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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
GETTING WORRIED....NEED HELP
    #9939210 - 03/09/09 11:59 AM (15 years, 10 months ago)

My jars have been inoculated now for 9 nine days and there is still is no sign of mycelium growing. I followed the PF Tek completely and cant figure out whats going on. BRF substrate jars were boiled for 90 mins with the water half-way up the jars with a washcloth under them as to not transfer too much heat. I let them sit overnight and inoculated them the next morning in a plastic enclosed cupboard that was sprayed with anitbacterial spray and the needle of the syringe was sterilized between each jar. They have since been stored in the dark at 80-86 degrees and nothing has happened yet. I find it hard to believe the spores were bad because I used 3 different syringes and knocked the jars up in thirds with B+, Burma, and Golden Teachers. Neither set of 3 has any signs of mycelium. Does anybody know what could be going on here.

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Offlineethicdrummer
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9939222 - 03/09/09 12:03 PM (15 years, 10 months ago)

well a couple problems here: for one you should have steamed them with the water level just below the bottom of the jars not touching. and the biggy here is your storing them wayyyyy too hot. room temp friend is all you need. and when you say you sterilized between jars i hope you mean with a flame, and not just alchohol. Let's see what others say, But definitely lower those temps!

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InvisibleHorusTh3Chrous


Registered: 11/14/05
Posts: 955
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9939232 - 03/09/09 12:05 PM (15 years, 10 months ago)

it takes 3 weeks.

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Invisibledjmako7
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Registered: 06/13/06
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9939235 - 03/09/09 12:06 PM (15 years, 10 months ago)

Quote:

brandonb1369 said:
My jars have been inoculated now for 9 nine days and there is still is no sign of mycelium growing. I followed the PF Tek completely and cant figure out whats going on. BRF substrate jars were boiled for 90 mins with the water half-way up the jars with a washcloth under them as to not transfer too much heat. I let them sit overnight and inoculated them the next morning in a plastic enclosed cupboard that was sprayed with anitbacterial spray and the needle of the syringe was sterilized between each jar. They have since been stored in the dark at 80-86 degrees and nothing has happened yet. I find it hard to believe the spores were bad because I used 3 different syringes and knocked the jars up in thirds with B+, Burma, and Golden Teachers. Neither set of 3 has any signs of mycelium. Does anybody know what could be going on here.




Well I can first start by saying You did not follow the correct measures of the PF Tek. The water is not suppose to touch the jars. The steam from the water is used to sterilize not the water.
No need to go above 80 degrees during incubation. Going higher than that has been known to slow growth and an ideal breeding ground for contamination.

Wait another week. Sometimes the strains may be a slow colonizer.


--------------------



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OfflineAlex TrebekS
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9939551 - 03/09/09 01:11 PM (15 years, 10 months ago)

Do a few test runs with the pot.


Try to figure out how much water you need to keep steam going for 90minutes

Then use something to prop those jar up out of that water. Use anything ..
If you have nothing, make something. I've personally seen somebody take empty soda cans rinsed out that were placed in standing up with aluminum foil over them for a base


Jars are a few degree's warmer then the temperature around them.  You don't want them going over 80 degree's

I put mine in a bookshelf in a bedroom that is heated to 70degree's. (rest of my house is at 60, I'm cheap)

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OfflineAlex TrebekS
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9939563 - 03/09/09 01:15 PM (15 years, 10 months ago)

ohh yea they don't need to be in the dark for colonization

everybody suggest put them in indirect light from day 1

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OfflineDespondent
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Re: GETTING WORRIED....NEED HELP [Re: Alex Trebek]
    #9939709 - 03/09/09 01:48 PM (15 years, 10 months ago)

When u sterilize with ur jars in water alot of moisture tends to get in them even with aluminum foil over them. Set the out of the water. I use a few layers of jar lids seperated by foil. When ur incubating u want ur temps around 73 degrees F. Then be patient. you should see some growth after about a week or so.

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OfflineBubonic
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Re: GETTING WORRIED....NEED HELP [Re: Despondent]
    #9939871 - 03/09/09 02:15 PM (15 years, 10 months ago)

From day 3 of my last grow project only half my jars started growing, even though all the jars were from the same syringe.  It took about 2 weeks before the other half showed any signs of growth.  So I'd say don't panic yet, give em' at least two weeks or so before you start getting too worried, sometimes they just take a while.  Id say don't let the temp get over 82 if your gonna incubate, I just inoculated another batch three days ago, 2 separate strains, incubated @ 82 degrees and got my first signs of growth from both today.  I've let water touch my jars during sterilization (in a slow cooker on high for 90 min) every time I grow, and have had abosolutly no problems thus far.


--------------------
Ring around the rosy
A pocketful of posies
Ashes, Ashes
We all fall down!

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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
Re: GETTING WORRIED....NEED HELP [Re: Bubonic]
    #9945537 - 03/10/09 12:32 PM (15 years, 10 months ago)

it seems now that patience was the key here.....i just noticed two small patches of mycelium about 1 cm in diameter starting to grow in one of the jars. i just hope that the other 11 jars start to do the same

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InvisibleStarrider
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Registered: 11/09/08
Posts: 499
Loc: Between here and there
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9945571 - 03/10/09 12:40 PM (15 years, 10 months ago)

I followed the PF Tek to the letter and it took my PT jars a month to show growth in them. Growing shrooms takes a lot of patience. Go slow and you will find that it is worth the effort.


--------------------

"If the doors of perception were cleansed every thing would appear to man at it is, infinite."

William Blake


Anything that I write is for fun only. I am creating fiction for the pleasure of my readers.

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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9951908 - 03/11/09 01:59 PM (15 years, 10 months ago)

is this what i should be seeing after 11 days?

