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Offlinebtronson
first timegrower
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Registered: 05/02/07
Posts: 159
Last seen: 13 years, 9 months
liquid culture question
    #9669271 - 01/24/09 10:09 AM (13 years, 10 months ago)

i have a liquid culture thats been sitting in my refrigerator for over a year now.  there are no signs of mold and the mycelium still looks good but is very dense from not being shaken.  do u think this is still viable for inoculation?  i want to make new liquid cultures from if into some 500ml erlenmeyer flasks.

on a side note i want to buy a magnetic stirrer but all the ones i see are like 120 bucks.  where can i find one on the cheap?


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Offlinemushrume man
Sadis Factory
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Registered: 11/19/08
Posts: 375
Loc: PNW
Last seen: 10 months, 10 days
Re: liquid culture question [Re: btronson]
    #9669407 - 01/24/09 11:15 AM (13 years, 10 months ago)

you could make a stirrer pretty cheap- you tube


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InvisibleIcky
Chrome Gnome
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Registered: 04/25/01
Posts: 1,535
Loc: Bacon Road
Re: liquid culture question [Re: btronson]
    #9669411 - 01/24/09 11:19 AM (13 years, 10 months ago)



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OfflineCaptain Caveman
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Registered: 01/13/09
Posts: 2,253
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Re: liquid culture question [Re: Icky]
    #9669684 - 01/24/09 03:01 PM (13 years, 10 months ago)

Just make your own. I made mine today, and it cost me $0.00


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"Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"


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Offlinejohnny_poke
Brownian movement


Registered: 12/04/08
Posts: 442
Loc: Europe
Last seen: 9 years, 2 months
Re: liquid culture question [Re: Captain Caveman]
    #9669753 - 01/24/09 03:41 PM (13 years, 10 months ago)

If the magnet, wire, motor and transformer fairy comes to visit I could make one for $0.00 too :smile:


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[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]


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Offlinebtronson
first timegrower
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Posts: 159
Last seen: 13 years, 9 months
Re: liquid culture question [Re: johnny_poke]
    #9670928 - 01/24/09 10:05 PM (13 years, 10 months ago)

i found one one ebay for cheap so i think ill go that way rather than make my own.  more importantly tho was my question about my liquid culture.  i plan on making more of it.  how long can these be viable if kept refrigerated?  i have it in a normal 1pt. canning jar with lid and filter disk i made from small sheets of material used to filter air ducts and it seemed to work well.

i remember reading a VERY good liquid culture technique, using honey / sugar in the raw, about a year ago.  does anyone have that handy so i can bookmark it perhaps?


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OfflineMephistophelian
Quasi Hob-Nobbery
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Registered: 08/15/08
Posts: 2,527
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Last seen: 4 months, 23 days
Re: liquid culture question [Re: btronson]
    #9670956 - 01/24/09 10:12 PM (13 years, 10 months ago)

Magnet- Old Harddrive
Motor- Fan (cost $15 if you had to buy new)
Transformer- $0.25 ripped out of phone/answering machine at thrift store.
Hold Glue- $0.25
Electrical Box- $13 (made it alot easier to stabilize and hold jars on top)

:thumbup: Made 6 of 'em, two for a fellow member.



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Master Slants Made Simple


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OfflineCaptain Caveman
Uh-oh!
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Registered: 01/13/09
Posts: 2,253
Last seen: 6 months, 4 days
Re: liquid culture question [Re: Mephistophelian]
    #9671112 - 01/24/09 10:42 PM (13 years, 10 months ago)

Im lazy, so I didnt even use glue. I actually used pore tape to hold the magnet in place. IT took me hours to find the sweet spot because the magnets I harvested from a HDD were kinda small compared to ones seen in the techs. Heres my first LC that I made this week with the Sugar in the Raw tech.


Heres the link...
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6378527


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"Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"


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OfflineSpongiform
Some Cow
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Registered: 08/23/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 7 years, 2 months
Trusted Cultivator
Re: liquid culture question [Re: Captain Caveman]
    #9671133 - 01/24/09 10:48 PM (13 years, 10 months ago)

I did a comparison project recently.

I'd just use plain old sugar in the raw.  1 packet per cup of water is fine.


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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.


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