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he looka likaman
Registered: 10/14/02
Posts: 19
Last seen: 20 years, 1 month
Turn Your Bathroom Into A Sterile Clean Room.
    #967679 - 10/17/02 08:27 AM (20 years, 1 month ago)

Well, we all know that the most important part of success is having clean procedures. You can turn your bathroom into a sterile clean room during innoculation. By adhering to clean procedures, you should not have any problems with contamination.

bottle of Lysol antibacterial spray.
Spray bottle with 10% bleach and 90% water mixed into it.
Loads of paper towels
CLEAN Dust mask for face, along with hat/cap for hair and long sleeve shirt.

To prepare:
Close all airvents to the bathroom and draw curtain on your shower or close the shower door, wait about 10 minutes for dust to settle.
From here on out, any time that you are in the clean room make sure you are wearing a long sleeve shirt, a hat or cap on your head and a dust mask across your face. Make sure the long sleeve shirt, hat/cap and dust mask are clean, spray them down with lysol if you have to  :tongue:
I don't wear shoes, just clean socks.

Wipe down the area around the toilet with lysol, including the toilet itself, sink area, and floor underneath if you have tile.
After letting lysol sit for 30 seconds, wipe off with paper towels and lightly spritz with chlorine and water mixture.
Right before you open the jars, mist the air with the water/bleach mix and let it settle. Also do this anytime you enter the clean room with fresh jars, etc.
If your doing spore prints, dip your scraping instrument in rubbing alcohol and flame off before you use it to scrap spores. I also find this helpful for all instruments that touch the cake, including knives used to cut out any contamination, always flame off with alcohol.

To store:
Once you've been real sterile innoculating the jars, put lid on real tight and follow whatever Tek procedure you chose. Just make sure that whenever you open the jars you spritz the air with the mixture and put on a mask, that way you can be sure nothing is falling on your babies.

Edited by knellcast (10/17/02 08:36 AM)

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Registered: 05/25/02
Posts: 3,972
Re: Turn Your Bathroom Into A Sterile Clean Room. [Re: knellcast]
    #967703 - 10/17/02 08:34 AM (20 years, 1 month ago)

In reply to:

If using a spore print, dip in rubbing alcohol and flame off before you use it to scrap spores.

This sounds stupid to me, I think he means the knife or whatever (I use a teaspoon) that you are using to scrape the spores off.

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