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 DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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 Cake Cloning 
#9672908 - 01/24/09 07:49 PM (15 years, 2 months ago) |
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I dont know why more people dont do this...
Take either:
A. PF cakes that colonize quickly and are FULLY COLONIZED, or
B. Cakes that have shown prolific fruiting invitro, or
C.(riskier but works well!) A spent but successful cake.
Then take between 3 and 6 sterilized BRF jars, ready for inoculation. Sterilize the hell out of your work area, which should include a large, STERILE bowl. Lysol everything, your gloves, the outside of the jars you are working with, EVERYTHING.
Break up the cake you wish to clone into the smallest pieces you can, into the bowl. Empty the BRF jars with a spoon, one by one into the bowl. I set the verm in a pile on the side to go back on top of the jars. Mix everything so that the mycelium is evenly dispersed. Put back in jars, and this might take some practice working with sterilized brf as it is stickier and you are likely to make a mess if you havent got the technique down. Dont worry if it is a little uneven, a few crevices are actually nice bc it gives the cake more surface area to pin. And boy do they pin.
Place in incubating temps (mid eighties), and growth resumes instantly.
After one day, the little pieces will still be slightly bruised, but visibly growing. In 3-5 days the jars will be fully colonized and should fruit within 2 weeks.
The first time I tried this I thought it wouldnt work. I have done it with spent cakes, yet I HAVE NEVER LOST A JAR TO CONTAMS DOING THIS. It is amazingly fast, and you can expect high yields. I dont know why this is, but on most cakes the pinning occurs in large clumps, usually off of the top or the bottom. The cakes I have made this way pin all over the place. Like, never a flush with 5 big shrooms, always 30 or so immature ones. I still like to do my jars from spores, but I think I will use this tek for spent cakes that have impressed me. It is much quicker than cloning tissue for an LC, and it doesnt mean that you cant do that on top of it. It just means that you will have 5 jars of your prized strain fruiting while your LC jars are still colonizing.
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I think therefore I am not alone.
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veda_sticks
Cultivator
 
Registered: 07/29/07
Posts: 14,191
Loc: UK 
Last seen: 4 years, 3 months
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 Re: Cake Cloning [Re: DomiNero]
#9672919 - 01/24/09 07:52 PM (15 years, 2 months ago) |
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You can actually do a grain to grain kind thing with a pf cake. But it has to be freshly birthed in a sterile enviroment (glovebox,flowhood) to work.
You cant use a spent cake. The mycelium is old and weak, plus its covered in contaminants.
Another thing you can do, is put a fresh cake into a jar that had a fully colinised cake. There will be myceliam on the glass and that should colinise a fresh cake. There was a tek a while back about it.
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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Cloning tissue on LC - or agar - is a whole different animal.
When you clone a piece of mushroom tissue, you only clone the genetics of that particular fruit. In other words, only the strain(s) that produced that fruit, and you will be very likely to get those same characteristics in the next grows.
By "cloning" a whole cake, you clone all the different strains that are in a multispore-inoculated cake. In other words, it's not cloning at all. It's more like G2G but with cakes.
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
 Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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houdinihar
Adventurous Learner
Registered: 09/12/08
Posts: 1,111
Last seen: 4 years, 8 days
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i like this idea DomiNero and will definitely try that next time. maybe even try to expand into more substrate, just for grins.
thanks for the idea,
houdinihar
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Re: Cake Cloning [Re: dead]
#9673127 - 01/24/09 08:25 PM (15 years, 2 months ago) |
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First I would like to say I have been using spent cakes(I shave the outside off, but it might work without doing that), so to say it won't work is foolish; it does work. Second, this technique is very similar to G2G, but flowhood? Glovebox? I have been doing this for the past three months, I live in a studio, my bed and a couch with cat hair stuck on it are all in the same room as my ops, AND I HAVE YET TO LOSE ONE JAR THIS WAY. The f'ing things are done in 3 days, and like pretty much everyone else I use the most contam resistant strains.
