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Offlinebbf
Michael O. Jist
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Registered: 08/14/08
Posts: 62
Loc: Ten Eyes See (ahyuck)
Last seen: 14 years, 9 months
Positive and Negative Pressure Environments in Liquid Cultures
    #9885057 - 02/28/09 03:16 PM (15 years, 10 months ago)

I tweedled around with the search function for a bit, and didn't really turn anything up.  So, I have a question for you informed types.  When dealing with a liquid culture in a vacuum (as in, all holes are sealed/siliconed/whatever), what advantages, disadvantages, any differences at all would I see from maintaining a positive pressure environment in my liquid culture container?  And what about a negative pressure environment?

Operationally defined, what I am asking about is after the liquid culture container comes out of the pressure cooker and has been inoculated, what would happen from either adding sterile air or removing some of the liquid.

If the reality is that there are already a million threads with this same question, and it turns out I am just too retarded to perform an effective search, please forgive me!  I really did look.


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Correlation does not imply causation.

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Offlinebbf
Michael O. Jist
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Registered: 08/14/08
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Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: bbf]
    #9894010 - 03/01/09 11:14 PM (15 years, 10 months ago)

Should I interpret the nonanswers as "we don't know" or "it doesn't matter"?  I'm guessing the latter, I imagine there would only be negative or no significant consequences.  But I thought I'd bug you smarties since this is just wild conjecture, it's been a couple years since I've taken a bio/myco course.

Lookin' at you, RR, you're the most well informed person I can think of.
(Please be gentle if this is a stupid thing to be asking.  :frown: )


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Offlinepotatonet
Quantum Scientist
I'm a teapot


Registered: 11/13/08
Posts: 1,385
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Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: bbf]
    #9894048 - 03/01/09 11:21 PM (15 years, 10 months ago)

well one could say a lot about this

but I will go with the simple approach

very few things grow at negative pressure from 1 ATM. (high mountains have little growth of anything)

however at the bottom of the ocean... there is TONS of life!

I would go with pressurization rather than depressurization if you are insistent on experimentation.

just based on that logic (I could be wrong though!)


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Offlinebudzeno
Chillaxin
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Registered: 10/14/07
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Loc: British Columbia
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Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: potatonet]
    #9894480 - 03/02/09 01:00 AM (15 years, 10 months ago)

Well i don't know if this helps you, but Lc's will preform better with some FAE, sterile Fresh air that is "filtered". So neither pressure environments would be "Better" then a well maintained filtered Lc.
I am no expert but i do know that mushrooms, myclelium ect need air.

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Offlineghostshadow422
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Registered: 10/02/13
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Last seen: 9 years, 5 days
Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: budzeno]
    #18994586 - 10/18/13 09:03 AM (11 years, 2 months ago)

how do I release the pressure or not deal with it to begin with in my LC jars? I have been putting a cotton ball inside a empty syringe and plunging into the LC jar, is that ok or how else would it work? I think I read to keep the jars half open during pressure cooking?

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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: ghostshadow422]
    #18994600 - 10/18/13 09:10 AM (11 years, 2 months ago)

Quote:

ghostshadow422 said:
how do I release the pressure or not deal with it to begin with in my LC jars? I have been putting a cotton ball inside a empty syringe and plunging into the LC jar, is that ok or how else would it work? I think I read to keep the jars half open during pressure cooking?




Make a filtered lid. The "jars half open" is bad info.


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OfflineMidnight Cyclone
StrangerDanger
Registered: 05/29/13
Posts: 399
Loc: oo ess aye oo ess aye Flag
Last seen: 10 years, 11 months
Re: Positive and Negative Pressure Environments in Liquid Cultures [Re: Pestile]
    #18998003 - 10/18/13 11:33 PM (11 years, 2 months ago)

this is 5 years old..

ghostshadow I know you're new so when you have a question, use the search function and if you're unable to find the answer then use google. If you can't find anything at that point, consider posting a thread asking the question.

But yeah generally don't open 5 year old threads.

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