Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblepipelayer.rwr
Greenhorn

Registered: 12/23/08
Posts: 48
Loc: US
Liquid Culture Jars ???
    #9557596 - 01/06/09 12:58 PM (15 years, 2 months ago)

Ok I am just checking here, but i have been looking at the LC jars from mycopath.com. Do these just get injected and incubated just the same as the brf jars ??? I thought you needed to magnetically stir them. Also, will this work to make LC syringes to inoculate grain jars ???

Extras: Filter Print Post Top
Offlinedrumjam
Strangest
 User Gallery


Registered: 01/15/06
Posts: 248
Last seen: 8 years, 9 days
Re: Liquid Culture Jars ??? [Re: pipelayer.rwr]
    #9557787 - 01/06/09 01:39 PM (15 years, 2 months ago)

This is what I did out of Agar's posts.  The rubber ring makes it suitable for LC's.  Don't know if your looking to make or buy, but this was easy and all the suppliers are readable if you click the pics.
GL.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8742505/an/0/page/3

Extras: Filter Print Post Top
OfflineSpongiform
Some Cow
Male User Gallery

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Jars ??? [Re: drumjam]
    #9557822 - 01/06/09 01:45 PM (15 years, 2 months ago)

Using a stirbar of some sort will speed it up a little and help keep the mycelium broke up so it's easier to suck up in a syringe.

For mine, I just give them a swirl once in awhile but otherwise I leave them on a shelf at 70-75f alone.

Once they're ready you just suck up some of the mycelium into a syringe and use it to inoculate other stuff.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

Extras: Filter Print Post Top
OfflineMatler
Cosmic Trigger

Folding@home Statistics
Registered: 12/10/07
Posts: 32
Last seen: 8 years, 4 months
Re: Liquid Culture Jars ??? [Re: Spongiform]
    #9557948 - 01/06/09 02:13 PM (15 years, 2 months ago)

You do not need a stir bar. Simply inject your spores into the jar you bought from mycopath and away it will grow.

Extras: Filter Print Post Top
Offlinedrumjam
Strangest
 User Gallery


Registered: 01/15/06
Posts: 248
Last seen: 8 years, 9 days
Re: Liquid Culture Jars ??? [Re: Matler]
    #9558639 - 01/06/09 04:29 PM (15 years, 2 months ago)

I built a stir plate and it does speed it up a little, but what I found is that it keeps the growth broken up and more parts would grow, the speed part, but it was convenient to suck up and spread. Not needed just convenient.

Extras: Filter Print Post Top
OfflineSpongiform
Some Cow
Male User Gallery

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Jars ??? [Re: drumjam]
    #9558728 - 01/06/09 04:42 PM (15 years, 2 months ago)

I have to suck up some water/air and blow it back into the mass of mycelium to break it up.  I just go ahead and do it a few times before I try to suck anything up to use.  Adds about a minute to my inoculation times.  No big deal really.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

Extras: Filter Print Post Top
Offlineronjohn7779
Stranger


Registered: 10/28/08
Posts: 1,373
Last seen: 12 years, 9 months
Re: Liquid Culture Jars ??? [Re: Spongiform]
    #9558843 - 01/06/09 04:59 PM (15 years, 2 months ago)

Dude that's kind of a rip off they're charging 11 bucks for a Jar that has corn syrup and water in it (and only 4% of it is corn syrup). You could do all of that and more for less than 10 bucks and make way more LC's. If you're going to sensually blow 11 bucks you might as well get the jars with the malt extract as I've heard it works way better.

Also a self healing port can be easily made with a 4 dollar tub of a epoxy they sell nearly everywhere.

On a side note I wouldn't trust a jar that wasn't pressure cooked same day with in a couple of hours. LC tend to contaminate real easy. Of my last batch of six only 1 jar made it. I also have a real liberal use of gloves, needles, and syringes (I get them all free). So they were all clean.


--------------------
"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

Edited by ronjohn7779 (01/06/09 05:01 PM)

Extras: Filter Print Post Top
OfflineSpongiform
Some Cow
Male User Gallery

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Jars ??? [Re: ronjohn7779]
    #9558955 - 01/06/09 05:17 PM (15 years, 2 months ago)

It's really not a bad deal for someone who just wants 1 jar of the stuff.

