Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinejohnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 6 months
Are my disposable petris sterile?
    #9693519 - 01/28/09 09:40 AM (15 years, 2 months ago)

The guys in the lab supplies place assured me they were, but when I got them home I gave the sleeve a little squeeze and found it to not be air tight. They're packed in what appears to be a bog-standard clear plastic sleeve. It doesn't look anything like any kind of breathable microfibre or anything clever like that. Since the sleeve was leaking air is it likely that these petris are not good? The fuckers.


--------------------
[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

Extras: Filter Print Post Top
OfflineABC
Stranger
 User Gallery


Registered: 11/16/08
Posts: 1,439
Last seen: 1 year, 11 months
Re: Are my disposable petris sterile? [Re: johnny_poke]
    #9693544 - 01/28/09 09:52 AM (15 years, 2 months ago)

I believe they're supposed to be usable right out of the sleeve, but i'm not sure if the sleeve was leaking...

Extras: Filter Print Post Top
Offlinemushroomhunter10
Jack-Of-All-Trades
Male User Gallery


Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 8 years, 9 months
Re: Are my disposable petris sterile? [Re: ABC]
    #9693551 - 01/28/09 09:54 AM (15 years, 2 months ago)

Just wipe the insides of them with alcohol.

Problem solved! :smile:


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: mushroomhunter10]
    #9693594 - 01/28/09 10:08 AM (15 years, 2 months ago)

Quote:

mushroomhunter10 said:
Just wipe the insides of them with alcohol.

Problem solved! :smile:



Horrible advice. that is a sure fire way to introduce more contams.

As long as the bag isn't torn wide open then they should be fine. I believe the bag should have been air tight but a small leak shouldn't be a problem unless someone was squeezing it and then stretching it like an accordion.

I never use a whole sleeve in one sitting so I open the bag, pull out 5-8 dishes and just fold the bag close. The rest do not contaminate.

If you went ahead and wiped the inside with alcohol like the jackass suggested then and went ahead and used them and they still contaminated what would you do then? You wouldn't know if you caused it with the alcohol wipe or if they come dirty.
Also, if wiping them with alcohol worked then the plastic ones would be reusable and you would never have to buy new ones.
Point is, that was some stupid shit to say and do not attempt to wipe them with alcohol.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Offlinemushroomhunter10
Jack-Of-All-Trades
Male User Gallery


Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 8 years, 9 months
Re: Are my disposable petris sterile? [Re: tahoe]
    #9693650 - 01/28/09 10:37 AM (15 years, 2 months ago)

I KNOW for a FACT that it works.

You just have to wipe them inside of a glove box or something.

I'm not a jackass either so please... I've used alcohol to wipe down many many plastic petris with zero contams. Must be in a glove box though.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 10 months
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: mushroomhunter10]
    #9693669 - 01/28/09 10:44 AM (15 years, 2 months ago)

Quote:

mushroomhunter10 said:
Just wipe the insides of them with alcohol.

Problem solved! :smile:




You have just been Tahoed :rofl:

But you really should not have given that advice, it is the biggest piece of rubbish out.

Alcohol does not sterilize, only sanitize.

As said above, you should be fine, especially if the dishes haven't opened inside the sleeve.


--------------------
Newcomers guide-----> For all things shroomy

Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 10 months
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: mushroomhunter10]
    #9693681 - 01/28/09 10:50 AM (15 years, 2 months ago)

Quote:

mushroomhunter10 said:
I KNOW for a FACT that it works.

You just have to wipe them inside of a glove box or something.

I'm not a jackass either so please... I've used alcohol to wipe down many many plastic petris with zero contams. Must be in a glove box though.




Well, you are wrong, I'm sorry to say, alcohol, especially ust wiping with alcohol will not sterilize a single thing.

Why would we PC or sterilize them by other means if that were so.

I dare you to put some trich spores or rub an anal swab on a petri dish then wipe it with alcohol and try and grow something in it without contaminating.


--------------------
Newcomers guide-----> For all things shroomy

Extras: Filter Print Post Top
InvisibleGerman Kahuna
Facepalmer of Stoopid
Male User Gallery


Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: tahoe]
    #9693798 - 01/28/09 12:06 PM (15 years, 2 months ago)

Quote:

tahoe said:
I never use a whole sleeve in one sitting so I open the bag, pull out 5-8 dishes and just fold the bag close. The rest do not contaminate.




Exactly what I do. I have 20 dish sleeves and most of the time prepare 10. After I fold the sleeve up, I wrap it in aluminum foil fresh from the roll. No contam problems.


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

Extras: Filter Print Post Top
Offlinejohnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 6 months
Re: Are my disposable petris sterile? [Re: Nibin]
    #9693869 - 01/28/09 12:32 PM (15 years, 2 months ago)

Cool. Thanks alot you guys :smile:


--------------------
[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: johnny_poke]
    #9693909 - 01/28/09 12:40 PM (15 years, 2 months ago)

Quote:

johnny_poke said:
Cool. Thanks alot you guys :smile:



Who are you thanking and what knowledge are you taking away from this?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
InvisibleJitsu
JKD Love
Male User Gallery


Registered: 11/12/08
Posts: 1,073
Re: Are my disposable petris sterile? [Re: johnny_poke]
    #9693927 - 01/28/09 12:43 PM (15 years, 2 months ago)

just in case you were wondering... dont try to steam sterilize those petris or PC them... it will not end up to well...

