|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9575197 - 01/09/09 01:25 AM (15 years, 2 months ago) |
|
|
I think it would last quite a while, though obviously not as long as one with GE.
I wouldn't use LC's for long term storage anyhow... actually I'm thinking of scrapping LC's all together in favor of cultures on agar and master culture slants.
Edit, realizing this post was almost useless to OP, I'd like to add the idea of airport syringes, take a normal sterile syringe and some sterile polyfil or filter disk material and stuff it in the syringe (take out plunger) now stick this in to do filtered air while sticking the 2nd syringe in to do spores, etc. You can also suck out stale air with one while leaving the airport in.
Edited by Shroominit (01/09/09 01:37 AM)
|
German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9575231 - 01/09/09 01:39 AM (15 years, 2 months ago) |
|
|
For long term storage I agree on the slants and agar, actually. But I like the colonization speed of my jars when I use LC to noc them up.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: German Kahuna]
#9575235 - 01/09/09 01:41 AM (15 years, 2 months ago) |
|
|
Agar wedge to grain LC? or just agar wedge to grain? followed by G2G's I heard was much much faster (haven't tested that yet).
|
German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9575259 - 01/09/09 01:52 AM (15 years, 2 months ago) |
|
|
Well, it certainly is much faster if you have to drop the wedge into an LC jar first and wait for the LC to grow out before you noc up your jars, but I am not sure if the wedge to grain is really faster if you already have an LC ready, because injecting live mycelium in a liquid and then shaking it up will give it a kick start at many different spots in the jar. I have to admit that I haven't tried agar wedges to grain directly, yet, so I am speculating. I can really only make a first hand comparison between inoculation from a spore syringe and LC, and of course LC beats the spore syringes hands down in that race. I didn't have to be a rocket scientist to figure that one out either.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: German Kahuna]
#9575291 - 01/09/09 02:04 AM (15 years, 2 months ago) |
|
|
I will do a comparison run in a about a month here when I get everything situated. I know LC in grain is quick because of so many pieces of myc being spread around, so I figure that may be faster than agar -> grain. I think G2G might be as fast as LC though. I'll start one of each when I have all three ready to go. All from the same master culture to make it even, of course no MS for this.
|
The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 6 months, 24 days
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9575330 - 01/09/09 02:16 AM (15 years, 2 months ago) |
|
|
Quote:
Shroominit said: Agar wedge to grain LC? or just agar wedge to grain? followed by G2G's I heard was much much faster (haven't tested that yet).
Agar wedge to grain, and then G2G. That is my preferred method. I'm doing a grow log of Penis Envy that will showcase that method. Should be in my journal by Monday if all goes well. By the way Shroominit, that did make a lot of sense about using a syringe of sterile water to release the vacuum. I hadn't even thought of that.
The Shroomy 1
-------------------- AMU Q&A thread.
|
4 ho cultivar
Transcending plains
Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 15 years, 1 month
|
Re: help!! injected too much into culture!!! [Re: The shroomy 1]
#9575382 - 01/09/09 02:38 AM (15 years, 2 months ago) |
|
|
o.k. first off boneboy1 posted this question/thread not shroom it one. I'm guilty of thread jacking but this is blatant. Hey boneyboy what you should do is wait 7 days if no myc....{which there should be by now}..... if no other options and it was me. I would p.c. a 10ml syringe with 1ml water in it at 10 psi 25 min 15 psi at 20 min. {boil water suck up and blow out 3x blow out suck up 1 ml/cc then hold need up and suck in all air. wrap in alum foil really good. p.c.} now in a glove box make super sterile, do your absolute best. unwrap alum foil inject 10cc air wait 7 more days. after 14 days if you don't have l.c. then you can.................suck it. o.k suck! IT! Why suck it ...... Because you caused me carpal tunnel.... you and only you. die!!!! I hope this helps. peace
-------------------- :Patience be thy virtue Blurring the line between reality and dreams Mr. Winkie says: "Procrastinators unite................................tommorrow!! G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!"
Edited by 4 ho cultivar (01/09/09 02:39 AM)
|
veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
|
Re: help!! injected too much into culture!!! [Re: 4 ho cultivar]
#9575904 - 01/09/09 08:13 AM (15 years, 2 months ago) |
|
|
Thats totally overkill for sterilising a syringe. Boiling water for 10-20 minutes, then sucking up the syringe full, waiting 2 minutes the emptying the syringe (not back into the pot) and repeat 3 or 4 times will clean out a syringe.
If you pressure cook it, that will definatly sterilise it, no need to fill with boiling water 3 times.
I have read a tek on LC that only mentioned 1 self healing injection point, but that tek did warn about the vacum and also needing to inject sterile air.
Personaly i like the idea of only having 1 hole thats sealed and its probably the way ill make my next LC. No risk of getting your filter wet.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
|
4 ho cultivar
Transcending plains
Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 15 years, 1 month
|
Re: help!! injected too much into culture!!! [Re: veda_sticks]
#9577961 - 01/09/09 04:30 PM (15 years, 2 months ago) |
|
|
o.k. don't suck it up 3-4x. your right that would take forever. just suck up at least 1ml and pull 10ml syringe open.
-------------------- :Patience be thy virtue Blurring the line between reality and dreams Mr. Winkie says: "Procrastinators unite................................tommorrow!! G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!"
