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Amazon Shop for: ½ Pint Jars, Agar

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Offlinec0ma
the pimp

Registered: 09/22/02
Posts: 1,249
Last seen: 11 years, 1 month
Man...this shit sux :(
    #970230 - 10/17/02 08:12 PM (14 years, 4 months ago)

Ok i tried my best at being sterile and stuff, but i have so many doubts in myself that i lost hope in all my jars, i just inculated them but everything didnt seem right, like the substrate was really loose...the nidle turned copleatly blacked after i tried to sterileze with a lighter, and then the worst thing happend.. the stupid flame fromt the lighter burnt plastic on it and the black smoke started coming out...i used alota lysol but still very unhappy with my work...
im gonna check on my jars every 5 days, if nothing happens in 2 weeks then i'll just give up with it untll better times...

i was wondering if any1 had ANY result when did something not copleatly hot they wanted to do it... maybe didnt sterilize the jars enough in a first place or didnt sterilize the niddle atall...

this really bothers me and now i'm all depressed couse i had so many plans :frown:


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Offlinehappygrins
addict

Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 12 years, 23 days
Re: Man...this shit sux :( [Re: c0ma]
    #970252 - 10/17/02 08:16 PM (14 years, 4 months ago)

Hmm.. well I hope everything works out for you.  Next time, plan ahead so you have time to think your steps through.  Make sure you have no interruptions and stay focused.  Hopefully you'll get lucky and somthing will happen for ya tho.  Just learn from your mistakes.

--happygrins :grin:


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Offlinedrenaline
shroomitist

Registered: 10/06/02
Posts: 81
Loc: A Moon of Jupiter
Last seen: 12 years, 7 months
Re: Man...this shit sux :( [Re: c0ma]
    #970260 - 10/17/02 08:20 PM (14 years, 4 months ago)

As long as you didnt physically touch the needle or allow it to touch anything, I would think you should be fine  :smile:


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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
Re: Man...this shit sux :( [Re: c0ma]
    #970262 - 10/17/02 08:20 PM (14 years, 4 months ago)

I also accidentally burned the plastic mount for the needle on my syringe.. but i put it out and continued working and the results were fine.


--------------------
Be Here Now


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 8 months
Re: Man...this shit sux :( [Re: c0ma]
    #970274 - 10/17/02 08:24 PM (14 years, 4 months ago)

give it more than two weeks, i wasn't sterile at all. no gloves, lysol, nothing. just cleaned with a little bleach. and i forgot to sterilize my lighter after the first jar. and i did 10 jars. after 15 days there is growth on all of them. just be patient. agar is a lot quicker but you have to be really sterile. i was spraying lysol on myself. i smell like an old folks home, but everything is very sterile. laterzzzzz

de-=-


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OfflineNoVestal
The Patient

Registered: 10/13/02
Posts: 50
Loc: Damning my soul with a ti...
Last seen: 14 years, 3 months
Re: Man...this shit sux :( [Re: MsPacMan]
    #973756 - 10/19/02 02:33 AM (14 years, 4 months ago)

I know this is so much wasted finger movement but don't take psychadelics if you don't like yourself or don't feel good about yourself. In other words, now is not the time to be doing this. Wait. Grow. Find your Self. Then try again.


--------------------
______________________________________

http://www.angelfire.com/darkside/lightside9/


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Invisibleangryshroom
Stranger
 User Gallery

Registered: 12/18/01
Posts: 7,262
Re: Man...this shit sux :( [Re: c0ma]
    #973845 - 10/19/02 03:11 AM (14 years, 4 months ago)

If you're doing the PF tek, you shouldnt have any problems. ... its pretty hard to contaminate jars :smile:


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Offlineeternal
Greasy Granny'sGopher GravyTaste Tester

Registered: 08/27/02
Posts: 251
Loc: Here? Is there any where ...
Last seen: 10 years, 6 months
Re: Man...this shit sux :( [Re: angryshroom]
    #974357 - 10/19/02 10:18 AM (14 years, 4 months ago)

Hello,

I have used the PF Tek many times with great success.  I have never used a flame to sterilize the needle.  I wipe the needle down with 91% alcohol everytime I innuculate a new jar.  Never had contam issue with it.  My suggestion is stay away from open flame period.  If you fail this time try again.  That is the beauty of this hobby.  Eventually you will hit pay dirt.

Peace out! :grin: 


--------------------
No matter where you go. There you are!



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InvisibleCptnMaxMushroom
enviromentaladvocate
Registered: 08/26/02
Posts: 138
Re: Man...this shit sux :( [Re: eternal]
    #974415 - 10/19/02 11:01 AM (14 years, 4 months ago)

Flame sterilization and alcohol wipes are the biggest forms of overkill I know of.

Your sticking your needle into what is supposed to be a sterile jar of substrate so why would one think furhter sterilization is necessary? It's not going to get contaminated by sticking it into sterile substrate. If for any reason you had doubt as to wether it was sterile, don't stick it in that jar!

I can only imagine the amount of time one leaves the needle exposed as they flame and wipe after each innoculation....4 times on each jar, 10 or so jars, thats 40 wipes....prolly takes an hour to innoculate a few jars...what a waste of time and effort! lol!


--------------------
Visit the SporeLab....
http://www.sporelab.com
A trip you'll never forget!


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Man...this shit sux :( [Re: c0ma]
    #974416 - 10/19/02 11:03 AM (14 years, 4 months ago)

My first grow should have been a disaster. I boiled too long, didn't sterilize the needle, forgot to shake the syringe and actually dropped and kicked two cakes across the floor while trying to birth them. Amazingly, they fruited pretty well.  :shocked:

You'll be fine. Learn from your mistakes.  :laugh: 


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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InvisibleCheeba
the shroomyqueen

Registered: 09/23/02
Posts: 158
Loc: atop my secret mountain
Re: Man...this shit sux :( [Re: c0ma]
    #974433 - 10/19/02 11:11 AM (14 years, 4 months ago)

Live and learn, my friend!  :smile:
Now, next time you'll know what you DON'T want to do!!!  Don't get discouraged and give up.  This is a hobby that you'll aquire expertise with experience!!!  Atleast if you get some shroomies from this batch...you'll see what resilient little bastards they are!
Love, Cheeba 


--------------------
Carpe Diem <*))))><
Theres one sweet whirld out there!!
Now remember, you are unique! Just like everybody else!


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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