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Invisiblegoldieman
On some other shit!


Registered: 08/15/07
Posts: 296
Loc: midwest
FAILED ATTEMPT AT BABBY BOTTLE TEK: NEED HELP!
    #9819925 - 02/18/09 01:04 PM (15 years, 1 month ago)

i recently tried agars babby bottle tek, as i was starting from scratch with new strains and wanted a quick way to get the spores germinated. i used 4 ml of light karo to 100ml of spring water. first try: pc'ed for 20 minutes once pressure reached 15psi. removed from heat and let cool down. when i removed jars from pc, they were very yellow in appearance. nothing floated in bottles, but still yellow.
second attempt: dumped bottles. used same measurements as before. only this time i let pc reach 10 psi. once there, it fluctuated between 10 and 13 psi for 15 minutes. after 15 minutes removed from heat and let cool. when i opened pc, bottles were still yellowish. so i went ahead and knocked up with 3 cc's of spore solution anyway since i read a few posts that said it would be ok. i have had in a dark cabinet for 4 days now. temps are mid 80's. i can see strandy clumps of what look like spores. the syringes i used i got from sporeworks and after shaking, the spores stayed together in the syringe like as if they had germinated already! all four syringes, different strains. i dont know whats going on here, but did i damage the lc before knocking up? was it actually sterilized? it didnt reach 15psi and was only between 10-13 psi for 15 min. any comments or suggestions appreciated

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OfflinePreparationH
apply daily


Registered: 03/28/05 Happy 19th Shroomiversary!
Posts: 18,345
Loc: Amsterdam
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Re: FAILED ATTEMPT AT BABBY BOTTLE TEK: NEED HELP! [Re: goldieman]
    #9820081 - 02/18/09 01:47 PM (15 years, 1 month ago)

...you just said in the bottle you see growth and its only been 4 days.  My guess is its growing lol and 4 days doesnt seem like much time id say wait a few more days.

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