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Offlinegoose2r
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How many cc's in a 3lb spawn bag of millet? Shake or not?
    #9639594 - 01/19/09 04:53 PM (15 years, 11 days ago)

Howdy,

I have read many posts about how many cc's to use in 1lb and 5lb bags, but not for 3lb bags.  Often 5-10cc is suggested for 1lb and 40-60cc is suggested for 5lb.  I will be using a VERY thick LC (S.A.).  I assume 10-30cc is what most people use for 3lb bags of millet?

I'd like to limit the injections per bag.  I'd like to make only one injection per bag to limit chance of contams.  I can get a 30ml(cc) syringe with a #18 or #20 needle.  I find #14 and #16 needles are too big and greatly lessen the life time of injection ports.

Also on my mind is shaking after injection with LC.  When jars are injected with LC everyone says to shake.  But I don't read many people (or any?) shaking there bags after they inject LC.  Is that so you can see the mycelium (or contam) grow on the side of bag instead of dispersed thought the bag and the millet's core?

-goose2r


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OfflineABC
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: goose2r]
    #9639749 - 01/19/09 05:11 PM (15 years, 11 days ago)

I haven't read that. I thought that under 10cc is adequate for all bags. like 5 cc should be fine.

If you inoculate with LC it should be fine to shake. Spores should not be shaken, from what i've read.

Just massage it to spread it over a larger surface area. For the purpose of getting it to colonize faster, since it would be working from multiple areas.


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Offlinegoose2r
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: ABC]
    #9639904 - 01/19/09 05:27 PM (15 years, 11 days ago)

Thanks.

P.Menace uses 60ml for his 5lb spawn bags.  And I was under the impression that more spawn volume = higher inoculation amount (as long as the bags moisture isn't messed with).  I know a few other people I read use higher amounts but I don't have the threads bookmarked.

Spores shouldn't be shaken because they need to mate and shaking them moves them father apart.  But because a 3lb bag is a large volume of spawn if you inject into the center you may not notice a contam until weeks later when it makes it way to the sides.  If you inject on the side of the bag it allows you to check for contam that much faster, in like a few days, so your not wasting time on a bad bag .  But, the idea of shaking/massaging a bag AFTER there is a good amount of mycelial growth, say at 5-10% seems wise.  Then maybe shake/massage the bag one last time when it's at 40-50% colonized?

-goose2r


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OfflineABC
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: goose2r]
    #9641859 - 01/19/09 09:53 PM (15 years, 11 days ago)

Oh, I see. I did a little searching. The spawn volume will be the same anyway because the whole bag will colonize. The difference between more and less inoculant is just colonization time.

Either way (as long as it grows healthy), you'll end up with the same amount of grain spawn. Just it will take longer with less inoculant

Yea inoculate towards the side. Some people like to shake a lot, but I've read that success has been good with just one shake/massage at ~30% then just let fully colonize.

and then you'd massage to break it up right before you spawn it to bulk


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InvisibleShr00mZ
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: ABC]
    #9641930 - 01/19/09 10:00 PM (15 years, 11 days ago)

when using multispore do not shake.. because you want the spores to germinate and meet.. creating myc. (meaning the closer they are to eachother = faster results)
When using Liquid culture you want to shake so it spreads the live myc over the grains, that way you the grains can colonize quicker.

3-5 cc's is plenty for a 3 pound bag. Why use 1 syringe on 2 bags when you could use it on 4 bags?
1-3 cc's for 1 pound bags.
Dont shake after inocculation, let it sit at room temp. Turn u have snowy white myc covering 25% of ur grain. Then shake the grains apart mixing the already colonized grains with the uncolonized grains for faster colonization. Then you let it sit til 100%.


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Offlinegoose2r
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: Shr00mZ]
    #9646031 - 01/20/09 02:06 PM (15 years, 10 days ago)

Quote:

Shr00mZ said:
when using multispore do not shake.. because you want the spores to germinate and meet.. creating myc. (meaning the closer they are to eachother = faster results)

When using Liquid culture you want to shake so it spreads the live myc over the grains, that way you the grains can colonize quicker.

