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kingjames488
Stranger
Registered: 02/18/09
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staying self-sufficient
#9905008 - 03/03/09 05:25 PM (15 years, 1 month ago) |
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hi all
Whats the best way for a beginner to become self-sufficient at growing mushrooms.
I mean so you dont have to keep buying syringes every time you want to grow some mushrooms... unless you want to try a new strain or something.
seems like the easiest way is spore prints, but it sounds like a bit of a pain to inoculate things with them.
so whats the easiest way to keep yourself going?
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Cascadian
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Spore prints are easy as fuck.
Tissue cultures require you to buy agar and petri dishes (or use the bottom of 1/2pint jars) AND make up some kind of glove box. They are also far easier than people seem to think, however.
Research cloning.
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Enothe
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Re: staying self-sufficient [Re: Cascadian]
#9905327 - 03/03/09 06:08 PM (15 years, 1 month ago) |
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look into the light corn syrup Liquid Culture tek.LC basics
this lets you clone a mushroom, so pick a big one that blues Alot when you cut it. get one with good genetics and you can get some super strong shroomies!
just make the LC, then fill a clean syringe with some and innoc your jars with that syringe, rinse and reuse.
LC can be done very easily. stock up on some syringes and then just reuse them. i also made some lids with a silicone plug for easy and clean syringe filling.
spore printes are super easy but easy to get infected too =/ id make a bunch just incase all else fails.
-------------------- "Never Knows Best"
Edited by Enothe (03/03/09 06:13 PM)
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kingjames488
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Re: staying self-sufficient [Re: Enothe]
#9905803 - 03/03/09 07:04 PM (15 years, 1 month ago) |
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taking spore prints looks easy enough, it's using them that sounds problematic. (dry spores, contams, etc.)
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Killa420
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Its not hard to scrape some spores in a glass. Squirt your sterile water in, suck back up, inject your jars. You shouldnt have any contams if took the print properly
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Peterthinks
(Caulking) gun for hire
Registered: 11/10/04
Posts: 2,379
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shoot sterile water into a grown cake and suck it out again. do this just before you birth it.
instant liquid culture ready to start the next batch.
You can wrap a syringe in foil and sterilize it again in the PC, just make sure you have couple CCs of water in it and a couple CCs of air.
Take prints of your mushrooms. make a little wire rack and put the cap on it above a jar of sterile water. let it sit under a bowl for a day so it drops spores into the water.
use what you got.
You don't have to make it any more complicated than it has to be.
some jars a torch or alcohol lamp brown rice flour vermiculite perlite Tyvec A tray A spore syringe A small PC A mister some aluminum foil micropore tape and a shotgun fruiting chamber.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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fltdriver82
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Re: staying self-sufficient [Re: Killa420]
#9905938 - 03/03/09 07:21 PM (15 years, 1 month ago) |
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You could always do WBS, spawn to bulk-reserving 1/5th of jar for LC: Add distilled water, swirl, withdraw myc water. Just made +200cc of both PF Redspore and Golden Teacher while I was spawning- extra bonuses are always great.
-------------------- The Fourth Amendment gives every American the right to refuse any search without a warrant. Say hello to my friend, his name is George. Every other ? you have is answered here In soviet russia, mushroom trips on you
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desoul420
RAVER
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Re: staying self-sufficient [Re: fltdriver82]
#9906065 - 03/03/09 07:37 PM (15 years, 1 month ago) |
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Great Topic=))
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PreparationH
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Re: staying self-sufficient [Re: desoul420]
#9906307 - 03/03/09 08:06 PM (15 years, 1 month ago) |
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you can use mushroom tissue in an LC? or did i misunderstand that? If you mean by using its spores to make the LC isnt that multispore?
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Vaelu
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Quote:
PreparationH said: you can use mushroom tissue in an LC? or did i misunderstand that? If you mean by using its spores to make the LC isnt that multispore?
I'm pretty sure you jab a mushroom stem and inject it into karo/water mixture with a syringe. That becomes a LC of the shroom you want to clone.... I think
-------------------- "When the student is ready the teacher appears..."