IME :<


--------------------
Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

How I get my Pinsets
The Capabilities Of A Shotgun FC

Extras: Filter Print Post Top
OfflineMentonaut
Stranger

Registered: 09/09/08
Posts: 168
Last seen: 13 years, 7 months
Re: Are my disposable petris sterile? [Re: tahoe]
    #9693958 - 01/28/09 12:46 PM (15 years, 2 months ago)

Quote:

tahoe said:
Quote:

johnny_poke said:
Cool. Thanks alot you guys :smile:



Who are you thanking and what knowledge are you taking away from this?




So now there are quizzes?

Extras: Filter Print Post Top
Offlinejohnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 6 months
Re: Are my disposable petris sterile? [Re: Mentonaut]
    #9694023 - 01/28/09 12:55 PM (15 years, 2 months ago)

Relax Tahoe. I am thanking Kahuna, Nibin and yourself for advising me that the dishes are more then likely OK. The sleeves don't appear to be particularly dishevelled, and the dishes are stacked politely and not open. The place was very above board so let's just hope the air leakage is nothing. It just eems odd that the sleeves would be so weakly sealed since sterility of these items is obviously paramount.


--------------------
[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

Extras: Filter Print Post Top
InvisibleJitsu
JKD Love
Male User Gallery


Registered: 11/12/08
Posts: 1,073
Re: Are my disposable petris sterile? [Re: johnny_poke]
    #9694049 - 01/28/09 01:00 PM (15 years, 2 months ago)

Aren't most petri's sterilized with the lamps?(I forget the name) If they haven't been opened in the bag I believe you are still good to go!

Please someone correct me if I am wrong.


--------------------
Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

How I get my Pinsets
The Capabilities Of A Shotgun FC

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: Jitsu]
    #9694390 - 01/28/09 02:16 PM (15 years, 2 months ago)

gamma radiation


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Offlinemushroomhunter10
Jack-Of-All-Trades
Male User Gallery


Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 8 years, 9 months
Re: Are my disposable petris sterile? [Re: tahoe]
    #9694957 - 01/28/09 03:43 PM (15 years, 2 months ago)

Then why is it that we sterilize our gloves, the insides of the glove boxes, and tools with alcohol?

It's how I've reused many of them already...

What other methods are there for the disposables? I suppose boiling is good enough too. Not sure if it can withstand the temps.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 10 months
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: mushroomhunter10]
    #9694987 - 01/28/09 03:49 PM (15 years, 2 months ago)

Quote:

mushroomhunter10 said:
Then why is it that we sterilize our gloves, the insides of the glove boxes, and tools with alcohol?

It's how I've reused many of them already...

What other methods are there for the disposables? I suppose boiling is good enough too. Not sure if it can withstand the temps.




No you don't.

You sanitize your gloves and glovebox with alcohol and you sterilize your instruments with a FLAME.

Alcohol does NOT sterilize it only sanitizes (i.e. kills off part of the contaminants).

And don't tell me I'm talking crap, I did get halfway through medical school before realizing what a shitty job it would entail so I know what I am talking about.


--------------------
Newcomers guide-----> For all things shroomy

Extras: Filter Print Post Top
Offlinejohnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 6 months
Re: Are my disposable petris sterile? [Re: Nibin]
    #9695105 - 01/28/09 04:06 PM (15 years, 2 months ago)

I really wanted to get glass ones but the difference in price is enormous. Maybe next time.


--------------------
[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

Extras: Filter Print Post Top
InvisibleGerman Kahuna
Facepalmer of Stoopid
Male User Gallery


Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
Trusted Cultivator
Re: Are my disposable petris sterile? [Re: johnny_poke]
    #9695176 - 01/28/09 04:17 PM (15 years, 2 months ago)

Trust me, it is not worth it for your day to day transfer work. I started with glass ones but I am so happy I switched to disposable ones now. A shipment of 100 pcs just arrived yesterday. Cost me 10 Euros plus 4 for shipping. 14 cents a dish is reasonable, because cleaning and sterilizing the glass ones every time costs energy and thus money, too. I only use glass lab supplies for anything I intend to do long term preservation and storage with. I use disposable units for everything else.


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

Extras: Filter Print Post Top
Offlinemushroomhunter10
Jack-Of-All-Trades
Male User Gallery


Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 8 years, 9 months
Re: Are my disposable petris sterile? [Re: German Kahuna]
    #9695395 - 01/28/09 04:50 PM (15 years, 2 months ago)

Wow... It seems that I've been REALLY lucky then!!

A thousand pardons.


So how would I reuse these dishes again? Would they withstand boiling?

Best Regards.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Need advice on sterility & trading spores. The_Gnome_King 4,385 16 06/25/20 05:22 AM
by Zakkery
* Every how often do i have to sterilize the misting water? Sterile 2,818 8 10/24/23 12:30 PM
by 10ftTall
* Re: Petri Dishes & Agar Q's Placebo 4,007 5 12/07/99 10:00 AM
by Anonymous
* How is the best way to pressure cook Petri Dishes?
( 1 2 all )
SafeHaven 16,194 20 12/09/23 10:27 PM
by vazap
* Do these myc petri look like they would benefit from cloning? Search59 509 4 11/19/04 05:48 PM
by Anno
* Serious side effects from improperly growing shroo crazyhorse 3,099 18 12/10/02 12:30 PM
by george castanza
* sterilizing petri dishes llamaboy 10,812 11 01/17/04 02:45 PM
by ragadinks
* Inoculating pasteurized poo nug into sterile bulk sub no-tone 2,216 7 04/14/04 10:42 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,618 topic views. 18 members, 115 guests and 79 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.012 seconds on 15 queries.