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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: antibiotic agar [Re: THEDANGLER]
#4044367 - 04/12/05 04:28 AM (18 years, 10 months ago) |
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Quote:
THEDANGLER said: their are spores out their that can survive pressure cooking im sure theirs a couple species that can survive h202
this is not corrects, its certain ENDOspores on/in grains that can survive the pc. peace ohm
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MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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THEDANGLER
The Mushroom Tip


Registered: 11/01/04
Posts: 312
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Re: antibiotic agar [Re: ohmatic]
#4044771 - 04/12/05 08:24 AM (18 years, 10 months ago) |
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their are bacteira found in the marrianas (sp) trench that are under extreme pressure on little volcanos donw their...shit a fucking pc would be to cold for them..
-------------------- TONS OF SPORES TO TRADE PM ME!!!!
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THEDANGLER
The Mushroom Tip


Registered: 11/01/04
Posts: 312
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Re: antibiotic agar [Re: THEDANGLER]
#4044787 - 04/12/05 08:31 AM (18 years, 10 months ago) |
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check it...http://www.marinetech.org/nine_degrees/index.php
403degrees on the ocean floor...aka more then you 15psi
-------------------- TONS OF SPORES TO TRADE PM ME!!!!
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THEDANGLER
The Mushroom Tip


Registered: 11/01/04
Posts: 312
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Re: antibiotic agar [Re: THEDANGLER]
#4044797 - 04/12/05 08:34 AM (18 years, 10 months ago) |
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thats not really what i said in the original post..about spores...i ment to say all contams..bacteria mold..what ever...im just saying bacteria evolve so fast their IS some bacteria or mold out their were spore or what ever will survive a diluted peroxide solution..
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Mindzpore
Psychedelicious


Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 7 years, 4 months
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Re: antibiotic agar [Re: THEDANGLER] 1
#4044995 - 04/12/05 09:54 AM (18 years, 10 months ago) |
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Quote:
THEDANGLER said: yeah but what if their is a couple spores that arnt destroyed...aka they evolve....nothing is impossible..
evolution does not work like that.
methods of sterilization as they are today are usually far more powerful than they actually need to be to just kill the contaminants. and why? to prevent the kind of evolution that your talking about.
the specifics of the organisms are set from their conception, and are NOT going to suddenly start to evolve/change in the pc (or h2o2). how evolution works is that if there is one bacteria/spore that is just a little bit more hardy than the others AND you do a botch sterilization... and that spore/bacteria survive. then its descendants/clones will share that "slightly more hardy" trait. and maby one out of a million of them again have a slight increase of hardiness.
so the type of evolution of pathogens and contaminants that you seem to refer to are the result of human idiocy, misuse of antibiotics, sloppy sterilization etc. do your job right, and there is no "bad" evolution.
-------------------- Mindzpores words of wisdom: "If you think something is foolproof, you just haven't met proper fools". Wiccan_Seeker said: "It is better to adjust to become a better listener than to keep on cranking up the volume".
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THEDANGLER
The Mushroom Tip


Registered: 11/01/04
Posts: 312
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Re: antibiotic agar [Re: Mindzpore]
#4046199 - 04/12/05 01:59 PM (18 years, 10 months ago) |
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yeah I'm talking theoretically over time..hows does peroxide kill spores? yes you are right about evolution, I'm just saying their are organisms that can survive pressure cooking and i think their has to be some spore out their some were that can survive a little peroxide...please tell me how peroxide kills spores if your so smart..
-------------------- TONS OF SPORES TO TRADE PM ME!!!!
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Mindzpore
Psychedelicious


Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 7 years, 4 months
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Re: antibiotic agar [Re: THEDANGLER]
#4049167 - 04/13/05 05:16 AM (18 years, 10 months ago) |
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h2o2 is a water molecule with an extra oxygen. this is not a stable compound, and the extra oxygen is very eager to get away and reacts to almost anything it can...
now I'm not entirely sure, but unless my memory fails me. the way h2o2 kills single cell entities is by destroying its cell membrane (by hyperoxidisation of course).
-------------------- Mindzpores words of wisdom: "If you think something is foolproof, you just haven't met proper fools". Wiccan_Seeker said: "It is better to adjust to become a better listener than to keep on cranking up the volume".
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rungi
journeymana

Registered: 07/11/01
Posts: 212
Last seen: 15 years, 1 month
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Re: antibiotic agar [Re: ohmatic]
#9489468 - 12/24/08 01:55 PM (15 years, 1 month ago) |
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Will the hot temperature kill or mutate the mycelium?
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shroomophile
ShroomitusFidelis



Registered: 08/20/02
Posts: 762
Loc: USA
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Quote:
THEDANGLER said: yah well what if your peroxide perduces a resistant strain...ever think of that? the plates were poured in a glove box..
A oxidizer is a oxidizer.However,as we see with bleach,a few spores,germs,virus can survive.It makes it hard if 998,343 spores dies out of a hundred thousand.Not much to breed with.Peroxidated agar is best left to cultures.Gentamyicen sulfate will go thru the autoclave for the antibiotic end.
-------------------- Once the mighty oak,was a nut who held his ground.
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