Remember - these businesses have to federal/state and maybe even county/local taxes.  They gotta pay for business licenses, federal employer licenses, and all sorts of associated fees/taxes (like workmen's comp).

Also they have to pay for your website, a merchant account to accept credit cards.  They're also paying for advertising space on google or similar and don't forget that they're paying a minimum of $200 /month to be a shoomery sponsor.

So they're probably paying about $2 in materials/cost to make these then the rest is markup for profit/taxes/fees - they gotta turn a profit :-D

All things considered, $10 is not a bad deal.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

Extras: Filter Print Post Top
Offlinemudkip
I heard U Like Me!
Male

Registered: 11/08/08
Posts: 428
Last seen: 10 years, 3 months
Re: Liquid Culture Jars ??? [Re: Spongiform]
    #9559038 - 01/06/09 05:29 PM (15 years, 2 months ago)

Is it really 200 a month to be a sponsor? Where did you get that information?


--------------------


Extras: Filter Print Post Top
Offlinexbrutalx233
Learner
Male


Registered: 09/10/08
Posts: 1,001
Loc: Planet X
Last seen: 10 years, 7 months
Re: Liquid Culture Jars ??? [Re: mudkip]
    #9559198 - 01/06/09 05:57 PM (15 years, 2 months ago)

Not to mention 4% corn syrup is ideal...


--------------------
:mushroom2:

:mushroom2:


I do not grow anything that is illegal... Everything is of the legal variety... if someone mentions any illegal activity it isn't me...

Extras: Filter Print Post Top
InvisibleShroominit
Part Time Mycologist


Folding@home Statistics
Registered: 11/29/08
Posts: 4,662
Re: Liquid Culture Jars ??? [Re: xbrutalx233]
    #9559233 - 01/06/09 06:02 PM (15 years, 2 months ago)

The shroomery store says 200/mo to be a sponsor.

And yes, $1+ for the jar, $3-4 for the corn syrup, $3 for the distilled water, and the time to weigh, prep and sterilize (probably autoclave and UV).

Also, you know it's likely not to be contam'd. Probably worth the $11 to people starting out.

Extras: Filter Print Post Top
Offlineronjohn7779
Stranger


Registered: 10/28/08
Posts: 1,373
Last seen: 12 years, 9 months
Re: Liquid Culture Jars ??? [Re: xbrutalx233]
    #9559237 - 01/06/09 06:03 PM (15 years, 2 months ago)

Quote:

xbrutalx233 said:
Not to mention 4% corn syrup is ideal...



Yeah that's why I mentioned that. The site doesn't list percentages. I just assumed they used that much.


--------------------
"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

Extras: Filter Print Post Top
Offlinexbrutalx233
Learner
Male


Registered: 09/10/08
Posts: 1,001
Loc: Planet X
Last seen: 10 years, 7 months
Re: Liquid Culture Jars ??? [Re: ronjohn7779]
    #9559346 - 01/06/09 06:18 PM (15 years, 2 months ago)

Oh I thought you were saying something was wrong with 4%


--------------------
:mushroom2:

:mushroom2:


I do not grow anything that is illegal... Everything is of the legal variety... if someone mentions any illegal activity it isn't me...

Extras: Filter Print Post Top
OfflineSpongiform
Some Cow
Male User Gallery

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Jars ??? [Re: xbrutalx233]
    #9559557 - 01/06/09 06:54 PM (15 years, 2 months ago)

A whole bottle of karo syrup runs like $2 and you need like 1 or 2 tablespoons to reach 4% in a pint jar.

That being said they probably end up making like $2-3 profit off it.

When I did my first batch of Lme/Dex LC's I was concerned about the sediment, so I inoculate 1 and left 3 to sit around as control jars.  After about a month the control jars were still good.

As long as it was properly sterilized and the filters are made correctly it should be good for a long, long time.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

Extras: Filter Print Post Top
OfflineiMtRiPpIn
Lookin' fer Luv
 User Gallery


Registered: 06/04/08
Posts: 530
Last seen: 11 years, 5 months
Re: Liquid Culture Jars ??? [Re: pipelayer.rwr]
    #9559655 - 01/06/09 07:06 PM (15 years, 2 months ago)

Quote:

pipelayer.rwr said:
Ok I am just checking here, but i have been looking at the LC jars from mycopath.com. Do these just get injected and incubated just the same as the brf jars ??? I thought you needed to magnetically stir them. Also, will this work to make LC syringes to inoculate grain jars ???




