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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Mushroom clonning...
    #949722 - 10/10/02 03:16 PM (14 years, 9 months ago)

Hello !
I have tried to clone an oyster mushroom (from the grocery store). I have 6 agar  jars (instead of petri dishes) with 6 little pieces of mushroom. This is the 4th day now, and the little pieces of mushroom have "furr" on them... Is it normal ?! :confused: 


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OfflineTeKn0
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Re: Mushroom clonning... [Re: MushMushi]
    #949904 - 10/10/02 04:51 PM (14 years, 9 months ago)

Im no pro,
But If its white and fluffy its probably myceiliem and yes its a good thing.
:smirk: 


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Invisiblepsyconaut
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Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Mushroom clonning... [Re: MushMushi]
    #950112 - 10/10/02 06:10 PM (14 years, 9 months ago)

It could well be mycellium....or it could infact me a contam. Welcome to the fun :wink:

First off, what oyster strain are you trying to clone? Did you use aseptic conditions?

I'll dig out the mycellium characteristics for whatever oyster strain and post them for you.

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


Edited by psyconaut (10/10/02 06:13 PM)


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: psyconaut]
    #954652 - 10/12/02 08:18 AM (14 years, 9 months ago)

I don't know what strain it is :crazy:  All I know is that I've bought a pleurotus ostreatus...
(Well, I think so)

This is what it looks like ( the white fur and there is also a contam there) Do you think it might be mycelium ? All my 6 jars have that. I've used a glove box, but this is my first time... :confused:


 


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OfflineAnnoA
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Re: Mushroom clonning... [Re: MushMushi]
    #954664 - 10/12/02 08:41 AM (14 years, 9 months ago)

Isolate the white mycelium from the contamination ASAP.


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Offlinezeronio
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Re: Mushroom clonning... [Re: MushMushi]
    #956594 - 10/13/02 10:22 AM (14 years, 9 months ago)

It looks good! The white stuff in the center is mycellium. Just cut a piece that is as far as possible from contamn and transfer it to a clean jar.


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Invisiblepsyconaut
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Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Mushroom clonning... [Re: MushMushi]
    #957145 - 10/13/02 04:22 PM (14 years, 9 months ago)

It looks like nice rhizomorphic growth, although the pic is a little blury. Make sure to steralize your instruments (scalpel, whatever) before doing the surgery to remove the contam!

Keep going....don't let a little bit of contamination put you off :smile:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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OfflineAnnoA
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Re: Mushroom clonning... [Re: psyconaut]
    #957167 - 10/13/02 04:32 PM (14 years, 9 months ago)

>before doing the surgery to remove the contam!

The golden Stametsian rule: You should allways remove the healthy growth from contamination, never the other way round.


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OfflineGSAfarmer
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Registered: 06/21/02
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Re: Mushroom clonning... [Re: Anno]
    #958900 - 10/14/02 04:04 AM (14 years, 9 months ago)

Anno is right. You have a far more greatter risk of recontaming the culture if you try to remove the contam, due to you might spread more contam spores all over the culture, or by not getting it all. So it is much easier to just take a good part of the contamed cuture and put it on a new sterile agar dish. If it still contams well best to start over. Good luck................peace


--------------------

GSAfarmer Joshua

"Don't live someone elses dream."..........GSAfarmer


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: MushMushi]
    #966894 - 10/16/02 08:25 PM (14 years, 9 months ago)

Hey guys ! I followed your advices and everything seems fine !
I have another question... When do I know that the agar has been completly colonized ? When the mycelium will cover all the agar ?


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Invisiblepsyconaut
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Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Mushroom clonning... [Re: MushMushi]
    #967644 - 10/17/02 01:13 AM (14 years, 9 months ago)

Correct. Or, if you're not so lucky, it'll go invitro on you ;-)

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Invisiblesolidstate
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Registered: 04/09/00
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Re: Mushroom clonning... [Re: MushMushi]
    #967666 - 10/17/02 01:23 AM (14 years, 9 months ago)

mycellium will only colonize the top surface of the agar.
dont expect it to colonize the sides and/or bottom of the jars.


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Offlineaural
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Registered: 05/04/00
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Re: Mushroom clonning... [Re: solidstate]
    #968468 - 10/17/02 10:51 AM (14 years, 9 months ago)

...and furthermore,the usual advice is to transfer it before it even reaches the sides of the dish (or jar)....


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Invisiblepsyconaut
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Registered: 05/22/02
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Re: Mushroom clonning... [Re: solidstate]
    #969270 - 10/17/02 03:31 PM (14 years, 9 months ago)

Jars? I was assuming he was doing this on a petri dish!  :shocked:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: psyconaut]
    #970208 - 10/17/02 08:03 PM (14 years, 9 months ago)

Jars... hehehe I messed up with the petri dishes; I just don't like them. Anyways, I believe I have a fully colonised jar now :grin: and I've never been happier in my life!  :smirk: 


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Invisiblepsyconaut
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Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Mushroom clonning... [Re: MushMushi]
    #970429 - 10/17/02 09:48 PM (14 years, 9 months ago)

Good going! We'll be expecting pics as you progress :wink:

-psyco


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Offlinecanid
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Re: Mushroom clonning... [Re: MushMushi]
    #983570 - 10/22/02 05:33 PM (14 years, 9 months ago)

glad to hear things are fine now. keep us posted. and good luck.


--------------------



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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: canid]
    #984061 - 10/22/02 07:35 PM (14 years, 9 months ago)


Well, I have extented my cultures to almost 20 jars now. I've also made a incubator, so I've put my cultures there and they are growing faster!
The culture in the pic is still growing, even if I had to cut some parts of it !! The mycelium is growing vertically!!!  :ooo:
I have two jars of grain right now, and I'll inoculate them tomorrow! 


Edited by MushMushi (10/22/02 07:37 PM)


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: MushMushi]
    #995093 - 10/26/02 06:46 PM (14 years, 8 months ago)

I think I have some problems with my cultures... the agar is turning to a dark yellow and mycelium stops growing... why is that so???

By the way, I have an incubator and the temperature inside is +/- 75
Could the heat be a problem ??
:mad: 


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Mushroom clonning... [Re: MushMushi]
    #1017945 - 11/03/02 10:37 AM (14 years, 8 months ago)

Hey!
I've inoculate some rye jars with the mycelium and this is what I've got... Is it normal??



It is the same jar in every pic... It that mycelium ? It's been almost a week now since I inoculated them... :confused: 


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