Edited by Vaelu (03/03/09 08:13 PM)
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PreparationH
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i can see this working but if it does why bother with agar?
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Cascadian
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Youve been here for almost four years and didnt know you could use mushroom tissue to make a liquid culture?
Regardless, agar is good because you can see contaminants. with a LC they might not be obvious until you use it to inoculate some jars.
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PreparationH
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nope i been here 4 years just started growing within the last 6 months lol. I figured you could because i know you can use mushroom tissue on agar. I'm gonna just try and clone with lc next now.
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kingjames488
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i think one of the main reasons to use agar is to isolate good sections of colonization for better results... not sure tho
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Peterthinks
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Registered: 11/10/04
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Agar work is to clean dirty cultures or prints, start clones isolate strains and test liquid cultures.
grow it, cut out a good looking clean part, grow that, cut the best part of that and use the culture from there.
Agar is important but not everyone needs to bother with it. When buddy sends you a print on the back of a dirty napkin from a Cambodian jungle cafe you begin to consider agar work as something you may wish to invest in. When you get that foot tall shroom that pushes your FC lid off in 1 day you begin to consider agar work. when one jar colonizes in 4 days and eats green mold while doing it you begin to consider agar work.
multi spore is a good way to go but you can really stack the deck in your favor by working with agar....weeds out those couch potato parts of the strain and concentrates the "I WANNA EAT AND FRUIT NOW!!!" parts.
Every strain that has a name owes it's existence to agar work and some crazy dude in front of a flow hood or with his arms jammed in a box.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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PreparationH
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yea but if i get a good fruit and clone it in liquid culture i'd have the same thing as working from agar and knocking up a jar with the wedge correct?
Edited by PreparationH (03/03/09 08:38 PM)
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littleapplemm
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I do believe you are correct, Prep H.
-------------------- Taco Bell tastes better with bike grease! ---------------------------------------------- See what projects I'm working on here
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PreparationH
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either way i want to get into agar after i perfect actually growing lol. I just started a few lc's for quicker colonization and have 20 rye quarts ready to fruit
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Peterthinks
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Registered: 11/10/04
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when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
when you work from spores on agar you isolate "sectors" or wedges of distinct growth that radiate out from a point.
It is unknown what these spore sectors will do unless you fruit them.
You could just chuck a bit of inner tissue from a mushroom into liquid culture and make a clone that way. But it is almost impossible to see contamination in a liquid culture. You better have supreme confidence in your sterile work before you knock up 20 quarts of grain with an untested LC.
Just use whatever you're cozy with. Or at least do a test grain jar.
LC to grain Wedge to wild bird seed spores to spawn bag It doesn't matter what you do or how you do it just keep it clean.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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PreparationH
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thanks for the explination thats +5 in my book.
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RedDevil420
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also, agar to grain has less chance of contams when done correctly compared to LC, which is supposed to be more contam prone.
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
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The shroomy 1
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Re: staying self-sufficient [Re: Peterthinks]
#9907759 - 03/03/09 11:24 PM (15 years, 1 month ago) |
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Quote:
Peterthinks said: when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
when you work from spores on agar you isolate "sectors" or wedges of distinct growth that radiate out from a point.
It is unknown what these spore sectors will do unless you fruit them.
You could just chuck a bit of inner tissue from a mushroom into liquid culture and make a clone that way. But it is almost impossible to see contamination in a liquid culture. You better have supreme confidence in your sterile work before you knock up 20 quarts of grain with an untested LC.
Just use whatever you're cozy with. Or at least do a test grain jar.
LC to grain Wedge to wild bird seed spores to spawn bag It doesn't matter what you do or how you do it just keep it clean.
Quote:
when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
That is confusing. Please read what you just said... You contradict yourself by saying Quote:
when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
-------------------- AMU Q&A thread.
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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this oldie but a goody: LC
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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Quote:
The shroomy 1 said:
Quote:
Peterthinks said: when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
when you work from spores on agar you isolate "sectors" or wedges of distinct growth that radiate out from a point.