Inject 2cc's of spore solution and incubate at room temp (75F).  You do not need to "magnetically" stir them.  You can stir them a couple times a day by hand by swirling the solution around in the bottle.  Be careful not to get the filter wet, it can draw in contamination if wet.  Yes, it will work to make LC syringes to inoculate grain jars.


--------------------

Extras: Filter Print Post Top
OfflineSpongiform
Some Cow
Male User Gallery

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Jars ??? [Re: iMtRiPpIn]
    #9559704 - 01/06/09 07:14 PM (15 years, 2 months ago)

2cc's is a bit much really.  I wouldn't use more then 1/2cc.  Last one I made I used 3 drops.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

Extras: Filter Print Post Top
Offlineronjohn7779
Stranger


Registered: 10/28/08
Posts: 1,373
Last seen: 12 years, 9 months
Re: Liquid Culture Jars ??? [Re: Spongiform]
    #9559840 - 01/06/09 07:31 PM (15 years, 2 months ago)

Quote:

Spongiform said:
2cc's is a bit much really.  I wouldn't use more then 1/2cc.  Last one I made I used 3 drops.



I agree. In all of the jars I used 1 to 2 ccs became contaminated (I've never had contams before this). It makes sense. Spores are dirty and the more dirty spores you have in your jars the more contams you may get. The one I used a 1/2 cc came out perfect. Even a couple of drops from a viable syringe will have thousands of spores in it.

When I first asked about making an LC someone here told me to only use a few drops. I dismissed this advise, but in reality they might have been right.


--------------------
"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

Extras: Filter Print Post Top
Offlinehunter4321
Cultivator (Training)
 User Gallery


Registered: 10/13/08
Posts: 236
Last seen: 14 years, 3 months
Re: Liquid Culture Jars ??? [Re: ronjohn7779]
    #9559859 - 01/06/09 07:35 PM (15 years, 2 months ago)

buy you a pressure cooker, if you don't own one. AND READ!!


--------------------


I have Golden Teacher prints for trade, 2nd generation of a very fast and dense strain,and PES Amazon prints, PM me if you are interested.

Extras: Filter Print Post Top
Offlinehunter4321
Cultivator (Training)
 User Gallery


Registered: 10/13/08
Posts: 236
Last seen: 14 years, 3 months
Re: Liquid Culture Jars ??? [Re: hunter4321]
    #9559883 - 01/06/09 07:38 PM (15 years, 2 months ago)

I prefer a cloned LC, or a true isolate from agar wedge, for my LC's


--------------------


I have Golden Teacher prints for trade, 2nd generation of a very fast and dense strain,and PES Amazon prints, PM me if you are interested.

Extras: Filter Print Post Top
Invisiblepipelayer.rwr
Greenhorn

Registered: 12/23/08
Posts: 48
Loc: US
Re: Liquid Culture Jars ??? [Re: hunter4321]
    #9562651 - 01/07/09 05:39 AM (15 years, 2 months ago)

Thank you for the great Information ?

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 14,445 10 02/28/06 08:35 PM
by Limelight_Liver
* liquid culture failure gray1 6,001 3 07/19/01 09:37 PM
by Azure
* Starting a simple liquid culture method Garret_Hill 9,183 3 03/11/02 09:49 AM
by YourHighness
* liquid culture using h2o2 baja 9,115 14 03/09/17 10:00 PM
by 3B3B4E
* liquid culture pr? socratesmind 2,034 8 01/18/03 06:45 PM
by ISH
* need input, liquid culture! tripyfungi 1,323 1 11/19/01 07:43 PM
by razer
* liquid culture Anonymous 599 1 09/24/02 03:54 PM
by mickey_rourke
* Brown Rice Syrup SixTango 1,585 3 07/20/02 07:31 PM
by SixTango

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
948 topic views. 32 members, 163 guests and 146 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.011 seconds on 15 queries.