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OfflineArthurSalenez
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Does this happen to anyone?
    #9911522 - 03/04/09 04:16 PM (15 years, 18 days ago)

I used to syringe that I bought from a friend, said he took some spores from two mushroom caps and made it into one full shot. I inoculated 6 jars and only two came out developing mycelium. Anyone have a clue?

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9911548 - 03/04/09 04:20 PM (15 years, 18 days ago)

wow too much info.. sure everyones had some jars grow slower than others.. the spore solution I'm using is made up of two prints from my B+ grow. works fine two prints to 1pint of water


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9912013 - 03/04/09 05:32 PM (15 years, 18 days ago)

I have the penis envy spore syringe.

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912133 - 03/04/09 05:52 PM (15 years, 18 days ago)

how about u add more info like how long its been? how much solution u used?


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9912293 - 03/04/09 06:30 PM (15 years, 18 days ago)

Quote:

madwire3 said:
how about u add more info like how long its been? how much solution u used?




Wow, testy testy!

It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912309 - 03/04/09 06:32 PM (15 years, 18 days ago)

not testy just can't help when u are so breif... again more info how long has it been since u inoculated


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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Offlinelittleapplemm
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912414 - 03/04/09 06:56 PM (15 years, 18 days ago)

Quote:

ArthurSalenez said:
It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.




2 cc's left of a 10 or 12 cc syringe? That seems like a lot of inoculant per jar to me...


--------------------
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Invisibleshroomzey
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Re: Does this happen to anyone? [Re: littleapplemm]
    #9912568 - 03/04/09 07:26 PM (15 years, 18 days ago)

You need to describe your entire process.  What did you inooculate?  What size jars?  How did you sterilize them?  For how long? 

It's like coming to a doctor and saying," I hurt."  And thats it.

He can't do anything until you tell him what happened, where, what you hit, all the details.


--------------------

200 years from now, we will look back and laugh at ourselves for how stupid we were.
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Find a respected member of the community and study them.  I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: shroomzey]
    #9914378 - 03/04/09 11:51 PM (15 years, 18 days ago)

Quote:

shroomzey said:
You need to describe your entire process.  What did you inooculate?  What size jars?  How did you sterilize them?  For how long? 

It's like coming to a doctor and saying," I hurt."  And thats it.

He can't do anything until you tell him what happened, where, what you hit, all the details.




Lol, I hurt. lol

I don't expect a diagnosis. However, I wanted to ask a simple question that relied upon simple answers. Like a yes or no. You all are dodging it.

My original question was: Does this happen to anyone?

Rephrasing: Does this happen to anyone on a regular basis during cultivation?

But if you dying to know the exact details:

pf Tek jars
I inoculated jars
I Boiled them for 1 hour


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: littleapplemm]
    #9914413 - 03/04/09 11:58 PM (15 years, 18 days ago)

Quote:

littleapplemm said:
Quote:

ArthurSalenez said:
It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.




2 cc's left of a 10 or 12 cc syringe? That seems like a lot of inoculant per jar to me...




I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914418 - 03/05/09 12:00 AM (15 years, 18 days ago)

haha k my answer is blue...  good luck being a ass


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineMyName
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914423 - 03/05/09 12:00 AM (15 years, 18 days ago)

Okay is that in order?  you inoculated then boiled?  Think you just told us what went wrong.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9914429 - 03/05/09 12:02 AM (15 years, 18 days ago)

Quote:

madwire3 said:
haha k my answer is blue...  good luck being a ass




No problem, butch. :cool:

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: MyName]
    #9914436 - 03/05/09 12:03 AM (15 years, 18 days ago)

no no don't try to break down what's wrong he doesn't want help


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: MyName]
    #9914451 - 03/05/09 12:06 AM (15 years, 18 days ago)

Quote:

MyName said:
Okay is that in order?  you inoculated then boiled?  Think you just told us what went wrong.




Of course not, i boiled then inoculated. But this topic is not about what went wrong, but why mycelium is only developing in 2 out of 6 jars.

Possibilities:

The more spores the faster the mycelium growth.

Or other unknown reasons.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9914457 - 03/05/09 12:07 AM (15 years, 18 days ago)

Quote:

madwire3 said:
no no don't try to break down what's wrong he doesn't want help




Please leave.

