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Offlinezeronio
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Registered: 10/16/01
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Loc: Slovenia
Last seen: 5 years, 19 days
Wild clones
    #981910 - 10/22/02 06:01 AM (18 years, 11 months ago)

I cloned Kuehneromyces mutabilis, which is a good edible but mostly igored because of it resemblance to deadly Galerina marginata.
http://www.grzyby.pl/gatunki/Kuehneromyces_mutabilis.htm
http://users.skynet.be/jjw.myco.mons/Galerina_marginata_1.html

Has anybody grown this species?
Here are some pics of mycellium:






What do you think about this?
Yes, that's Kuehneromyces mutabilis!
That's pure contamn
You made a false ID and you'll die eating it!
I don't know





Votes accepted from (12/31/69 07:00 PM) to (No end specified)
View the results of this poll



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OfflineSuntzu
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Registered: 10/15/99
Posts: 1,396
Last seen: 5 months, 29 days
Re: Wild clones [Re: zeronio]
    #982269 - 10/22/02 10:22 AM (18 years, 11 months ago)

It looks like a clean clone of whatever the original species was; Nice work!



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Offlineaural
Hola Amigos!
Registered: 05/04/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 17 years, 5 months
Re: Wild clones [Re: zeronio]
    #982270 - 10/22/02 10:22 AM (18 years, 11 months ago)

Were you able to find a taxonomic key for it?
Sounds like you're not quite convinced...


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Offlinezeronio
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Registered: 10/16/01
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Re: Wild clones [Re: aural]
    #982395 - 10/22/02 11:22 AM (18 years, 11 months ago)

I'm 99% sure about ID. I got confirmation of an expert but he told me that he once made a mistake and got poisoned with galerina - that's why it's not 100%.
Galerina marginata contains alpha-amanitin (like destroying angel) and I did a Wieland test that would show large amounts of this toxin - I will repeat it I ever grow it.


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Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Wild clones [Re: zeronio]
    #982526 - 10/22/02 12:31 PM (18 years, 11 months ago)

When are you sending me a spore print? :wink:

-pSyCo 


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Offlinezeronio
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Registered: 10/16/01
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Re: Wild clones [Re: psyconaut]
    #988427 - 10/24/02 02:36 AM (18 years, 10 months ago)

I also tried cloned rare Pleurotus dryinus. However I'm not sure if I got a clean culture or some sort of contamination.
Here is a quote from
http://fp.bio.utk.edu/mycology/Pleurotus/pleurotusisg.htm#ISG%20IX.
"In culture, P. dryinus is unique in forming a turf of simple arthrosporophores and dry, easily disarticulated chains of tan to brown arthrospores. This character easily separates cultures of P. dryinus from cultures of P. levis, which are without anamorphic spores."

Here are my petris:









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Offlinezeronio
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Registered: 10/16/01
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Re: Wild clones [Re: zeronio]
    #988453 - 10/24/02 02:46 AM (18 years, 10 months ago)

I wish more people would post pictures of their cultures. It would be easier to find out if cloning suceeded.

This is supposed to be Coprinus comatus:



and Macrolepiota procera:


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Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Wild clones [Re: zeronio]
    #988509 - 10/24/02 03:15 AM (18 years, 10 months ago)

Your petris look consistent with the description. Wish I could be of more help :/

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Wild clones [Re: zeronio]
    #988513 - 10/24/02 03:18 AM (18 years, 10 months ago)

You're doing some nice work :smile:

Yes, it'd be great if more folk posted their work and pics. It's a shame more folk here aren't as interested in mushrooms as they are in getting high :wink:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Offlinezeronio
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Registered: 10/16/01
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Pleurotus dryinus [Re: psyconaut]
    #991911 - 10/25/02 05:24 AM (18 years, 10 months ago)

I decided to ask two known Pleurotus experts about this culture and surprisingly they both answered me right away. (I thought they will consider my mails as junk :grin:)
Here are their interesting answers:

Dr. Ronald H. Petersen

    You cultures do not look like those of P. dryinus. First; cultures of
P. dryinus should be dry, yours looks wet. Second; cultures of P. dryinus
do not usually form mycelial ropes or plates, but your fig. 3 shows such
mycelial plates. Third; cultures of P. dryinus form brown conidia. Fourth;
From cloned cultures from fruitbodies, there should be common clamp
connections on all hyphae. Finally; all Pleurotus cultures form microscopic
liquid droplets on short side-branches of hyphae. If there are no liquid
droplets, the culture is not from Pleurotus.
    If you examine your culture microscopically, and if you still have
doubts, send the culture to me and I will look at it myself.
    I think you will get the same opinion from Nadya Psurtseva in St.
Petersburg.


Nadya Psurtseva

Yes, P. dryinus culture looks differently from the other Pleurotus. It grows
much slower and has yellowish or dark brown pigment on the colony mat. What
about micromorphology? Check clamps. If they are present, it is a good chance you have got a really P. dryinus culture.

 


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InvisibleZen Peddler
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Re: Wild clones [Re: zeronio]
    #993274 - 10/25/02 08:22 PM (18 years, 10 months ago)

It looks like mycelia, but it could be a myriad of different species


--------------------


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InvisibleCordyceps
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Registered: 09/15/09
Posts: 337
Re: Wild clones [Re: zeronio]
    #11741647 - 12/30/09 08:09 AM (11 years, 8 months ago)

Hi Zeronio,

I have also tryed to clone wild P. dryines but it's very hard to get a good mycelium from the 4 petri dishes I cloned 1 has survived. Al the other where contaminated  :sad:


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InvisibleJef
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Registered: 12/03/08
Posts: 764
Loc: near Duncan, BC
Re: Wild clones [Re: zeronio]
    #11746859 - 12/31/09 04:25 AM (11 years, 8 months ago)

Your poll tells me I didn't vote for anything. I vote for I hope you're not 1% unlucky.

And I hope you're more organized than I am...

I might mix them up or rub the description off the spawn jar, and eat them as black poplar, or pluteus cervinus.

Quote:

zeronio said:
I'm 99% sure about ID. I got confirmation of an expert but he told me that he once made a mistake and got poisoned with galerina - that's why it's not 100%.
Galerina marginata contains alpha-amanitin (like destroying angel) and I did a Wieland test that would show large amounts of this toxin - I will repeat it I ever grow it.





Feed the first crop to your mother in law.

It's an honest mistake!:eek:

Quote:

psyconaut said:
When are you sending me a spore print? :wink:

-pSyCo 




Let him try it first!

Be patient....it ca take seven days after you eat it.  I hear that you feel a lot better after the first two days...:smile:

Did your expert say how much he ate ?:wow:

I agree your "dryinus" cultur looks like mycelium, not contam.

Also the procera looks very similar to Chlorophyllum rhacodes mycelium, a mushroom which is very similar.  It is slow to grow on agar.  How about procera ?

It IS nice work you're doing.  I hope you live.  Eat LESS mutabilis than your mother in law does!

Good growing,

Jef




Edited by Jef (12/31/09 04:47 AM)


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