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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Mushroom clonning... * 1
    #949722 -

Hello !
I have tried to clone an oyster mushroom (from the grocery store). I have 6 agar  jars (instead of petri dishes) with 6 little pieces of mushroom. This is the 4th day now, and the little pieces of mushroom have "furr" on them... Is it normal ?! :confused: 

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #949904 -

Im no pro,
But If its white and fluffy its probably myceiliem and yes its a good thing.
:smirk: 


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Listen, or your tongue will keep you deaf. :wink:
ŦēҜй? - ??ĜįĈ?? ҒűČҝĮńĜ ?đVǻŃčЄмЄńŦ

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #950112 -

It could well be mycellium....or it could infact me a contam. Welcome to the fun :wink:

First off, what oyster strain are you trying to clone? Did you use aseptic conditions?

I'll dig out the mycellium characteristics for whatever oyster strain and post them for you.

-psyco


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It may look like a button mushroom right now, but wait until you see how it grows!

Edited by psyconaut (10/10/02 04:13 PM)

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Re: Mushroom clonning... [Re: psyconaut] * 1
    #954652 -

I don't know what strain it is :crazy:  All I know is that I've bought a pleurotus ostreatus...
(Well, I think so)

This is what it looks like ( the white fur and there is also a contam there) Do you think it might be mycelium ? All my 6 jars have that. I've used a glove box, but this is my first time... :confused:


 

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #954664 -

Isolate the white mycelium from the contamination ASAP.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #956594 -

It looks good! The white stuff in the center is mycellium. Just cut a piece that is as far as possible from contamn and transfer it to a clean jar.

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #957145 -

It looks like nice rhizomorphic growth, although the pic is a little blury. Make sure to steralize your instruments (scalpel, whatever) before doing the surgery to remove the contam!

Keep going....don't let a little bit of contamination put you off :smile:

-psyco


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It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Mushroom clonning... [Re: psyconaut] * 1
    #957167 -

>before doing the surgery to remove the contam!

The golden Stametsian rule: You should allways remove the healthy growth from contamination, never the other way round.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Mushroom clonning... [Re: Anno] * 1
    #958900 -

Anno is right. You have a far more greatter risk of recontaming the culture if you try to remove the contam, due to you might spread more contam spores all over the culture, or by not getting it all. So it is much easier to just take a good part of the contamed cuture and put it on a new sterile agar dish. If it still contams well best to start over. Good luck................peace


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GSAfarmer Joshua

"Don't live someone elses dream."..........GSAfarmer

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #966894 -

Hey guys ! I followed your advices and everything seems fine !
I have another question... When do I know that the agar has been completly colonized ? When the mycelium will cover all the agar ?

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #967644 -

Correct. Or, if you're not so lucky, it'll go invitro on you ;-)

-psyco


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It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #967666 -

mycellium will only colonize the top surface of the agar.
dont expect it to colonize the sides and/or bottom of the jars.

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Re: Mushroom clonning... [Re: solidstate] * 1
    #968468 -

...and furthermore,the usual advice is to transfer it before it even reaches the sides of the dish (or jar)....

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Re: Mushroom clonning... [Re: solidstate] * 1
    #969270 -

Jars? I was assuming he was doing this on a petri dish!  :shocked:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Mushroom clonning... [Re: psyconaut] * 1
    #970208 -

Jars... hehehe I messed up with the petri dishes; I just don't like them. Anyways, I believe I have a fully colonised jar now :grin: and I've never been happier in my life!  :smirk: 

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #970429 -

Good going! We'll be expecting pics as you progress :wink:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #983570 -

glad to hear things are fine now. keep us posted. and good luck.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.

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Re: Mushroom clonning... [Re: canid] * 1
    #984061 -


Well, I have extented my cultures to almost 20 jars now. I've also made a incubator, so I've put my cultures there and they are growing faster!
The culture in the pic is still growing, even if I had to cut some parts of it !! The mycelium is growing vertically!!!  :ooo:
I have two jars of grain right now, and I'll inoculate them tomorrow! 

Edited by MushMushi (10/22/02 05:37 PM)

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #995093 -

I think I have some problems with my cultures... the agar is turning to a dark yellow and mycelium stops growing... why is that so???

By the way, I have an incubator and the temperature inside is +/- 75
Could the heat be a problem ??
:mad: 

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #1017945 -

Hey!
I've inoculate some rye jars with the mycelium and this is what I've got... Is it normal??



It is the same jar in every pic... It that mycelium ? It's been almost a week now since I inoculated them... :confused: 

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Re: Mushroom clonning... [Re: MushMushi] * 1
    #1033689 -

It's normal for mycelium from this strange to turn yellow or orange.

Your pics aren't too clear...is it now forming a woven mat?

-psy


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!

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