Dead, I see your point, but a fully colonized cake from not too diverse a strain (i.e. the SAME SPORE PRINT) have already undergone anastamosis, and have traded off their nuclei. The cakes I get from this result are, like my LC's, identical.
Pisses me off to get ignorant comments from people who haven't even tried it, and probably never will because... I don't really know.
Anyway, if anyone does want to try this tonight, they could be eating muchos mas hongos in only a few short weeks...
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I think therefore I am not alone.
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monkeybus
Hare Krishna
Registered: 07/29/07
Posts: 521
Last seen: 2 years, 26 days
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Re: Cake Cloning [Re: DomiNero]
#9674262 - 01/25/09 01:19 AM (15 years, 2 months ago) |
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You really are better off just injecting some sterile water into a cake, giving it a shake, and suck it back up again. If you want to "clone" cakes, that would be far safer, and less work all around.
Still, to his own.
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Re: Cake Cloning [Re: monkeybus]
#9677503 - 01/25/09 04:26 PM (15 years, 2 months ago) |
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Quote:
monkeybus said:
You really are better off just injecting some sterile water into a cake, giving it a shake, and suck it back up again. If you want to "clone" cakes, that would be far safer, and less work all around.
Still, to his own.
I don't understand why you guys are having a hard time getting this.
So I have my syringe (which mind you will be more than likely contaminated), and then what? Do I inoculate a sterile BRF jar with it? That is risky and stupid as shit, that is what I would do to grow mold.
THIS IS NOT A TEK ON TISSUE CLONING. If I wanted a stable clone I would take a piece of tissue and throw it in a sterile jar with a dextrose/h2o solution. In fact that is what I do.
This is a tek to take one cake that's ready to fruit (or already fruiting), and turn it into a half dozen jars fruiting in about 2 weeks (or less!)
Show me another way to (with a delay of ONLY 2 WEEKS) increase your yield 500%.
I have been doing this for a few months and AM AMAZED at the results I am getting. You guys are all saying the stupidest shit, I don't know why you can't wrap your heads around this one.
Seriously, I haven't read advice this good on this board in years, and all I get is a bunch of idiots saying that it won't work or it is too much work! It takes less than 2 minutes to do this! Fucking retard!
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I think therefore I am not alone.
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States 
Last seen: 6 years, 1 month
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Re: Cake Cloning [Re: DomiNero]
#9677533 - 01/25/09 04:30 PM (15 years, 2 months ago) |
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You could stop being douchebag. That's generally the best way to get your point across.
Then again, you're yelling at people for ignoring your tek on PF Cakes, which most people grow out of in a month or two tops.
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: Cake Cloning [Re: Brennus]
#9677643 - 01/25/09 04:43 PM (15 years, 2 months ago) |
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I call bullshit.
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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rollinhard
I typically use search
Registered: 12/15/08
Posts: 71
Loc: state
Last seen: 9 years, 11 months
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Re: Cake Cloning [Re: DomiNero]
#9677707 - 01/25/09 04:50 PM (15 years, 2 months ago) |
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you could just us wbs jars, they are done in 10 days!! Thats less then 2 weeks!  makes syringes from that rinse and repeat.
Quote:
So I have my syringe (which mind you will be more than likely contaminated), and then what?
sterilize it?
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So what if I don't have a lot of post, I use the magical "search"!
Edited by rollinhard (01/25/09 04:54 PM)
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Re: Cake Cloning [Re: dead]
#9677743 - 01/25/09 04:55 PM (15 years, 2 months ago) |
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LOL BULLSHIT.
Try it, you will believe me in a few days.
PF cakes are probably what the majority of cubies are cultivated on. It isn't the highest yielder, but a shitload of people grow on cakes. And they're beautiful in their own right.
