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InvisibleMisterMuscaria
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Shiitake fruiting invitro
    #9568571 - 01/08/09 12:22 AM (15 years, 2 months ago)

I recently made a trade with a member for some cultures and among them was a large jar of shiitake on sawdust. Ive never grown this species (succesfully) and Im wondering what my options are for fruiting these. There are several large knots forming inside of the jar. I originally planned on transferring bits of the sawdust to other jars to colonize more sawdust but it looks like my sawdust source is being unresponsive and the mushrooms are not.
Any tips as to how I can get the biggest yield possible out of this jar would be appreciated.

Edited by MisterMuscaria (01/08/09 12:26 AM)

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Offlinemycoharry
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Re: Shiitake fruiting invitro [Re: MisterMuscaria]
    #9568601 - 01/08/09 12:27 AM (15 years, 2 months ago)

go watch RR's vidios


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we create realitys in our minds based on our own experiences, rather than fact. my experiences in life have been insane, so in reality i am in fact, INSANE...
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InvisibleMisterMuscaria
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Re: Shiitake fruiting invitro [Re: mycoharry]
    #9568614 - 01/08/09 12:30 AM (15 years, 2 months ago)

Ive seen his videos(the parts freely available on the internet), and Ive experienced no difficulty with cubes, the pf tek, etc.

Im just wondering about possible uses of the colonized sawdust, and if my only choice is to fruit as-is.

Edited by MisterMuscaria (01/08/09 12:44 AM)

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Offlinedenger
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Re: Shiitake fruiting invitro [Re: MisterMuscaria]
    #9569315 - 01/08/09 06:37 AM (15 years, 2 months ago)

I guess what mycoharry is trying to say is that Shiitake are not grown in jars, you have to do bags. This jar you have can be used to inoculate a couple of bags.

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InvisibleMisterMuscaria
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Re: Shiitake fruiting invitro [Re: denger]
    #9570692 - 01/08/09 12:39 PM (15 years, 2 months ago)

Okay, thats what I was wanting to know.
Ill just fill the bags with brf and sawdust then.

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Offlinedenger
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Re: Shiitake fruiting invitro [Re: MisterMuscaria]
    #9571155 - 01/08/09 02:04 PM (15 years, 2 months ago)

Yes, and keep BRF down to no more then 15% by weight, Shiitake can form weird fruit when there is too much starch.

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OfflineMephistophelian
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Re: Shiitake fruiting invitro [Re: denger]
    #9571251 - 01/08/09 02:17 PM (15 years, 2 months ago)

So, Denger...your saying that you can replace bran for BRF in a pinch then...but avoid more then 15% because of the starch content.


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Offlinedenger
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Re: Shiitake fruiting invitro [Re: Mephistophelian]
    #9571575 - 01/08/09 03:15 PM (15 years, 2 months ago)

I am saying you can replace bran with almost any grain product milled medium-fine (including BRF, but its more $$ then WBS or bran), and with any grain product you should avoid putting in more then 15% (10% recommended) for shiitake's.

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InvisibleMisterMuscaria
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Re: Shiitake fruiting invitro [Re: denger]
    #9571588 - 01/08/09 03:18 PM (15 years, 2 months ago)

Would rye flour work?

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Offlinedenger
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Re: Shiitake fruiting invitro [Re: MisterMuscaria]
    #9571620 - 01/08/09 03:24 PM (15 years, 2 months ago)

Flour (unless its a whole grain flour) is not a good choice because it lacks the outer layers of the seeds, which contain most of the nitrogen. However, any whole grain product would work.

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Re: Shiitake fruiting invitro [Re: denger]
    #9571636 - 01/08/09 03:27 PM (15 years, 2 months ago)

So, then one must ask...is Bran necessarily the best nutrient additive then? I know Stamets always has mentioned Bran as the fundamental additive to sawdust blocks and eaters, but as you've mentioned medium grind grains suffice, but do they stand up to Bran?


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Offlinedenger
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Re: Shiitake fruiting invitro [Re: Mephistophelian]
    #9572880 - 01/08/09 07:04 PM (15 years, 2 months ago)

Quote:

Mephistophelian said:
So, then one must ask...is Bran necessarily the best nutrient additive then? I know Stamets always has mentioned Bran as the fundamental additive to sawdust blocks and eaters, but as you've mentioned medium grind grains suffice, but do they stand up to Bran?




That depends. GGMM has a huge table at the end with the analysis of various possible supplements. There are a number of things that seem fairly close. I have personally substituted bran with coarsely ground WBS with excellent results.

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InvisibleMisterMuscaria
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Re: Shiitake fruiting invitro [Re: denger]
    #9573257 - 01/08/09 07:58 PM (15 years, 2 months ago)

I think Ill go with the coarsely ground wbs! Thanks!

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OfflineMycoAu
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Re: Shiitake fruiting invitro [Re: MisterMuscaria]
    #9573338 - 01/08/09 08:10 PM (15 years, 2 months ago)



Yes, WBS "flour" (coarse grind) is fine for supplementing sawdust for use with L. edodes.  This bag is ~ 1 week out since inoculation with grain.

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Invisiblenoobieshroomie
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Re: Shiitake fruiting invitro [Re: MycoAu]
    #9573362 - 01/08/09 08:14 PM (15 years, 2 months ago)

i grew some lions mane that way
i could not get the damn cakes out of the jars


  -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineMycoAu
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Re: Shiitake fruiting invitro [Re: noobieshroomie]
    #9573392 - 01/08/09 08:17 PM (15 years, 2 months ago)

Those are nice fruits for a jar grow.  I'd recommend placing the jars on their side on top of a something to elevate them above the perlite next time.  This will allow a much more natural growth environment and will make it possible to harvest them much more easily (*fill the jars to the brim next time to get the fruits as close to the edge as possible). 

Or just use bags.

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Re: Shiitake fruiting invitro [Re: noobieshroomie]
    #9573449 - 01/08/09 08:24 PM (15 years, 2 months ago)

Quote:

noobieshroomie said:
i grew some lions mane that way
i could not get the damn cakes out of the jars


  -noobie-




Hericium grows pretty thick and sticky mycelium on the surface, no wonder you could not get them out. You'd get the same thing with normal sawdust+bran mixture: its the species not the mix that caused that.
Good grow, BTW. These are super tasty!

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Invisiblenoobieshroomie
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Re: Shiitake fruiting invitro [Re: denger]
    #9573522 - 01/08/09 08:32 PM (15 years, 2 months ago)

i plan on flipping the jars on their sides
just to see if they do better

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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