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Offlineboneboy1
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help!! injected too much into culture!!!
    #9574324 - 01/08/09 10:19 PM (15 years, 2 months ago)

Ok this is my first time using liquid culture.. I just when to inoculate the jars and forgot about the vacuum inside the jars of culture...=/... Ended up with about 10cc in one and about 5 in the 2nd... is this bad???

Thank you

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Invisibleslacker008
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574336 - 01/08/09 10:21 PM (15 years, 2 months ago)

First off, I am GUESSING your doing half pints? If so, 10cc is bound to drown them but at this point, theres nothing you can do but wait and pray.

5 might be better off.

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Offlineboneboy1
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Re: help!! injected too much into culture!!! [Re: slacker008]
    #9574411 - 01/08/09 10:32 PM (15 years, 2 months ago)

they are 500mL jars.. and also... do i eleased and introduce oxygen into the jar right after i add the spores..

thanks
sam

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OfflineBoris
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574434 - 01/08/09 10:37 PM (15 years, 2 months ago)

Quote:

boneboy1 said:
they are 500mL jars.. and also... do i eleased and introduce oxygen into the jar right after i add the spores..

thanks
sam




You need gastric air holes, not holes big enough for fresh air exchange.


--------------------
AMU

Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."

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OfflineThe shroomy 1
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574447 - 01/08/09 10:40 PM (15 years, 2 months ago)

I'm kind of confused.  You must have not prepared the lid correctly.  You should have an injection port and a gas exchange hole.  That second, (GE), hole would have prevented the vacuum.

The Shroomy 1


--------------------


AMU Q&A thread.

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Offlineboneboy1
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Re: help!! injected too much into culture!!! [Re: The shroomy 1]
    #9574492 - 01/08/09 10:49 PM (15 years, 2 months ago)

there is only one hole with a rubber seal..

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Offlineboneboy1
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574517 - 01/08/09 10:55 PM (15 years, 2 months ago)

so do i let sterile air into the jar right after i put in spores??

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OfflineThe shroomy 1
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574520 - 01/08/09 10:55 PM (15 years, 2 months ago)

Check out this thread.  You might get some ideas for future LC's https://www.shroomery.org/forums/showflat.php/Number/6553274#6553274

The Shroomy 1


--------------------


AMU Q&A thread.

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OfflineThe shroomy 1
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574530 - 01/08/09 10:58 PM (15 years, 2 months ago)

Quote:

boneboy1 said:
so do i let sterile air into the jar right after i put in spores??




I don't know what to tell you.  If it was me, I would start over.  I'm sure you still have some spores right?  Do a little more research and start again.

The Shroomy 1


--------------------


AMU Q&A thread.

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OfflineBoris
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9574619 - 01/08/09 11:17 PM (15 years, 2 months ago)

Quote:

boneboy1 said:
so do i let sterile air into the jar right after i put in spores??




By doing this something tells me you mean open the lid and fan some air in the jar. :lol: By any means, don't open the jar. Contaminants will take a nose dive straight in your jar.


--------------------
AMU

Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."

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Offlineboneboy1
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Re: help!! injected too much into culture!!! [Re: Boris]
    #9575006 - 01/09/09 12:32 AM (15 years, 2 months ago)

no i didn't open the lid.. lol i flamed a 10cc needle and slowly sucked in 10cc of air over the red hot needle, hopefully making 10cc of sterile air..

SAM

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OfflineKnifeplay
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9575024 - 01/09/09 12:36 AM (15 years, 2 months ago)

Simple solution get a syringe with as big of a gauge as you can. Open it up and put pollyfil  in it. Plug it in, next time try to do some more reading before you venture into something. It will save you a lot of trouble.


--------------------
bmiles: shrooms are 90% cum.

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InvisibleShroominit
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Re: help!! injected too much into culture!!! [Re: Knifeplay]
    #9575075 - 01/09/09 12:51 AM (15 years, 2 months ago)

Okay, don't know how many people missed it, but he's doing an LC.

boneboy, you don't need GE or FAE in the short run for your LC. Just shake it up REALLY well every day for a few days. As long as the LC isn't carmelized, you should be good (even then you should be OK, really)

If you get good myc growing, knock up ONE grain jar and wait a few days (3-4) to make sure it's clean. Then go to town with the LC or G2G's.