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914462 - 03/05/09 12:08 AM (15 years, 18 days ago)

Quote:

ArthurSalenez said:

I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.




1/4 of 1CC? Thats not good, should have atleast 1CC for each inoculation point. I inoculated 3/4 holes on one of my jars with uneven amounts and the last hole got less than 1CC and hasn't shown any signs of growth from that point. I inoculated on 02/22, all other jars are 90% colonized.


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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914467 - 03/05/09 12:10 AM (15 years, 18 days ago)

u started it I was trying to help but what u don't get is in order to figure out why theres no myc people need some info u got butt hurt when I was trying to help. act like that and no one will help u here


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: madwire3]
    #9914479 - 03/05/09 12:12 AM (15 years, 18 days ago)

Quote:

madwire3 said:
u started it I was trying to help but what u don't get is in order to figure out why theres no myc people need some info u got butt hurt when I was trying to help. act like that and no one will help u here




True dat.


--------------------

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914482 - 03/05/09 12:13 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:

I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.




1/4 of 1CC? Thats not good, should have atleast 1CC for each inoculation point. I inoculated 3/4 holes on one of my jars with uneven amounts and the last hole got less than 1CC and hasn't shown any signs of growth from that point. I inoculated on 02/22, all other jars are 90% colonized.




I was afraid that introducing more liquid into the jar would stimulate bacterial growth, according to what another forum member told me.
Would it be okay to use up the remaining 2 ccs on just one jar? Or is that too much? Thanks for understanding the question.

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Offlinetr8orjohn
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Re: Does this happen to anyone? [Re: madwire3]
    #9914489 - 03/05/09 12:15 AM (15 years, 18 days ago)

Simple answer: Yes, that happens.

Why people are asking questions is because most likely there is a *reason* it happened. Whats "slow" to you? Are there *no* signs of growth, or is it just being slow (stalling)? Did you shake the syringe properly?

More spore water does not *necessarily* mean more growth kids...and can actually mean slower growth. I have always heard (and do myself) noc two out of four ports with 0.5 cc each. Then again, I can't seem to get my cakes to give me more then one flush :mad2:

Bickering isn't going to get anyone anywhere

-jon


--------------------
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Terry M said:
Looking for rules that other people use successfully. Sterile procedure is just a set of rules that work.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9914491 - 03/05/09 12:16 AM (15 years, 18 days ago)

Quote:

madwire3 said:
u started it I was trying to help but what u don't get is in order to figure out why theres no myc people need some info u got butt hurt when I was trying to help. act like that and no one will help u here




The only thing I started was the topic. You asked me questions that had no reference to the topic question. If you like to waste my time then that's okay, type whatever you want. I don't care. I'm not here to make friends, I have plenty of shit to do in the real world.

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914492 - 03/05/09 12:16 AM (15 years, 18 days ago)

Quote:

ArthurSalenez said:

I was afraid that introducing more liquid into the jar would stimulate bacterial growth, according to what another forum member told me.
Would it be okay to use up the remaining 2 ccs on just one jar? Or is that too much? Thanks for understanding the question.




Wouldn't be too much, shoot up 2 points, but it'll take some time. I fucked up badly, I used 3 syringes for 6 jars.. That's too much. It's my first grow and 5/6 jars are 90% colonized w/ no contams.


--------------------

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Offlinemadwire3
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914499 - 03/05/09 12:18 AM (15 years, 18 days ago)

who said I wanted to be ur friend? u camehere for a answer that's what was being done.. but whateva grumpy


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: tr8orjohn]
    #9914502 - 03/05/09 12:19 AM (15 years, 18 days ago)

Quote:

tr8orjohn said:
Simple answer: Yes, that happens.

Why people are asking questions is because most likely there is a *reason* it happened. Whats "slow" to you? Are there *no* signs of growth, or is it just being slow (stalling)? Did you shake the syringe properly?

More spore water does not *necessarily* mean more growth kids...and can actually mean slower growth. I have always heard (and do myself) noc two out of four ports with 0.5 cc each. Then again, I can't seem to get my cakes to give me more then one flush :mad2:

Bickering isn't going to get anyone anywhere

-jon




I understand why people are asking me questions. I appreciate it.

But that doesn't explain my question.

