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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: PE= potent as hell! [Re: Dreamster1]
#9607595 - 01/14/09 12:07 PM (15 years, 16 days ago) |
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Quote:
Dreamster1 said: grown outdoors?
no inside i did them in a monotub
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: PE= potent as hell! [Re: Workman]
#9608690 - 01/14/09 02:37 PM (15 years, 16 days ago) |
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I have been interested in this Psilocybe cubensis strain for a while and it seems that many of it's characteristics are similar to an unusual Psilocybe species found here in New Zealand! Unusual Psilocybe species! I am still uncertain wether this is a unique form of Psilocybe subaeruginosa or a different species altogether! Could it be possible that the PE strain is a partially secotioid form of Ps. cubensis?
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Spongiform
Some Cow


Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 4 months
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Re: PE= potent as hell! [Re: libertaire]
#9609141 - 01/14/09 03:42 PM (15 years, 16 days ago) |
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Quote:
I second this. You're going to be using a fruiting chamber of sorts to fruit the trays once they're fully colonized, so you would only be adding a step by doing this.
For noobies (like myself) I would just dunk and roll and fruit. Less added steps means less chance for contams.
I'd recommend (for noobs) to crumble into a simple monotub. The monotub is both the tray and the FC. Add some air holes and simple mist occasionally and they'll do fine. I've tested some very very simple setups and they fruit just fine and in decent quantities.
I crumbled about a pint worth of pf cakes into an empty plastic pie dish with a domed lid. It's done 3 flushes so far, each flush around 7-8 grams dry and it's fruiting it's 4th flush now.
I poked a few holes in the lid with a hot nail and every 2-3 days I take the lid off and mist it. After a flush I rehydrate with about an ounce of water.
In essence it's just a very simple/cheap monotub.
No fancy FC's, little/no maintenance, decent yields. I can't see how it gets any easier then that while still maintaining good yields.

The first flush, I just let them grow till they pushed the lid off. After that they started to dry out and bruise/turn a golden color. After that I just pick them as soon as they get big enough to mess with the lid. I end up with a bunch of short, thick meaty mushrooms. Still potent and dry weight is bout the same.
For a noob, I'd recommend a mini-monotub design.

If you're not spawning to bulk, you don't need the polyfill, just leave the holes open. Costs less then $10 to build one of these even with the fancy lid.
You can stick up to about 6 quarts worth of pf cakes in there if you want and still have room for the 'shrooms to grow nice and big. Can expect approx. 1 ounce dry per flush give or take a bit based on genetics.
-------------------- Spongiform's Plastic Tek - An Alternative to Glass Spongi's PF Block Tek & Automated Shotgun Project.
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
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Re: PE= potent as hell! [Re: Spongiform]
#9609178 - 01/14/09 03:50 PM (15 years, 16 days ago) |
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Quote:
Spongiform said:
Quote:
I second this. You're going to be using a fruiting chamber of sorts to fruit the trays once they're fully colonized, so you would only be adding a step by doing this.
For noobies (like myself) I would just dunk and roll and fruit. Less added steps means less chance for contams.
I'd recommend (for noobs) to crumble into a simple monotub. The monotub is both the tray and the FC. Add some air holes and simple mist occasionally and they'll do fine. I've tested some very very simple setups and they fruit just fine and in decent quantities.
I crumbled about a pint worth of pf cakes into an empty plastic pie dish with a domed lid. It's done 3 flushes so far, each flush around 7-8 grams dry and it's fruiting it's 4th flush now.
I poked a few holes in the lid with a hot nail and every 2-3 days I take the lid off and mist it. After a flush I rehydrate with about an ounce of water.
In essence it's just a very simple/cheap monotub.
No fancy FC's, little/no maintenance, decent yields. I can't see how it gets any easier then that while still maintaining good yields.

The first flush, I just let them grow till they pushed the lid off. After that they started to dry out and bruise/turn a golden color. After that I just pick them as soon as they get big enough to mess with the lid. I end up with a bunch of short, thick meaty mushrooms. Still potent and dry weight is bout the same.
For a noob, I'd recommend a mini-monotub design.

