Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Mix   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinepsily
Female User Gallery

Registered: 02/11/09
Posts: 116
Last seen: 12 years, 9 months
Need to see micro pics of psilocybe mycelium
    #9849566 - 02/23/09 11:17 AM (14 years, 11 months ago)

Couple of days ago I made an LC of P.mexicana-jualisco (as my first attempt at growing - yeah, haha) and suspect that the jar is contaminated. I pipetted some to take a look under the microscope and see some mycelium - but whose?

I see very fine, less than a micron thick, translucent filaments growing from little grayish gelatinous patches (2-5mm in diameter). It looks like mold to me. Where can I find pics of psilocybe mycelium on the net for comparison? Something in 100-400x range for me, please, no SEM.

Thanks a lot in advance.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
Trusted Cultivator
OG Cultivator
Re: Need to see micro pics of psilocybe mycelium [Re: psily]
    #9849644 - 02/23/09 11:34 AM (14 years, 11 months ago)

You should see two nuclei in each cell, except those that are about to divide, where you'll see three or four nuclei.  The focus was set in this picture to see the cell nuclei, but if I had adjusted the stage up or down, I could have pulled the clamp connections into focus.  If you don't see multinucleate mycelium or clamp connections, you're growing mold.  In addition, mold hyphae is much more branched than mushroom mycelium.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Offlinepsily
Female User Gallery

Registered: 02/11/09
Posts: 116
Last seen: 12 years, 9 months
Re: Need to see micro pics of psilocybe mycelium [Re: RogerRabbit]
    #9849742 - 02/23/09 11:55 AM (14 years, 11 months ago)

Thanks RR, but what's magnification on that image? What is the width of the hyphal filament there?

Here is what I see, stained with a bit of gentian violet:


Edited by psily (02/23/09 02:27 PM)


Extras: Filter Print Post Top
Offlinescubabuddha
friend of a friend of a friend
Male


Registered: 04/08/08
Posts: 575
Loc: Boulder, CO
Last seen: 14 years, 8 months
Re: Need to see micro pics of psilocybe mycelium [Re: psily]
    #9849789 - 02/23/09 12:03 PM (14 years, 11 months ago)

cool. :thumbup:


--------------------



Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Mix   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Ive done searches, ive seen pics, i still have a ? BleaK 762 1 11/15/02 05:50 AM
by NeonBlack
* Re: Mutated 'rooms? [pic] Anonymous 1,165 5 12/28/99 12:00 PM
by Anonymous
* Please check this pic, any help? ---> Bart_Simp 1,364 5 06/10/02 09:35 PM
by We_come_in_peace
* Mycelium or Mould? mikeyASD 1,028 10 11/25/03 09:47 AM
by Protist
* Super Mushroom Emergency (Pics) Ped 1,085 12 08/07/03 05:50 PM
by DanKnugget
* pinning or just more mycelium? tuna 1,992 12 10/30/03 05:38 AM
by tuna
* Uneven mycelium growth shroom_muncher 2,144 12 10/30/03 05:41 AM
by littlejohn747
* Liquid Mycelium Culture Duration. lemunhed 1,752 7 05/24/03 02:56 PM
by lemunhed

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,829 topic views. 34 members, 195 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.009 seconds on 14 queries.