It is unknown what these spore sectors will do unless you fruit them.
You could just chuck a bit of inner tissue from a mushroom into liquid culture and make a clone that way. But it is almost impossible to see contamination in a liquid culture. You better have supreme confidence in your sterile work before you knock up 20 quarts of grain with an untested LC.
Just use whatever you're cozy with. Or at least do a test grain jar.
LC to grain Wedge to wild bird seed spores to spawn bag It doesn't matter what you do or how you do it just keep it clean.
Quote:
when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
That is confusing. Please read what you just said... You contradict yourself by saying Quote:
when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
makes complete sense to me, he is saying a clone from a fruit is an isolate. I don't believe it is though, isn't it still a bunch of strains unless it was already an isolate? I would think you would have to isolate further from a MS clone, or am i wrong?
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
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Peterthinks
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Registered: 11/10/04
Posts: 2,379
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Re: staying self-sufficient [Re: RedDevil420]
#9907972 - 03/04/09 12:16 AM (15 years, 1 month ago) |
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Two spores germinate and start to grow across the dish, when they cross they combine and start a distinct section of growth called a sector.
If you cut this sector out and let it grow alone or chuck it into grain all fruits will have the traits determined by that mating of those two spores.
The reverse is also true, if you take a fruit that is the product of two spores and make a clone from it all future grows will have those traits.
a clone is like a sector like a sector is a clone.
in multi spore inoculation every fruit could be the product of two distinct spores resulting in great variation. Aggressive, slow, big, small, dark or light or any number of combinations could all show up at the same time in the same grow.
I was just stating that clones and sector isolates are more like each other than they are like multi spore mushrooms.
But hey I'm no pro, and not everything on the internet is true. I may have facts crossed or fuzzy but to the extent of what I know I think this is pretty accurate.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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The shroomy 1
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Posts: 5,543
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Re: staying self-sufficient [Re: Peterthinks]
#9909429 - 03/04/09 10:21 AM (15 years, 1 month ago) |
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I guess what confused me was that you said, "When you clone a fruit you are working with PROVEN RESULTS. Then you say that "t is unknown what these spore sectors will do unless you fruit them."
The contradiction came when you said "a clone is like one sector on a multispore dish. The clone is proven, The sector isn't.
I'm sorry. I was being a tad tooooo literal. The reason I couldn't help to pick it a part was because when I clone tissue, I often come across different sectors and will grow out at least 3 sectors before I settle on the sector that has the traits I look for in mycelium.
-------------------- AMU Q&A thread.
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ronjohn7779
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Buy a mini fridge, make LC's (clones and multispore, store the LC's in the fridge and you have a ton of syringes for a long ass. Also take lots of prints.
-------------------- "Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!
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RedDevil420
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Re: staying self-sufficient [Re: Peterthinks]
#9909906 - 03/04/09 11:49 AM (15 years, 1 month ago) |
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Quote:
Peterthinks said: when you clone a fruit you are working with proven results. a clone is like one sector on a multispore dish.
when you work from spores on agar you isolate "sectors" or wedges of distinct growth that radiate out from a point.
It is unknown what these spore sectors will do unless you fruit them.
You could just chuck a bit of inner tissue from a mushroom into liquid culture and make a clone that way. But it is almost impossible to see contamination in a liquid culture. You better have supreme confidence in your sterile work before you knock up 20 quarts of grain with an untested LC.
Just use whatever you're cozy with. Or at least do a test grain jar.
LC to grain Wedge to wild bird seed spores to spawn bag It doesn't matter what you do or how you do it just keep it clean.
I was under the impression that in MS growing, many strains come together to make a fruit...
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
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PreparationH
apply daily
Registered: 03/28/05
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Re: staying self-sufficient [Re: RedDevil420]
#9911904 - 03/04/09 05:13 PM (15 years, 1 month ago) |
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bump
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littleapplemm
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Registered: 01/14/09
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In MS there are many substrains, however, it only takes 2 spores to start a colony.
-------------------- Taco Bell tastes better with bike grease! ---------------------------------------------- See what projects I'm working on here
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