If you're not spawning to bulk, you don't need the polyfill, just leave the holes open. Costs less then $10 to build one of these even with the fancy lid.
You can stick up to about 6 quarts worth of pf cakes in there if you want and still have room for the 'shrooms to grow nice and big. Can expect approx. 1 ounce dry per flush give or take a bit based on genetics.
Do you crumble the cakes to anything? If not, I don't see what the point of crumbling them would be. Not only does it reduce the surface area by covering the bottom half of the mycelium with a tray, it also adds a good amount of time for the mycelium to recover from the crumble.
If you are crumbling it to a substrate, that is understandable, as it then expands the nutrients that the mycelium is getting, as well as the surface area that is available for fruiting, but for a noob, I still think this unnecessarily complicates things, and raises the likelihood that contamination can arise, even if you use the monotub setup. Just my opinion though.
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Spongiform
Some Cow


Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 4 months
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Re: PE= potent as hell! [Re: libertaire]
#9609222 - 01/14/09 03:57 PM (15 years, 16 days ago) |
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I've done both. I like to do the bulk subs but I've tried without too. The main difference is - with cakes alone you need a good FC with proper humidity. With a large cake (ie: monotub) it provides it's own humidity for the most part. There's none of this mist/fan 6-8 times a day stuff or working to maintain 99% RH.
Plus, an FC only holds so many cakes. You can stuff alot of cakes into a small tub. I'm not sure how much difference it makes having a larger surface area either. The total amount of nutrients available remains the same, although granted you'll lose some to repair due to crumbling.
You'll just get fewer, but larger/heavier mushrooms.
Maybe I'm totally missing something here - but isn't a small simple monotub less complicated then building a fruiting chamber?
I see the point about contams, but as long as your cakes are fully colonized it seems like the risk would be minimal.
-------------------- Spongiform's Plastic Tek - An Alternative to Glass Spongi's PF Block Tek & Automated Shotgun Project.
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: PE= potent as hell! [Re: Spongiform]
#9609271 - 01/14/09 04:08 PM (15 years, 16 days ago) |
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To compare PE trip characteristics with others....
I would describe it as giving you extra sensory perception or something of that sorts.
Every PE trip has been the same. Typically the visuals are not very profound. hearing is better, vision is better, hand eye coordination, etc...
The feeling of superman is pretty much in effect. Happy and being superman.
That's my take.
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Jean-Luc Picard
I only wish i was this good!



Registered: 12/30/07
Posts: 4,177
Loc: New Mexico, USA
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Re: PE= potent as hell! [Re: Liquidkick]
#9609359 - 01/14/09 04:30 PM (15 years, 16 days ago) |
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Well this has definitely renewed my interest in getting some petris going, I have one PE swab, and i need to get started on this since i have enough supplies to get 30 quarts or more of grain spawn and mix that into 30-40 lbs of hydrated hpoo...im excited now. I will begin working on this very soon...If it works i will buy a huge pack of sterile swabs and have a swab give away sometime in the next 6 months. 
-------------------- The universe is under no obligation to make sense to you - NDT
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iMtRiPpIn
Lookin' fer Luv



Registered: 06/04/08
Posts: 530
Last seen: 11 years, 3 months
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Re: PE= potent as hell! [Re: 13shrooms]
#9609548 - 01/14/09 05:03 PM (15 years, 16 days ago) |
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Quote:
13shrooms said: can n e 1 tell me what vendor their potent PE strain came from? chances are they could b still the same strain.
The Hawk's Eye has some kick-ass PE.
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: PE= potent as hell! [Re: 13shrooms]
#9609924 - 01/14/09 05:59 PM (15 years, 16 days ago) |
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when i was studying this:
Sporeworks Genetics from 10/03 Lot: R-XXI
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
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Re: PE= potent as hell! [Re: Spongiform]
#9609999 - 01/14/09 06:07 PM (15 years, 16 days ago) |
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Quote:
Spongiform said: I've done both. I like to do the bulk subs but I've tried without too. The main difference is - with cakes alone you need a good FC with proper humidity. With a large cake (ie: monotub) it provides it's own humidity for the most part. There's none of this mist/fan 6-8 times a day stuff or working to maintain 99% RH.
Plus, an FC only holds so many cakes. You can stuff alot of cakes into a small tub. I'm not sure how much difference it makes having a larger surface area either. The total amount of nutrients available remains the same, although granted you'll lose some to repair due to crumbling.
You'll just get fewer, but larger/heavier mushrooms.
Maybe I'm totally missing something here - but isn't a small simple monotub less complicated then building a fruiting chamber?
I see the point about contams, but as long as your cakes are fully colonized it seems like the risk would be minimal.
Point taken. If you're careful, a small monotub can be straight ahead and a good thing for a first try...if you're careful! I'm actually planning on making a monotub fruiting chamber like this gentleman did, so that my second attempt I can just convert it into an actual mini-monotub. Maybe if I'm lucky I'll get my hands on some PE and find out about this legendary strain for myself. ::trying to keep the thread on topic::
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Dreamster1
Old School



