re: Kizzle]
    #20564274 - 09/14/14 02:18 AM (7 years, 9 months ago)

:rockon:
nice stuff and about time we got a kizzle tek:cool:


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InvisiblePastywhyteMDiscord
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Re: Sterilizing glass petris [Re: Kizzle]
    #20564363 - 09/14/14 02:57 AM (7 years, 9 months ago)

Quote:

Kizzle said:
Also I have a feeling they wouldn't work well for no-pour but haven't tried it or anything.




Sarah Tonin seems to have had some luck with doing them no pour but maybe she's just lucky. How are they on the ergonomic end of things? I have heard mixed reviews but I would probably do a lot more no pour if I didn't have to deal with retarded shipping costs, waste, etc. Buying those and reusing would get me doing pour a lot more provided they weren't a total PITA to work with.


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Re: Sterilizing glass petris [Re: Pastywhyte]
    #20564380 - 09/14/14 03:05 AM (7 years, 9 months ago)

those look lendable...can i boorow some:begger::lol:


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OfflineKizzle
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Re: Sterilizing glass petris [Re: Pastywhyte]
    #20564489 - 09/14/14 04:20 AM (7 years, 9 months ago)

Compared to the improvised PP petris they're definitely easier to use and much easier to see through. Picking up these particular dishes took some getting used to. They're the old school kind where the bottom of the lids actually touch the rack they're sitting on. Supposedly it makes them less prone to contamination.


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Re: Sterilizing glass petris [Re: Kizzle]
    #20564499 - 09/14/14 04:26 AM (7 years, 9 months ago)

This is a good tek, what surface are you placing the petri dishes on upon removal from the pressure cooker? Are you using a flow hood or a glovebox? And when you pressure cook the dishes, are you including your agar mix?


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Re: Sterilizing glass petris [Re: starsky7]
    #20564500 - 09/14/14 04:28 AM (7 years, 9 months ago)

The reason I am asking is because in some environments, contamination is more likely to occur with long exposure to open air. I think it should be mentioned that the dishes should be moved to a still air environment, or a flow hood system as soon as possible.


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"In nature, there are neither rewards nor punishments, there are consequences" - Robert Greene Ingersoll


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OfflineKizzle
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Re: Sterilizing glass petris [Re: starsky7]
    #20564729 - 09/14/14 06:56 AM (7 years, 9 months ago)

I did mention it with the oven sterilizing. If using the PP containers though they should remain sterile indefinitely regardless of where the container is stored.

The agar is poured into them inside a still air box after sterilization. I usually do it while the agar is still pretty hot since it kills many (not all obviously) contaminants it may come in contact with so it's extra protection IMO. It does produce more condensation when pouring hot but it goes away eventually.

It's not absolutely necessary but I strongly recommend setting them on some kind of grille inside the box. It makes them easier to pick up and you can use it to keep them elevated above where contaminants are most likely to get stirred up while you're working.


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Re: Sterilizing glass petris [Re: Kizzle]
    #20564745 - 09/14/14 07:03 AM (7 years, 9 months ago)

Excellent. Helpful additions, thank you.


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InvisiblePastywhyteMDiscord
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Re: Sterilizing glass petris [Re: Kizzle]
    #20565843 - 09/14/14 01:42 PM (7 years, 9 months ago)

Quote:

Kizzle said:
Compared to the improvised PP petris they're definitely easier to use and much easier to see through. Picking up these particular dishes took some getting used to. They're the old school kind where the bottom of the lids actually touch the rack they're sitting on. Supposedly it makes them less prone to contamination.




Great stuff kizzle thanks :thumbup:


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Re: Sterilizing glass petris [Re: 36fuckin5]
    #20565952 - 09/14/14 02:12 PM (7 years, 9 months ago)

Quote:

36fuckin5 said:
I would probably individually wrap them in foil then PC, but this looks like a decent method.



:whathesaid:

I didn't even individually wrap mine. I have 48 pyrex dishes. I would just wrap 10 of them stacked in foil and PC them for 20-30M. I just hate using glass dishes compared to the polystyrene ones. Even with my nice pyrex dishes I got for free I still by the disposables due to the ease of use.



definitely not worth the money unless you can find them for free


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OfflineKizzle
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Re: Sterilizing glass petris [Re: bodhisatta]
    #20568153 - 09/14/14 09:52 PM (7 years, 9 months ago)

I did start off wrapping them individually but it really was a huge pain in the ass compared to bundling them together. These methods take only a couple minutes of actual effort. Not that I have anything against the disposable ones. If I had some I would use them instead I just hate having to worry about running out or having to keep ordering stuff. I'm also the kind klutz who could ruin a whole stack petris just by doing something stupid, something I don't have to worry about now. I if I do something I think may have contaminated I just toss it aside.


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Re: Sterilizing glass petris [Re: Kizzle]
    #20568414 - 09/14/14 10:39 PM (7 years, 9 months ago)

great SHIT kizzle, The PP5 containers are the way i do mine !!!

Cyn


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OfflineKizzle
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Re: Sterilizing glass petris [Re: TheCyndicate]
    #20608201 - 09/23/14 09:43 PM (7 years, 8 months ago)

I think in the near future I'm going to try the oven sterilization with glass jars instead of foil like the way the PP containers are used in the PC method. I have a couple glass pint jars I think the smaller dishes will fit into.


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