Thanks for the information.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9914511 - 03/05/09 12:26 AM (15 years, 18 days ago)

Quote:

madwire3 said:
who said I wanted to be ur friend? u camehere for a answer that's what was being done.. but whateva grumpy




That's fine. Good day mate.

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914520 - 03/05/09 12:29 AM (15 years, 18 days ago)

Here's a pic of the jar that recieved less than 1CC of solution. The area inbetween the two sides in the inoculation point(no growth). To answer your question, yes it happens to those who stray from the Tek. Follow the derections in RR's videos and you'll be fine.



--------------------

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914532 - 03/05/09 12:33 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:

I was afraid that introducing more liquid into the jar would stimulate bacterial growth, according to what another forum member told me.
Would it be okay to use up the remaining 2 ccs on just one jar? Or is that too much? Thanks for understanding the question.




Wouldn't be too much, shoot up 2 points, but it'll take some time. I fucked up badly, I used 3 syringes for 6 jars.. That's too much. It's my first grow and 5/6 jars are 90% colonized w/ no contams.




Could it be possible to avoid contaminates by lowering the temperature of a room or placing it in a customized refrigerator? My last batch got contaminated around the 90% growth mark, just when it was fully colonized the bottom reeked of a brown agent. Even when I got to cutting my cakes in half it seems as if the contamination was internal. It surprised me when I used extra precautions by baking the verm and brown rice flour beforehand and boiled the jars for 2 hours. I've even read a growing journal from another forum where a member was talking about sterilization in culture jars, declaring that boiling does not kill all contaminants.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914546 - 03/05/09 12:37 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Here's a pic of the jar that recieved less than 1CC of solution. The area inbetween the two sides in the inoculation point(no growth). To answer your question, yes it happens to those who stray from the Tek. Follow the derections in RR's videos and you'll be fine.




Thanks, the RR videos were really helpful. How long has it been since inoculation in the photo?

Edited by ArthurSalenez (03/05/09 12:40 AM)

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914553 - 03/05/09 12:38 AM (15 years, 18 days ago)

Quote:

ArthurSalenez said:
Could it be possible to avoid contaminates by lowering the temperature of a room or placing it in a customized refrigerator? My last batch got contaminated around the 90% growth mark, just when it was fully colonized the bottom reeked of a brown agent. Even when I got to cutting my cakes in half it seems as if the contamination was internal. It surprised me when I used extra precautions by baking the verm and brown rice flour beforehand and boiled the jars for 2 hours. I've even read a growing journal from another forum where a member was talking about sterilization in culture jars, declaring that boiling does not kill all contaminants.




If you add the 1 inch of dry vermiculite to the tops of the 1/2 pint jars it should keep out contams. It's used as the contamination barrier. As you see in my pics I added the coffee filter to the top, which may or may not benefit against contams, but it keeps me sound asleep at night. I never baked the verm or brown rice flour, I simply steam sterilized for about an hour and a half, +5 mins, to ensure the times I came to check on it were made up as well.


--------------------

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914569 - 03/05/09 12:43 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:
Could it be possible to avoid contaminates by lowering the temperature of a room or placing it in a customized refrigerator? My last batch got contaminated around the 90% growth mark, just when it was fully colonized the bottom reeked of a brown agent. Even when I got to cutting my cakes in half it seems as if the contamination was internal. It surprised me when I used extra precautions by baking the verm and brown rice flour beforehand and boiled the jars for 2 hours. I've even read a growing journal from another forum where a member was talking about sterilization in culture jars, declaring that boiling does not kill all contaminants.




If you add the 1 inch of dry vermiculite to the tops of the 1/2 pint jars it should keep out contams. It's used as the contamination barrier. As you see in my pics I added the coffee filter to the top, which may or may not benefit against contams, but it keeps me sound asleep at night. I never baked the verm or brown rice flour, I simply steam sterilized for about an hour and a half, +5 mins, to ensure the times I came to check on it were made up as well.




Thanks. How old is the mycelium in the photo prior to you injecting the spores into the jars? Was your syringe home made or bought from a vendor?

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914577 - 03/05/09 12:44 AM (15 years, 18 days ago)

Home made syringes from a friend, I inoculated on 02/22. About 11 days.