Registered: 11/11/08
Posts: 258
Last seen: 6 years, 2 days
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Re: PE= potent as hell! [Re: Workman]
#9613667 - 01/15/09 10:14 AM (15 years, 15 days ago) |
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Hey Workman! I was hoping you would stumble onto this thread! I will definitely send you a PM to catch up! I am so glad that Sporeworks is doing so well - you were always a very helpful, humble and honest guy. It is very good to see your still a prominent figure on the Shroomery. Also really glad that the PE strain, as it is now coined, is a prominent choice amongst hobbiest. Pretty neat you still have that original sample - I actually LOST the original print that I had...I know i know that is inexcusable! But at least a piece of it is in good hands!
Cheers!
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Spongiform
Some Cow


Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 4 months
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Re: PE= potent as hell! [Re: Dreamster1]
#9616060 - 01/15/09 05:20 PM (15 years, 15 days ago) |
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So, I just finished getting my next grow ready. I only have a 6qt presto so this took me a few days.
Everything is inoculated with a PE cloned LC.

Top shelf is PF with coffee/gypsum added. Rest is straight WBS (with gypsum added)
-------------------- Spongiform's Plastic Tek - An Alternative to Glass Spongi's PF Block Tek & Automated Shotgun Project.
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Dreamster1
Old School



Registered: 11/11/08
Posts: 258
Last seen: 6 years, 2 days
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Re: PE= potent as hell! [Re: Spongiform]
#9616126 - 01/15/09 05:29 PM (15 years, 15 days ago) |
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wow! even with a few jars losing out to green mold you could have a forest of PE's going soon! nice work are you going to spawn to straw/hpoo? curious what method you used for liquid culture.
looks great! 5 shrooms for you
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Spongiform
Some Cow


Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 4 months
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Re: PE= potent as hell! [Re: Dreamster1]
#9616166 - 01/15/09 05:35 PM (15 years, 15 days ago) |
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I did an LC comparison recently.
I'll primarily be sticking with light malt extract. It's insane stuff.
Yeah I expect a few to drop out to contams since I don't use a glovebox or anything. I'm gonna start another batch of wbs next week when I get around to it.
I'll be spawning to a variety of subs/mixes/different size monotubs.
I've got enough hpoo for about 6 full size monotubs on hand. I'm gonna pick up some hay or straw soon as I can. I've got a big bag of corn cob bedding to play with.
And I want to experiment with some stuff like composted leaves and MGMC. I picked up a bag to use as casing mix and after reading the ingredients, it sounds like a perfect bulk sub.
Peat Moss, Coco Coir, Compost, Perlite + some additives. I used a little as casing mix and my tubs have been eating it up like its candy.
-------------------- Spongiform's Plastic Tek - An Alternative to Glass Spongi's PF Block Tek & Automated Shotgun Project.
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Dreamster1
Old School



Registered: 11/11/08
Posts: 258
Last seen: 6 years, 2 days
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Re: PE= potent as hell! [Re: Spongiform]
#9620955 - 01/16/09 12:25 PM (15 years, 14 days ago) |
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awesome. i have never tried liquid culture. i am rather old school in my methods - in fact i am just doing my first ever multi-spore PE grow now. at first it didn't look to promising but they have turned up the pace quite nicely in the last few days. just a simple spore-to-grain-to-straw with a peat/verm/calcium carbonate casing. straw depth 8". got a few rouge pins that i just let alone figuring a low yield with multispore but now the casing is covered with pins. i just cant say enough abut this strain. one thing i read alot on these boards is that it is low yielding - i have never known that to be the case. in fact it usually is quite prolific. in years past i have reached a consistent 150-200% BE with it
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
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Re: PE= potent as hell! [Re: Dreamster1]
#9620989 - 01/16/09 12:33 PM (15 years, 14 days ago) |
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Sorry, I'm a bit of a noob....BE?
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Dreamster1
Old School



