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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Wild Bird Seed

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OfflineHisStudent
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Registered: 02/01/02
Posts: 206
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When good quarts go bad
    #982865 - 10/22/02 02:57 PM (14 years, 6 months ago)

A large number of quart jars of birdseed were inoculated with various strains and in 7 days all jars were lost to bacteria.

The seed was rinsed twice in tap water, allowed to dry for about 12 hours and loaded into jars with distilled water. Water/seed ratios as described by Detheads birdseed tek were used.

Pieces of tyvek envelope was placed over the mouth of the jar and the band was screwed on (no lid was used here). The tops were wrapped in aluminum foil and the seed was allowed to soak in the distilled water inside the jars for about 24 hours. The jars were then pc'd for 1 hour at 15 psi.

When cool the jars were inoculated via multi spore syringes (3 different strains) procured from Ralph. The aluminum foil was removed and injected with 1 cc of inoculum. The hole from the needle was taped and a new piece of foil was put over the top.

The jars were incubated at 83-86F for 7 days.
Now they be stinky.............

What went wrong?
Anyone see errors in the procedure?





--------------------
I don't think I'm alone when I say I'd like to see more and more planets fall under the ruthless domination of our solar system.
- Jack Handey


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Invisiblez@z.com
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Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: When good quarts go bad [Re: HisStudent]
    #985167 - 10/23/02 12:52 AM (14 years, 6 months ago)

I have always found that an hour and a half in the pressure cooker is better for most grains. Especially birdseed. In addition to that I have always had fewer bacterial contaminations using tap water (is it the clorine that helps?). I'm not sure why that is but it seems to be the case for me.


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"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson


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OfflineMAIA
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Re: When good quarts go bad [Re: HisStudent]
    #989185 - 10/24/02 11:53 AM (14 years, 6 months ago)

Next time don't innoculate one of the jars and use it as a test jar, that way you can narrow down if the problem is before innoculation or after.
It could be the sterilization process, PC the jars for more time. Or the innoculation process, did you flame the needle between jars ? Did you innoculate by openning the jar ?
If you took all precautions, it could be the syringes.

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlineuser_exists
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Registered: 10/05/02
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Re: When good quarts go bad [Re: MAIA]
    #990963 - 10/24/02 10:45 PM (14 years, 6 months ago)

how dare you point fingers at ralphs syringes maybe it was you!


What type of birdseed did you use?



Edited by user_exists (10/25/02 12:58 AM)


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OfflineMAIA
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Re: When good quarts go bad [Re: user_exists]
    #992548 - 10/25/02 02:19 PM (14 years, 6 months ago)

I just pointed out every possibility i could think of. I call it being ojective and it's handy when you need to narrow down the problem.
Anyway, i know ralph from this boards for a long time, have received prints from him, he's a nice guy and i respect his work, i know he has a very good reputation in terms of quality of his produtcs, stated by me. Please we don't need that kind of remarks over here, be objective.
BTW, the only finger i point is the middle one at you :tongue:.

MAIA
:grin:


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineSeussA
Error: divide byzero

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Re: When good quarts go bad [Re: user_exists]
    #992731 - 10/25/02 03:44 PM (14 years, 6 months ago)

Even the best of us get contamination from time to time... Last time I checked, Ralph was human, and people can and do make mistakes. I am not flaming Ralph here, I have never had any problems with his products... but to imply that his work is immaculate would be improper. Anybody that claims to have never produced a bad syringe or print is either a lier, hasn't made very many, or is working in a class 10 clean room.


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OfflineHisStudent
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Re: When good quarts go bad [Re: HisStudent]
    #993213 - 10/25/02 07:57 PM (14 years, 6 months ago)

Settle down their User exists.

First...
I was not insinuating that the syringes were bad!!!!

When I said that the syringes were from Ralphster, my intent was to show that they were professionally prepared syringes. I said this so anyone reading this post could rule out the contaminated syringe issue and focus their attention on the procedure. I believe that the contamination was my error.

Second...

I would not come on a public forum to defame Ralphster.
If I had a problem with one of his products I would contact him via PM or e-mail
Not make accusations here or any other board.

How dare YOU assume that I meant anything other than what I said.

Now that that's out of the way.
Thanks z@z and MAIA for the replies. I am rethinking and revamping sterile procedure and looking to adjust the water content for a longer PC time without bursting to many grains. The un-inoculated control jar sounds like a good idea as well....


--------------------
I don't think I'm alone when I say I'd like to see more and more planets fall under the ruthless domination of our solar system.
- Jack Handey


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Offlineuser_exists
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Registered: 10/05/02
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Re: When good quarts go bad [Re: HisStudent]
    #993908 - 10/26/02 01:24 AM (14 years, 6 months ago)

wow sorry I thought it was very obviose I was joking notice the smily face as a post icon. I know that if anyone was going to blame raplhs spores they would come right out and say it I just got tired of reading posts and felt like typing up a post. I know little about ralph but seems really nice giving away prints and all so I just wanted to add a little humor but maybe now on I'll just stick to my own quistions and posts.


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OfflineMAIA
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Re: When good quarts go bad [Re: user_exists]
    #994559 - 10/26/02 12:48 PM (14 years, 6 months ago)

Hey, i also though you might be kidding but your post icon certainly was/is not a smile, you can blame that.
Smile= :smile: not  :tongue:.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineZodiacz
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Registered: 10/02/02
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Re: When good quarts go bad [Re: HisStudent]
    #994654 - 10/26/02 01:51 PM (14 years, 6 months ago)

I would suspect that the seed had too much water.  In my experience with the seed and grain process I seem to have had contamination whenever the water level was a bit too high. Soaking overnight then PC'ing should be enough without adding much additional water before cooking. You never want to see water dripping around the grains after PC. Hope this helps next time. :smile:


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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Wild Bird Seed

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