3-5 cc's is plenty for a 3 pound bag. Why use 1 syringe on 2 bags when you could use it on 4 bags?
1-3 cc's for 1 pound bags.

Dont shake after inocculation, let it sit at room temp. Turn u have snowy white myc covering 25% of ur grain. Then shake the grains apart mixing the already colonized grains with the uncolonized grains for faster colonization. Then you let it sit til 100%.




Thanks.  But I'm using a very thick LC, not MS.  I have over 2 pints of the LC so I don't mind using fair amount(10cc or more) in my 3lb bag if it will make the bag colonize faster without throwing off the moisture content of the bag.  I know using 5cc will work but I'd like to get faster colonization times than 5cc may provide.

My point about shaking/mixing the bag is about noticing contam more quickly vs. faster colonization time (from not mixing bag vs mixing the bag).

I think as I'm using LC its fine to inject and mix.  I'm gonna inject LC so it runs down the side of the bag and then I'll mix the bag.  I should still notice contams quickly because I'll let the LC run down the bag.

-goose2r


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Invisibleshroober
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: goose2r]
    #9646061 - 01/20/09 02:12 PM (15 years, 10 days ago)

to put this in perspective, I use 8-10 ccs of LC in each one of my quart sized jars, so pushing 10 ccs to a 3 pound bag will not be a problem

in my experience, the more LC you use the faster full colonization will occur, and people drastically over estimate the potentials of throwing off the water content, a few ccs will rarely have any type of negative impact on your water content assuming the jars are not already overly hydrated


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Invisibleshroober
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: shroober]
    #9646104 - 01/20/09 02:20 PM (15 years, 10 days ago)

that said, I wouldnt do more than 10ccs depending on the size of your syringes otherwise you will be doing multiple inoculations into the same bag which will increase your risk of contamination


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Offlinegoose2r
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: shroober]
    #9646162 - 01/20/09 02:35 PM (15 years, 10 days ago)

Yea thats kind of what I'm thinking.  I have a 30ml(cc) syringe.  I was originally thinking about injecting about 20cc but now I'm thinking about staying around 10-15cc.  I think a 3lb bag of WBS equals about 4-7 quarts of WBS (my guess).  Many people (including myself) use 1-2cc per quart.  If a 3lbs of WBS does come out to 4-7 quarts then  6-14cc in the 3lb bag would be about right.

I guess I need to weigh a quart of WBS to get a better idea.  Good info for figuring out spawn:sub figures too.

-goose2r


Edited by goose2r (01/20/09 02:36 PM)


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Offlinekjb1891
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: shroober]
    #9646319 - 01/20/09 03:03 PM (15 years, 10 days ago)

Hey Shroober, do you mix up your bags right after inoculating with LC? Or do you let it sit as is at first and wait for a good amount of colonization to take place before mixing it up?


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OfflineTreeMoss
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: kjb1891]
    #9646343 - 01/20/09 03:07 PM (15 years, 10 days ago)

Depends on your spore syringe.......if you make your own with a whole cap and break up the spores with a marble you get tons per CC.

But if it's a store bought with chunks of spores and things aren't all that even (which they usually have a good many per CC but not if you made your own).

Take that shit, put it in a jar of sterile water with potato dextrose with a sterile marble, give it mycelium temperature and swirl that marble around for a week then suck up a new syringe........way more spores spreading around........way more.......that is basically the keepers brew which isn't a bad idea just way to costly, way to costly to buy from there......but a great idea.


--------------------
Drug chemicals are going to be more abundant and survive longer than any anti-drug agendas.  Some of us are just ahead of the game, we already know what the future will understand.  Drugs weren't bad but how some people used them were and some people just were bad because they had to be.


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Invisibleshroober
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Re: How many cc's in a 3lb spawn bag of millet? Shake or not? [Re: kjb1891]
    #9646498 - 01/20/09 03:34 PM (15 years, 10 days ago)

Quote:

kjb1891 said:
Hey Shroober, do you mix up your bags right after inoculating with LC? Or do you let it sit as is at first and wait for a good amount of colonization to take place before mixing it up?




with LC i shake up immediately, then after about 25% colonization i'll shake once more.


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