Good luck!

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InvisibleGerman Kahuna
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Re: help!! injected too much into culture!!! [Re: boneboy1]
    #9575091 - 01/09/09 12:56 AM (15 years, 2 months ago)

Start over and do some research first so you do it properly the next time.
What you call "sterile air" is achieved by putting a strip of micropore tape over the GE hole. I also use a piece of aluminum foil as a rather loosely applied lid working as an additional dust barrier.


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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OfflineThe shroomy 1
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Re: help!! injected too much into culture!!! [Re: Shroominit]
    #9575109 - 01/09/09 01:00 AM (15 years, 2 months ago)

Quote:

Shroominit said:
Okay, don't know how many people missed it, but he's doing an LC.

boneboy, you don't need GE or FAE in the short run for your LC. Just shake it up REALLY well every day for a few days. As long as the LC isn't carmelized, you should be good (even then you should be OK, really)

If you get good myc growing, knock up ONE grain jar and wait a few days (3-4) to make sure it's clean. Then go to town with the LC or G2G's.

Good luck!




No disrespect, but  Every LC tek I've currently seen out there, dictates 2 holes in the lid.  One for injection, and one for Gas Exchange.  His problem occurred because he was missing a hole for GE.  You don't want a vacuum to be created in a LC jar.  When he put the syringe in the hole, the vacuum sucked the contents of the syringe into the jar. When the LC is ready, you just stick the sterile syringe in the injection port and draw in. 

The Shroomy 1


--------------------


AMU Q&A thread.

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InvisibleGerman Kahuna
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Re: help!! injected too much into culture!!! [Re: Shroominit]
    #9575120 - 01/09/09 01:03 AM (15 years, 2 months ago)

Quote:

The shroomy 1 said:
No disrespect, but  Every LC tek I've currently seen out there, dictates 2 holes in the lid.  One for injection, and one for Gas Exchange.  His problem occurred because he was missing a hole for GE.  You don't want a vacuum to be created in a LC jar.  When he put the syringe in the hole, the vacuum sucked the contents of the syringe into the jar. When the LC is ready, you just stick the sterile syringe in the injection port and draw in. 

The Shroomy 1




--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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InvisibleShroominit
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Re: help!! injected too much into culture!!! [Re: German Kahuna]
    #9575140 - 01/09/09 01:09 AM (15 years, 2 months ago)

Shroomy, I agree, to a point. When I make mine, I sterilize syringes full of water and de-presurize them with the sterile water.

Once the pressure is gone, you don't need (a lot of) GE. For an LC tek with no GE try the baby bottle tek.

--Tested on two jars, personally. This is purely my experience.
I didn't give my jars any GE and they colonized about 50%, which is not to say the myc won't die without O2, it probably will. Just from personal experience with pint jars (I think that's what he's got) that were 2/3 full, I could PC and shake, depresurize, and knock up then allow to grow without an issue.

I appreciate the concern for truthful information and I would not post this if I didn't think it was correct.

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InvisibleGerman Kahuna
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Re: help!! injected too much into culture!!! [Re: Shroominit]
    #9575169 - 01/09/09 01:16 AM (15 years, 2 months ago)

What I am asking myself is how long the LC would stay viable without any GE.
What I am also asking myself is when RR is going to stop by and bitchslap us all for talking crap... :biggrin:


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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InvisibleShroominit
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Re: help!! injected too much into culture!!! [Re: German Kahuna]
    #9575175 - 01/09/09 01:18 AM (15 years, 2 months ago)

Quote:

German Kahuna said:
What I am asking myself is how long the LC would stay viable without any GE.




Mine was thriving after two weeks un-refridgerated. If you wanted to do sterile air exchange, that would work too I guess, but it should stay viable long enough to knock up grain then toss it in the fridge. Or you could PC a quart jar that was like 1/2 full and transfer some LC to it.

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InvisibleGerman Kahuna
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Re: help!! injected too much into culture!!! [Re: Shroominit]
    #9575182 - 01/09/09 01:20 AM (15 years, 2 months ago)

Yeah, I was talking more in terms of long term storage, not weeks.


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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