I am not trying to be a douche bag, but I am frustrated that I have posted a very useful and proven tek, that is useful to me (at least) and to anyone wanting to produce a volume of cakes, and people are telling me
A. it won't work.
B. try this crappy way of making an LC and quit wasting your time.
Shooting sterile water into a cake to get a culture sounds like it won't work, but I haven't tried it. But I can guarantee it isn't as fast as this tek.
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I think therefore I am not alone.
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Quote:
rollinhard said:
you could just us wbs jars, they are done in 10 days!! Thats less then 2 weeks! makes syringes from that rinse and repeat.
Quote:
So I have my syringe (which mind you will be more than likely contaminated), and then what?
sterilize it?
Are you kidding?
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I think therefore I am not alone.
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 Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Where is Tahoe when we need him...
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: Cake Cloning [Re: dead]
#9678038 - 01/25/09 05:30 PM (15 years, 2 months ago) |
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Quote:
Show me another way to (with a delay of ONLY 2 WEEKS) increase your yield 500%.
Spawning to bulk?
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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Jitsu
JKD Love
Registered: 11/12/08
Posts: 1,073
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Quote:
Premedman1 said:
^
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Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.
How I get my Pinsets
The Capabilities Of A Shotgun FC
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: Cake Cloning [Re: Jitsu]
#9678085 - 01/25/09 05:40 PM (15 years, 2 months ago) |
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--------------------
"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Re: Cake Cloning [Re: dead]
#9678095 - 01/25/09 05:41 PM (15 years, 2 months ago) |
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Quote:
dead said:
Where is Tahoe when we need him...
This tek works.
I am not telling you to do it, keep going with whatever you're doing. But to people growing on cakes this is a very useful tek. You misunderstood the post from the beginning. And I know about that 'other animal', and probably have much more experience streaking than you (judging from your first post. You're genetics lesson sucked, and you are crazy if you think that dikaryotes can't mate.
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I think therefore I am not alone.
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DomiNero
Stranger
Registered: 01/21/09
Posts: 41
Last seen: 15 years, 2 months
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Re: Cake Cloning [Re: dead]
#9678121 - 01/25/09 05:46 PM (15 years, 2 months ago) |
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Quote:
dead said:
Quote:
Show me another way to (with a delay of ONLY 2 WEEKS) increase your yield 500%.
Spawning to bulk?
Maybe the thread title isn't clear enough... This is a tek for cakes.;)
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I think therefore I am not alone.
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: Cake Cloning [Re: DomiNero]
#9678156 - 01/25/09 05:52 PM (15 years, 2 months ago) |
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Quote:
to people growing on cakes this is a very useful tek.
I disagree.
Quote:
You're genetics lesson sucked, and you are crazy if you think that dikaryotes can't mate.
When have I ever said that?
You're the one who needs a lesson.
Multispore is multispore. Each time you use MS to inoculate a substrate you end up hundreds of different strains on your substrate. Some of them may mate, some of them will be incompatible and cannot mate. Anyway in the best situation - if you're lucky - you'll have only compatible strains, and they will form a single organism. This organism acts as one, but it will still contain various strains with each having their own genetic information, and each fruit that it produces will be a product of different genetics.
Also there will be strains that are inable to fruit at all. Just mooching off your substrate, doing nothing.
When you clone from tissue, or better yet, isolate on agar, you avoid all of this.
There is just so much wrong with your "tek" I don't know where to start.
Spent cakes are spent. The mycelium is old and tired, and riddled with contams besides, using them to inoculate anything won't end well.
Your sterile procedures are inadequate. Just because you supposedly got away with it a few times doesn't mean it will fly in the long run. You can't just use normal cakes with only a verm barrier for a filter as G2G. Not to mention not using a glovebox or flowhood.
Also, your "tek" aside, your attitude sucks. You come here all high and mighty like, "if you don't like my tek go fuck yourselves". It's not a good way to make friends on the Shroomery.
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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