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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9960760 - 03/12/09 07:52 PM (15 years, 10 months ago)

I have another question to go along with my grow. Since the cakes are taking so long to start colonizing, should I wait longer than 1 week to birth after 100% colonization? Im wondering since usually you only have to wait 1 week after for cakes that start colonizing within a few days

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OfflineSethTheGreat
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Registered: 01/21/09
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9960822 - 03/12/09 08:01 PM (15 years, 10 months ago)

Quote:


I have another question to go along with my grow. Since the cakes are taking so long to start colonizing, should I wait longer than 1 week to birth after 100% colonization? Im wondering since usually you only have to wait 1 week after for cakes that start colonizing within a few days



both times now I have waited for pins.
First time took 4 days after the full
Second time took exact 7 days,

after pins then take her out.
Dunk an roll with em on
and if they come off...... oh well you got yourself a quick snack
(don't underestimate those little bastards, they can make youtrip)


--------------------
HazeTiger SharkKushBuddah

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OfflineSethTheGreat
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Re: GETTING WORRIED....NEED HELP [Re: SethTheGreat]
    #9960855 - 03/12/09 08:07 PM (15 years, 10 months ago)

Quote:

is this what i should be seeing after 11 days?




lookin good my man,

***Advice***
fresh air makes mushies grow(as well as 100 percentage), keep that bad  boy in a cupboard and try not let the stale air lose(in other words keep it closed).
Also i know its damn near impossible, but let it be, colonization will be quicker(at least it'll seem like it:))


I had a jar that I checked like 4 times a day, It started growing mushrooms before half colonization, created a real dilema.
Could of been a number of things but what i gathered...it was the air.
good luck dude, you'll prolly have the ingredients for a good trip soon.


--------------------
HazeTiger SharkKushBuddah

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Offlinephishie
whistling moon travler
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Registered: 05/13/08
Posts: 470
Last seen: 13 years, 7 months
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9960945 - 03/12/09 08:21 PM (15 years, 10 months ago)

Where did you get your spores???? this is 8 days growth I'm now at like 12 or 13 days and fully colonized... I had problems the first 2 times I attempted to grow. pretty sure it was the sporestores bad spores that did my first 2 attempts in. all I have to say is shop with shroomery sponsors and ask alot of questions.


--------------------
VVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVV



Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin while in the USA.


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Offlinephishie
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Registered: 05/13/08
Posts: 470
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Re: GETTING WORRIED....NEED HELP [Re: phishie]
    #9960965 - 03/12/09 08:24 PM (15 years, 10 months ago)

not a professional but shouldn't he take that tinfoil off the top of that jar??!! correct me if I'm wrong but I did that the first time I tryed to grow and was not good for me.


--------------------
VVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVVV



Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin while in the USA.


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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
Re: GETTING WORRIED....NEED HELP [Re: phishie]
    #9961011 - 03/12/09 08:32 PM (15 years, 10 months ago)

Quote:

phishie said:
Where did you get your spores???? this is 8 days growth I'm now at like 12 or 13 days and fully colonized... I had problems the first 2 times I attempted to grow. pretty sure it was the sporestores bad spores that did my first 2 attempts in. all I have to say is shop with shroomery sponsors and ask alot of questions.






I didnt do my research of vendors before I made my purchase.....I went the cheaper way and bought from SporeStore (should've went with a Shroomery vendor). It's taken a while now but almost all of the jars are showing signs of mycelium. I think the slow colonization was because the fine verm may have been too compacted in the substrate along with the fact that I had the foil crimped tightly around the lid of the jar making it near air tight. I have since loosened the foil as well as the lids a little to allow for air exchange and thats when mycelium started showing up in the rest of the jars.

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OfflineOatman2000
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Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9961080 - 03/12/09 08:44 PM (15 years, 10 months ago)

your cakes are wet. Probably water that spilled in the holes while PC'n or hopefully not Boiling... (you can get away with it.)

I've colonized wet cakes that looked like that before. I've also seen some fail too. Good luck to ya laddie. 


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine

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Offlinebrandonb1369
Fungi Farmer


Registered: 03/09/09
Posts: 107
Last seen: 11 years, 9 days
Re: GETTING WORRIED....NEED HELP [Re: Oatman2000]
    #9965078 - 03/13/09 02:02 PM (15 years, 10 months ago)

Now that I think about it...the moisture could def be a factor in the slow colonization. When I mixed up the substrate, it seemed like it was very dry and I used 8 cups verm: 3 cups BRF: 3 cups water. I figured that it shouldnt have been so dry so I added about another cup of water, thinking it would be better. Looking back now, I shouldve just stuck to the recipe. I just mixed up some more substrate to make a cake in a 2 cup container. I used 1 1/3 cup verm, 1/2 cup BRF and 1/2 cup of water. It was a lot easier to work with than the prior batch (easier to fill container and level out). I have the container sterilizing right now and Sunday when I get back into town, I'll 'noc it up the same way I did the others and see if it starts growing mycelium sooner than my other jars did. At least then I'll know that the water content was my problem in the first place.

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InvisibleMoniker_Mike
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Registered: 02/06/09
Posts: 105
Loc: New England
Re: GETTING WORRIED....NEED HELP [Re: brandonb1369]
    #9965113 - 03/13/09 02:10 PM (15 years, 10 months ago)

You still have your ratios off. It's 1 cup verm to 1/2 cup of water and 1/2 cup of BRF. Make sure you mix your verm and water before putting in your BRF, this ensures that the BRF will properly coat the pieces of vermiculite.

Goodluck Mate.


--------------------
I have a theory that the truth is never told during the nine-to-five hours. - HST



MONIKER COLLECTIVE SHIRTS AVAILABLE VIA MYSOTI.COM

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