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Offlineanakin3197
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FIRST FAILURE WITH AGAR
    #9823146 - 02/18/09 10:26 PM (15 years, 1 month ago)

So my first try using agar didnt work out to well. i followed the grocery store tek to the t. they both smell nasty as hell, and one has some shit growing on it. smell like food that has been set out, and started to grow mold on it. the strains i used were pr and p menance. the pics are of the pr. theres no growth on the p menance, jus smells bad.




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Offlinecalifarmer619
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823164 - 02/18/09 10:29 PM (15 years, 1 month ago)

ooohhh SNAP :coleman:


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InvisibleJitsu
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Re: FIRST FAILURE WITH AGAR [Re: califarmer619]
    #9823212 - 02/18/09 10:36 PM (15 years, 1 month ago)

If you are not using a flowhood make sure you use a glovebox.


I sterilize my agar for 35 mins @ 15PSI then I let it cool for a few hours.


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OfflineDamion5050
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823218 - 02/18/09 10:36 PM (15 years, 1 month ago)

Quote:

anakin3197 said:
So my first try using agar didnt work out to well. i followed the grocery store tek to the t. they both smell nasty as hell, and one has some shit growing on it. smell like food that has been set out, and started to grow mold on it. the strains i used were pr and p menance. the pics are of the pr. theres no growth on the p menance, jus smells bad.








Sorry to hear, if you could pick out the healthy myco you can transfer it away from contamination.. did you use a glovebox I hope..

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Offlinehoudinihar
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823235 - 02/18/09 10:38 PM (15 years, 1 month ago)

it is definitely best to PC these bad boys. I usually put about 500 ml fluid in my quart glass canning jar, add my agar and any additives, put the lid on, then foil and sterilize for 45-60 minutes at 15 PSI. i use the non-vented (ie, no nail hole lids) and they suck a vacuum as they cool. i have one right now that has been sitting around for a few weeks solid and perfect, while the other was poured after cooling and before gelling. no peroxide in these guys this time.

here's some pics with various species:





here's one of the same with contamination--out of the 20 plates, i had 2 that got contaminated--has to be my technique. i actually took mycelia from the contaminated speicmen and plated out again away from the contamination. i like this strain and wanted to save it.



houdinihar

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Offlineanakin3197
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Re: FIRST FAILURE WITH AGAR [Re: Damion5050]
    #9823248 - 02/18/09 10:39 PM (15 years, 1 month ago)

hell there isnt any myco. i didnt use a glovebox, but i used a small still aired room, and sterlized the fuck out of it

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OfflineDamion5050
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823263 - 02/18/09 10:41 PM (15 years, 1 month ago)

Quote:

anakin3197 said:
hell there isnt any myco. i didnt use a glovebox, but i used a small still aired room, and sterlized the fuck out of it





:failboat:


You have to have at least a glovebox, I wasted 20 dishes with my first agar attempt, I though I was doing everything right, I tried doing it in a closet, sterlized the hell out of it and still failed horrible..  Minimum need is a glovebox.

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Offlineanakin3197
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823274 - 02/18/09 10:42 PM (15 years, 1 month ago)

i pcd them for 35 minutes. im guessing the red in the pic is some kind of bacteria, and i know the other is mold

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Offlineanakin3197
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823286 - 02/18/09 10:44 PM (15 years, 1 month ago)

so would you say that the other ones that i have put anything in are bad as well? there in the fridge, right now, in a ziploc bag, i sterlized the inside with alochol before i put them in it

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Offlinecalifarmer619
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Re: FIRST FAILURE WITH AGAR [Re: anakin3197]
    #9823459 - 02/18/09 11:10 PM (15 years, 1 month ago)

I think you wait too long to transfer.


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InvisibleShroominit
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Re: FIRST FAILURE WITH AGAR [Re: califarmer619]
    #9823507 - 02/18/09 11:15 PM (15 years, 1 month ago)

No GB is almost a sure way to fail with petris.

You can use little containers like:
http://www.shroomery.org/forums/showflat.php/Number/9705498#9705498

With no GB or anything. They never hit open air, but petris will not work. Also, get rid of that ASAP. Bad for your grow area, bad for your health.

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Offlineanakin3197
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Re: FIRST FAILURE WITH AGAR [Re: Shroominit]
    #9825780 - 02/19/09 11:02 AM (15 years, 1 month ago)

i might try to get some of those and give them a try. and maybe ill try to make a gb. but for some reason i think the mixture went bad, that i didnt do something right

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