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yes, the spores in the needle will be killed but that is only a small amount of spores compared to what you inject into the jar. if you kill the ones in the needle and then inject 1 or 2 ccs of solution you will still have plenty of non-dead spores in the jar. no real way to get around it. if you want to kill the non-spore things that might be in the needle, you will have to kill some spores also.