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: 4 ho cultivar]
#9578165 - 01/09/09 05:02 PM (15 years, 2 months ago) |
|
|
Quote:
4 ho cultivar said: o.k. don't suck it up 3-4x. your right that would take forever. just suck up at least 1ml and pull 10ml syringe open.
4 ho, Stop posting, you're not adding anything valuable to the thread.
veda, I loved the one sealed hole LC's, felt like I had freedom to shake and stir all I wanted without contam-ing it.
|
fishfarmin
Predator Hunter
Registered: 09/04/08
Posts: 196
Last seen: 14 years, 11 months
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9578438 - 01/09/09 05:43 PM (15 years, 2 months ago) |
|
|
i've never had a vacuum problem.if your jar is allowed the time to reach room temp inside the pc.the only time a vacuum is created is when then lid is tightened on a hot jar,or when you remove lc without replacing the the liquid with a syringe full of sterile water.i always sterilizethe water syringes i intend to use for lc at the same time i do my lc jars.when the lc is mature then express the water in the jar and remove lc.
:my2cents:
-------------------- "Government exist to protect us from each other.Where government has gone beyond its limits is in deciding to protect us from ourselves." Ronald Reagan "It is error alone which needs the support of government.Truth can stand by itself."Thomas Jefferson "It has been my experience that folks who have no vices have very few virtues."Abraham Lincoln
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: fishfarmin]
#9578498 - 01/09/09 05:53 PM (15 years, 2 months ago) |
|
|
I posted that info in this thread earlier: http://www.shroomery.org/forums/showflat.php/Number/9575140#Post9575140
If you wait for your jar to cool, it's gonna suck contams in from the sides of the lid. I would reccomend against it, if you can.
|
fishfarmin
Predator Hunter
Registered: 09/04/08
Posts: 196
Last seen: 14 years, 11 months
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9579210 - 01/09/09 07:46 PM (15 years, 2 months ago) |
|
|
how can it get contams if it is allowed to cool inside a sterile pc?
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: fishfarmin]
#9579214 - 01/09/09 07:48 PM (15 years, 2 months ago) |
|
|
Quote:
fishfarmin said: how can it get contams if it is allowed to cool inside a sterile pc?
As air cools, it becomes more dense, meaning the air inside the PC is only gonna fill about 1/3 to 1/2 of the PC... it will then suck in 'dirty' air which can fly right into your jars.
I'm not saying its imminent, I am saying that it's possible though.
|
fishfarmin
Predator Hunter
Registered: 09/04/08
Posts: 196
Last seen: 14 years, 11 months
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9579235 - 01/09/09 07:52 PM (15 years, 2 months ago) |
|
|
never had that happen,suppose it is possible though.i guess it depends on the pc you use:)
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: fishfarmin]
#9579258 - 01/09/09 07:55 PM (15 years, 2 months ago) |
|
|
You don't see it happen, it is how your PC gets back to normal pressure =/
If you mean your jars have never contam'd then I'd say your air is pretty clean in your house/grow room.
|
MushHunter08
Mycological Pupil
Registered: 06/08/08
Posts: 522
Loc: B.F.E
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9579307 - 01/09/09 08:00 PM (15 years, 2 months ago) |
|
|
Boneboy exactly what LC tek did you use to create your LC? Also, after you inoculated the jars, how off does the moisture content look to you? Does it looked drenched in the one you dumped 10cc's into?
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
|
fishfarmin
Predator Hunter
Registered: 09/04/08
Posts: 196
Last seen: 14 years, 11 months
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9579310 - 01/09/09 08:00 PM (15 years, 2 months ago) |
|
|
i have had my share of contams.i've kept a hepa filter and negative ion generator running 24/7 in my room for about 6 years.it keeps contams almost completely nonexistant.that and the use of a flow hood.
|
Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
|
Re: help!! injected too much into culture!!! [Re: MushHunter08]
#9579588 - 01/09/09 08:35 PM (15 years, 2 months ago) |
|
|
Quote:
MushHunter08 said: Boneboy exactly what LC tek did you use to create your LC? Also, after you inoculated the jars, how off does the moisture content look to you? Does it looked drenched in the one you dumped 10cc's into?
Well the LC jar ought to look fucking wet considering its a LIQUID CULTURE.
Quote:
fishfarmin said: i have had my share of contams.i've kept a hepa filter and negative ion generator running 24/7 in my room for about 6 years.it keeps contams almost completely nonexistant.that and the use of a flow hood.
So since you've had your fair share, what's to say you didn't get a contam while your jars were cooling in the pressure cooker? There's no way to tell.. I'm just saying that it's possible.
|
MushHunter08
Mycological Pupil
Registered: 06/08/08
Posts: 522
Loc: B.F.E
|
Re: help!! injected too much into culture!!! [Re: Shroominit]
#9582777 - 01/10/09 12:46 PM (15 years, 2 months ago) |
|
|
Quote:
Shroominit said:
Quote:
MushHunter08 said: Boneboy exactly what LC tek did you use to create your LC? Also, after you inoculated the jars, how off does the moisture content look to you? Does it looked drenched in the one you dumped 10cc's into?
Well the LC jar ought to look fucking wet considering its a LIQUID CULTURE.
Wow don't get hostile. I misunderstood. I thought he was knocking up PF jars w/ the LC not just knocking up an LC. If that's the case only time will tell as mentioned already. Let it run it's course and just try and be more careful next time while you are inoculating anything. Trial and error is what its all about. To error is human after all.
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
|
|