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OfflineMyce
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Registered: 01/29/09
Posts: 79
Last seen: 15 years, 7 months
My 1st piss poor attempt at making agar + ?'s
    #9809374 - 02/16/09 05:08 PM (15 years, 10 months ago)

So today I decided to make my first stab at concocting agar but I didn't do the best job. I'll run through what I did and my mistakes and it would be great if someone would let me know if I should just start again.

First off I got the antibiotic malt extract agar from fungi perfect. I mixed 3 and 1/2 tablespoons into a Crown Royal (1/5) bottle along with a little over 2 cups of distilled water. This is were my problems began. I realized I didn't have a funnel so the only thing I could think to use to get the agar powder in the bottle was a folded piece of computer paper. Not sure if this will cause contam's but I thought I'd be fine because I was still about to pressure cook it. Next I mixed in the water and shook it up so the powder dissolved and I put a piece of tyvek under the lid. The bottle fell over right before I PC'ed it getting the tyvek wet, not sure if thats a problem. Next I began my PC'ing and as steam began to come out I realized I should have put some extra water in to help reduce boil over. I let the PC cool for a second and then added some water and started the process over again. The PC began to release steam again and I realized there was too much water this time because it was shooting out every possible place. I let the PC cool once more and opened it to find the bottle submerged in water. I took the bottle out, replaced the tyvek, shook it up (the bottom of the agar was jelly like),and added the correct amount of water. I am about 10 min into pressure cooking it and just wanted to know if all these problems will affect my agar. Also should I keep the cap of the crown loose while I PC? Thanks in advance and sorry it was so long. I'll update later tonight when I begin to pour into pitri's because I am sure I'll end up messing something else up.

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InvisibleFooMan
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Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9809389 - 02/16/09 05:11 PM (15 years, 10 months ago)

You messed up when you purchased antibiotic agar. You should only be using that stuff to clean up already contaminated cultures.

That was one hell of a funny story :lol:


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OfflineMyce
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Registered: 01/29/09
Posts: 79
Last seen: 15 years, 7 months
Re: My 1st piss poor attempt at making agar + ?'s [Re: FooMan]
    #9809421 - 02/16/09 05:17 PM (15 years, 10 months ago)

Haha so the antibiotic malt extract agar wont work like regular malt extract agar? If so that sucks and I messed up even more than I thought (and I thought I messed up alot)

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OfflineStuckintheskY
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Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9809434 - 02/16/09 05:20 PM (15 years, 10 months ago)

No worries bro, just start over, at least you learned something.


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"All that we are is the result of what we have thought. The mind is everything. What we think we become. "

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Invisiblenoobieshroomie
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Re: My 1st piss poor attempt at making agar + ?'s [Re: StuckintheskY]
    #9809509 - 02/16/09 05:32 PM (15 years, 10 months ago)

use this thats what i do
its cheap,easy and it works well
http://www.shroomery.org/forums/showflat.php/Number/7081785#7081785
ill post pics in a sec to prove it


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineMyce
The Dude Abides

Registered: 01/29/09
Posts: 79
Last seen: 15 years, 7 months
Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9809516 - 02/16/09 05:33 PM (15 years, 10 months ago)

Well is there anything I can do with this antibiotic agar or should I just toss it after I get it out of the PC? Kinda sucks cuz I spent like $40 on this shit

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Invisiblenoobieshroomie
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Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9809537 - 02/16/09 05:36 PM (15 years, 10 months ago)

use this thats what i do
its cheap,easy and it works well
http://www.shroomery.org/forums/showflat.php/Number/7081785#7081785
ill post pics in a sec to prove it

    -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineRogerRabbitM
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Re: My 1st piss poor attempt at making agar + ?'s [Re: noobieshroomie]
    #9809808 - 02/16/09 06:27 PM (15 years, 10 months ago)

It should be OK.  I usually fill the PC about half way full of water when I sterilize agar, because it helps prevent boilover as the PC cools.  The antibiotic agar will be fine.  I always keep a can of it around for cloning, but use regular agar for most uses, because I actually want to see if I have bacteria present, so I can take extra care to get it cleaned up.  You can use a bit less of the powder next time.  I use about 3 1/2 tablespoons of FP agar for three cups of water, which is slightly less than called for, but does fine.

Pour your dishes when the bottle has cooled off enough to handle with latex gloves and not burn yourself.  If you wait too long, it gets thick and can't be poured easily, and if you pour too soon, it causes excess condensation in the dishes.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineMyce
The Dude Abides

Registered: 01/29/09
Posts: 79
Last seen: 15 years, 7 months
Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9810822 - 02/16/09 09:39 PM (15 years, 10 months ago)

Alright well I went through with the process and I finished pretty much like I started. Depending on the results I might have to postpone an agar habit until I get enough money for a flowhood. I am way too big and clumsy for my Rubbermade 64 quart glovebox. Also I took a break from the disaster and came back to find my agar had set. Can I heat this back up in boiling water? I had 7 dried trays and tried to inoculate 5 with a ms. 3 of them got covered in spores and liquid but I was able to get 2 of them to be somewhat decent. I'll continue to update on my longshot operation so new people know how to better avoid some of the mistakes I'm having.

Lastly, any tips or methods to using Gladware clingwrap? I was really struggling with that shit. Wish me luck

Edit: I'm going to try out the grocery store agar tek if I fail at this one.

Edited by Myce (02/16/09 09:42 PM)

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OfflineMyce
The Dude Abides

Registered: 01/29/09
Posts: 79
Last seen: 15 years, 7 months
Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #9813809 - 02/17/09 11:40 AM (15 years, 10 months ago)

So I reheated the rest of my agar and  plan on pouring soon but I am about to go get 1/4 pint jars and fill them with newly made agar and PC, so I don't have to deal with pitri's anymore (I'm way to clumsy in a glove box). Should I inoculate while the agar is still liquid-like but cool, or wait till it sets? Also for my 1/4 pint jars should I add a hole to the lid and cover it with silicone for injections or just open the lid of the jar a bit and inject through there? Thank ya'll so much for all the help

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Offlinekomsija
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Registered: 03/31/09
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Re: My 1st piss poor attempt at making agar + ?'s [Re: Myce]
    #10098985 - 04/03/09 05:47 AM (15 years, 9 months ago)

this is my plates after 19 days....i have feeling just like they dont have enougf power to cover the plate with micelium(oyster):(


i use PDA home made agar

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