Registered: 11/11/08
Posts: 258
Last seen: 6 years, 2 days
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Re: PE= potent as hell! [Re: libertaire]
#9621083 - 01/16/09 12:49 PM (15 years, 14 days ago) |
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Biological Efficiency
The definition of biological efficiency (BE) in mushroom cultivation is: 1 pound fresh mushrooms from 1 pound dry Substrate indicates 100% biological efficiency.
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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.
Edited by Alabama Slim (01/30/12 04:24 PM)
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Dreamster1
Old School



Registered: 11/11/08
Posts: 258
Last seen: 6 years, 2 days
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No, just my first PE multi-spore grow. Like Workman noted, I have been cultivating this strain since at least 1997 - but I had many grows under my belt before I sent that sample to him so prolly more like 1995 so that's at least 14 years. WOW sounds long when I actually type it out hahaa...
As for my method with this strain. For indoor grows I would just isolate properly on agar then spawn to rye grain then transfer to straw. I actually got better results with straw then hpoo indoors. I never use straw outdoors - always hpoo. this strain really thrives outdoors.
Anyhow, my indoor methods were very low tech and simple: I would line a milk crate with black plastic and then "layer/spawn" my straw and grain until about a bit more than half of the crate was full. I would then cover with clear plastic for 10 days. Then apply casing made up of peat/verm/calcium carbonate with a bit of gypsum. About 1/2 -3/4" inch thick. Re-cover with clear plastic for about three days or until mycelium poked through. Then I would mist and fan lightly in the morning and evening, leaving the plastic on between misting. About two weeks into this I would poke holes in the bottom of the plastic bag and let any excess water drain out.
I never used any humidifiers, fans or lights. These crates were I guess that eras version of the mono-tub. But no need for that silly poly-fill in the holes, etc. Just cover the thing like I described and when its time for fruiting just mist and fan gently. No temp drops, special light sequencing, blah blah. I would fan between misting's from time to time as well. Biggest red flag for CO2 build-up were elongated stems. Cap appearance is kind of useless indicator with this strain as the cap is ususally (indoors atleast) very underdeveloped.
I hardly EVER lost anything to contamination. I used a laminar flow hood for agar work, spawn generation, etc. But I spawned the crates in less than sterile air. I also never treated my casing with anything either - just mixed, moistened and applied. Occasionally I would get fungus gnats. They did little harm to the mushrooms but annoyed the hell out of me so I started treating my casing with a bit of steam to knock them out of the picture.
Then I would dry them and take 5 grams out to my favorite spot by the river, build a fire and voyage! The thing (staying on-topic for this thread haha) about the vibe this strain always brought me: VERY VERY spiritual. I would consistently make contact with spirits - Native American, Mayan, etc. I learned many songs from this mushroom as well. Songs I can chant and sing at anytime now even without ingesting the mushroom. I once ate 8 dried grams outdoors on an island at night and clearly conjured up alien visitors. My two friends with me at the time could see them too and kept saying "they want to talk to the one who grows the mushroom". That was the most intense experience of my life - I never went over 5 grams before and haven't again since. Well, with PE strain that is. Other Cubes I can easily handle a ten to twelve gram ride. This one is, contrary to popular public opinion that a cube is a cube, is definitely in a class all its own. Use them wisely.
Cheers!
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xswaveyx
SwaveY



Registered: 08/22/08
Posts: 1,077
Loc: East
Last seen: 2 years, 11 months
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Re: PE= potent as hell! [Re: Spongiform]
#9625875 - 01/17/09 11:32 AM (15 years, 13 days ago) |
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Quote:
Spongiform said: So, I just finished getting my next grow ready. I only have a 6qt presto so this took me a few days.
Everything is inoculated with a PE cloned LC.

Top shelf is PF with coffee/gypsum added. Rest is straight WBS (with gypsum added)
Did you pressure cook those rubbermades? I never even thought of that... i guess it would work since mycobags dont melt.hmm.
-------------------- Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different. My Growlog with Redboy,Amazon,and Brazilian
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