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Offlineshaggyeg6si
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Registered: 12/29/08
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Last seen: 7 years, 1 month
TC incubation temps
    #9654396 - 01/21/09 09:10 PM (13 years, 3 months ago)

I have  4 jars in my incubator, and current temp is about 81 degrees. My 2 jars of brazil has colonized, but the treasure coast remains dormant. Its been about 11 days now, the brazil is moving along pretty quickly, except for my jar that became contaminated and has abruptly halted colonizing. What are the temps that you guys have had success with tc? This is bulk spawn on rye berries in quart jars. Should I just give up on the current jars and re-inoculate or should I just call it quits, and throw out said grain?


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OfflineMagic_Hobo
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Re: TC incubation temps [Re: shaggyeg6si]
    #9654639 - 01/21/09 09:44 PM (13 years, 3 months ago)

I incubate at 76F. It's the same for all cubes, bro. There must be something else wrong with your jars.


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: Magic_Hobo]
    #9654677 - 01/21/09 09:50 PM (13 years, 3 months ago)

So suggestions? Wait longer, re-inoculate, or dump and start over?


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Invisible5HR00M5N4K3
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Registered: 01/11/09
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Re: TC incubation temps [Re: Magic_Hobo]
    #9654690 - 01/21/09 09:51 PM (13 years, 3 months ago)

was it MS, g2g, LC?


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OfflineBuckeye Oysters
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Re: TC incubation temps [Re: shaggyeg6si]
    #9654696 - 01/21/09 09:52 PM (13 years, 3 months ago)

81F is too high.  High temps will stall and contaminate mycellium.  Get between 70-77F and give it some time.


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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OfflineRogerRabbitM
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Re: TC incubation temps [Re: Buckeye Oysters]
    #9654719 - 01/21/09 09:56 PM (13 years, 3 months ago)

There is no strain that requires higher or lower colonization temps.  Normal room temp of 75F work fine for P cubensis.
RR


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: RogerRabbit]
    #9654837 - 01/21/09 10:20 PM (13 years, 3 months ago)

I acquired the jars from a friend, and I think they are from a LC.


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Invisible5HR00M5N4K3
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Re: TC incubation temps [Re: shaggyeg6si]
    #9654903 - 01/21/09 10:32 PM (13 years, 3 months ago)

hmm.  LC usually spreads like the plague (the good plague), for me.  even at 81.  if its not growing, something is almost definitely 'wrong'.  i wouldn't re-inoculate jars that are behaving iffy.

i'd start over. :frown:


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: 5HR00M5N4K3]
    #9655118 - 01/21/09 11:04 PM (13 years, 3 months ago)

Well I've lowered temps in the incubator and gonna play the waiting game.


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: shaggyeg6si]
    #9657509 - 01/22/09 10:50 AM (13 years, 3 months ago)

Incubator is now down to 74.1 and things have just about exploded. The TC has no growth still, and I wanted to run the idea past you guys that have experience with this. I don't see it as a problem, but I'm no expert. How about I do a G2G transfer of my uncolonized jars from my currently blooming brazil? I know this cuts out growing the TC but at this point, I just don't see it happening. I rolled the TC jars around a bit just to see if there was any growth on the inner grains, and nothin. The good news is, I am not seeing any signs of contamination. I think it may be a good way to rescue the jars instead of them being a waste. I also think I can spare 2 tablespoons of grain out of a quart, haha.


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: shaggyeg6si]
    #9657705 - 01/22/09 11:46 AM (13 years, 3 months ago)

No one?


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InvisibleRoadkillM
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Re: TC incubation temps [Re: shaggyeg6si]
    #9657753 - 01/22/09 11:56 AM (13 years, 3 months ago)

Sounds like you got a bad spore syringe(of TC)...
sometimes spores just don't germinate.
It could have happened to any strain.

I'd ask for a replacement for the bad syringe if I was you.

side note:
81 degrees F. is what I set my incubators at, and I get the fastest growth from doing so.



tc


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Offlineiskinbash
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Re: TC incubation temps [Re: Roadkill]
    #9657785 - 01/22/09 12:04 PM (13 years, 3 months ago)

I wouldnt do a g2g transfer to a jar that hasnt shown any growth after being inoculated. I would just play it safe and make some new jars. Wouldnt want to contaminate you brazilians. But hey, if you feel comfortable, go for it!


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Invisiblemister
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Re: TC incubation temps [Re: iskinbash]
    #9657819 - 01/22/09 12:14 PM (13 years, 3 months ago)

I just did a Treasure coast grow and I had growth on rye in no more than 5 days for all my jars. Also an easy way to get your heat right in the incubator is to just by an aqarium heater from wal-mart that is pre-set at 79 degrees. They are the short stubby ones. Not too expensive, but keeps from having to play the guessing game with a dial.


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: mister]
    #9658132 - 01/22/09 01:09 PM (13 years, 3 months ago)

I already have 2 submersables, one a 50 watt and the other a 200. I have been playing around with temps and the growth has just grown exponentially from lowering incubation temps to the mid 70's. I don't think the jars are contaminated, but like I said, if dropping on a bit of mycelial grain from my brazil can resusitate the tc, then lets do it!


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: shaggyeg6si]
    #9658328 - 01/22/09 01:39 PM (13 years, 3 months ago)

Oos, not resusitate the TC, that's incorrect. Resusitate the grain, so it's not wasted, from the Brazil.


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Invisiblemister
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Re: TC incubation temps [Re: shaggyeg6si]
    #9658386 - 01/22/09 01:48 PM (13 years, 3 months ago)

But if the grain has bacteria in it, then you would be wasting your spawn from the Brazil. Just pitch and start a new set of jars if you want to do G2G.


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Offlineiskinbash
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Re: TC incubation temps [Re: mister]
    #9658408 - 01/22/09 01:51 PM (13 years, 3 months ago)

Quote:

mister said:
But if the grain has bacteria in it, then you would be wasting your spawn from the Brazil. Just pitch and start a new set of jars if you want to do G2G.




Thats what im saying. You cant know for sure, so why run the risk? There is a reason why your jars didnt colonize in the first place. Just make a couple new jars if you want to do g2g.


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Offlineshaggyeg6si
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Re: TC incubation temps [Re: iskinbash]
    #9658454 - 01/22/09 01:57 PM (13 years, 3 months ago)

I understand that they didn't colonize, but that doesn't necissarily mean it was the grain, it was the LC that held the TC mycelium. Like I said, I acquired these from a friend, so let me hit her up and see if hers are getting anything. I was going to do a G2G anyways, and do a half dozen using one jar to colonize, I don't think it would be a bad idea to at least see what happens. Experiment, right? I was going to start soaking my new grain tonight anyways and PC tomorrow night.


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Invisiblemister
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Re: TC incubation temps [Re: shaggyeg6si]
    #9658643 - 01/22/09 02:31 PM (13 years, 3 months ago)

If the LC had a contam, it is in you grain as well. Plus it is always risky transporting grain that is fresh and not colonized.


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