--------------------

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914594 - 03/05/09 12:51 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Home made syringes from a friend, I inoculated on 02/22. About 11 days.




Aw, I guess that's my problem. The vendors gave me not a whole lot of spores, and considering that PE spores are hard to obtain. Is there an official and reliable website about the Penis envy strain?

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914599 - 03/05/09 12:52 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Home made syringes from a friend, I inoculated on 02/22. About 11 days.




One last question, are your syringe needles nail thin or microscopy size?

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914600 - 03/05/09 12:52 AM (15 years, 18 days ago)

Any of the Shroomery Sponsors, my strain came from RalphstersSpores.


--------------------

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914603 - 03/05/09 12:54 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Any of the Shroomery Sponsors, my strain came from RalphstersSpores.




Thanks for being patient with me. 5 Mushies.

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OfflineMuffinlad
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914604 - 03/05/09 12:54 AM (15 years, 18 days ago)

Quote:

ArthurSalenez said:

One last question, are your syringe needles nail thin or microscopy size?




All 3 of my syringes look needle thin to me. I don't think they're microscopic because I'm not using a microscope to see them :]


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914620 - 03/05/09 01:01 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:

One last question, are your syringe needles nail thin or microscopy size?




All 3 of my syringes look needle thin to me. I don't think they're microscopic because I'm not using a microscope to see them :]




The spores from Sporeworks.com throw in some microscopy items (like a small rectangular glass sheet with some super thick needles; they even throw in a free syringe if you mention the shroomery in the comment box) I guess that works for all vendors. But I finally noticed that spores tend to clog up small needles, that's why I wasted about 1 cc off a 10 CC shot. I squirted some of the liquid on to a nearby wall during inoculation. Sucks, the syringe alone cost me 25+ bucks.

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OfflineMuffinlad
the hippie

Registered: 12/19/08
Posts: 110
Last seen: 12 years, 1 month
Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914631 - 03/05/09 01:04 AM (15 years, 18 days ago)

Quote:

ArthurSalenez said:
The spores from Sporeworks.com throw in some microscopy items (like a small rectangular glass sheet with some super thick needles; they even throw in a free syringe if you mention the shroomery in the comment box) I guess that works for all vendors. But I finally noticed that spores tend to clog up small needles, that's why I wasted about 1 cc off a 10 CC shot. I squirted some of the liquid on to a nearby wall during inoculation. Sucks, the syringe alone cost me 25+ bucks.




It beats wasting 3 syringes for 6 jars any day. I figured I don't know how to read a syringe.


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OfflineArthurSalenez
Golden Block Of Bud
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Registered: 10/23/08
Posts: 551
Last seen: 14 years, 1 month
Re: Does this happen to anyone? [Re: Muffinlad]
    #9914646 - 03/05/09 01:06 AM (15 years, 18 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:
The spores from Sporeworks.com throw in some microscopy items (like a small rectangular glass sheet with some super thick needles; they even throw in a free syringe if you mention the shroomery in the comment box) I guess that works for all vendors. But I finally noticed that spores tend to clog up small needles, that's why I wasted about 1 cc off a 10 CC shot. I squirted some of the liquid on to a nearby wall during inoculation. Sucks, the syringe alone cost me 25+ bucks.




It beats wasting 3 syringes for 6 jars any day. I figured I don't know how to read a syringe.




Lol, I don't think any of the syringe providers intended on making shots just for the sake of injecting mushroom spores into a cookie jar lol

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Offlinedead
grateful
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Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914955 - 03/05/09 04:38 AM (15 years, 17 days ago)

1cc is plenty for one jar. It is actually enough even for a quart jar of grain, so using more than 1cc for a half pint cake is just wasting inoculant and does not benefit you in any way. Quite the opposite, using too many spores in a substrate will only bring you smaller yields.

If you experience slower colonization when you use less spore solution, the problem is somewhere else, maybe your substrate is too dry or too wet, or doesn't get enough gas exchange... hard to say. It could just be the randomness of multispore inoculations.

Another thing, clear syringes are the best. You don't need to see any spores in the syringe. Use a minimal amount of spores for best results.


--------------------
"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."

Links:
:regularshroom:Nibin's Guide for Noobs
:regularshroom:some easy teks on bulk & grain prep. (my journal)

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