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 Omnicracker
Crusted Trolltivator
Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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 Re: Liquid Culture Comparison Project  [Re: Damion5050]
#9515642 - 12/29/08 11:45 PM (15 years, 3 months ago) |
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LCs are sexy. like what a multidimensional tryptamine-being would release at orgasm.
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 mycofanatic
 
Registered: 06/12/08
Posts: 322
Last seen: 10 years, 1 month
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Re: Liquid Culture Comparison Project [Re: Damion5050]
#9515750 - 12/30/08 12:04 AM (15 years, 3 months ago) |
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This was a very nice side by side comparison. I think I'm going to give the malt extract and dextrose combo a try tomorrow, thanks for the info...
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: mycofanatic]
#9538301 - 01/03/09 03:08 PM (15 years, 3 months ago) |
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It's been about 10 days now.
The Lme/Dex jar is over 90% colonized.
The Sugar in the RAW is around 50%
Confectionary is about 20-25%
The Karo and Table Sugar's are around 10-15% and have not seemed to grow much in the last few days.
So based on my initial finding I would suggest using Sugar in the Raw over karo. It's about the same price and easier to measure and works much better.
If you can afford to order the Lme/Dex, DO IT! :-D
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9538520 - 01/03/09 03:39 PM (15 years, 3 months ago) |
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Quote:
Spongiform said:
It's been about 10 days now.
The Lme/Dex jar is over 90% colonized.
The Sugar in the RAW is around 50%
Confectionary is about 20-25%
Weird, and how long have I been preaching about using light malt powder?
Reminds me of a thread I started the other day where I was wondering what people were using
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/9475877/an/0/page/0
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: tahoe]
#9538823 - 01/03/09 04:24 PM (15 years, 3 months ago) |
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The results speak for themselves :-D
Maybe I should try it again using a 2% sugar solution. Could be OD'ing on the sugar.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9538913 - 01/03/09 04:38 PM (15 years, 3 months ago) |
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just use straight malt. .5-2 grams per 500ml. It isnt rocket science
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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987
Teh=The
I need to proofread
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Liquid Culture Comparison Project [Re: tahoe]
#9539584 - 01/03/09 06:48 PM (15 years, 3 months ago) |
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Cool. Thanks for the update!
If you don't mind me adding, I didn't notice a difference in my LC's (stirred and unstirred). They were both shaken after PCing and had no GE.
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: tahoe]
#9539591 - 01/03/09 06:50 PM (15 years, 3 months ago) |
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Quote:
tahoe said:
just use straight malt. .5-2 grams per 500ml. It isnt rocket science
I meant, I might try the other sugars again but less.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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archivist
5-HT
Registered: 06/06/07
Posts: 1,010
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9541741 - 01/04/09 02:02 AM (15 years, 3 months ago) |
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Quote:
Spongiform said:
It's been about 10 days now.
The Lme/Dex jar is over 90% colonized.
The Sugar in the RAW is around 50%
Confectionary is about 20-25%
The Karo and Table Sugar's are around 10-15% and have not seemed to grow much in the last few days.
By % colonized do you mean the approximate "volume" of the mycelium at rest in proportion to the total liquid? Some updated pictures would be neat 
90% sounds thick! I have a Karo one going on 10 days as well and it's probably 15% and doesn't look like it will go much further. I'd like to try some turbinado next and will probably attempt some lme/dex when I buy some malt for an agar recipe.
Have you tried inoculating some test jars with these to see how they perform on a grain medium? (and also to make sure none of that growth is from contams?)
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Proud supporter of the canning jar industry.
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: archivist]
#9543151 - 01/04/09 11:07 AM (15 years, 3 months ago) |
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I have not tested these yet but I will use them. They all look pretty normal except the powdered confectionery sugar LC. It's pretty cloudy.
It was cloudy right after PC'ing though, so I attributed as a byproduct of the sugar.
The %'s are approximate volumes. The Karo and table sugar have lots of little puff balls of mycelium. Sometimes they'll settle and appear as one large ball but after swirling they come apart again.
The lme/dex is just filled up damn near to the top. Big masses/chunks of mycelium that all form into a big blob. The last LC I made like this I had to spend a couple minutes sucking in air/water and blowing it back into the masses to break them up so I could draw them out with my 18g needle.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9581266 - 01/10/09 02:56 AM (15 years, 3 months ago) |
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Just a little update. The lme/dex jar is almost one giant blob now and has actually started to colonize the surface with nice white mycelium. I thought it was contaminated for a sec.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9591478 - 01/11/09 08:01 PM (15 years, 3 months ago) |
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Ok, here's the final results. It's now Day 18.
Jars #2 through 5 have not shown any growth in over a week.
Jar #1 (lme/dex) has continued to grow. Not only colonizing virtually the entire liquid but also the surface and then attempting to grow up the side of the jar too.
Jar #1 - LME / Dex
Jar #2 - Sugar in the Raw
Jar #3 - Powdered Confectionery Sugar (love this shiat in my coffee now)
Jar #4 - Karo
Jar #5 - Table Sugar
The clear winner here is lme/dex but the RAW is a close close second.
Not as much growth but still it did very well and it's also much cheaper and easier to do for newbs. It's cheap, easy to find and already premeasured so it's pretty hard to screw that up.
I'll be re-writing this up in a single post in my journal.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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xswaveyx
SwaveY
Registered: 08/22/08
Posts: 1,077
Loc: East
Last seen: 3 years, 2 months
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9591582 - 01/11/09 08:24 PM (15 years, 3 months ago) |
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very interesting. Why didnt you do honey? Call me a newb but i dont know what lme/dex is... dextrose?
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Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.
My Growlog with Redboy,Amazon,and Brazilian
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: Liquid Culture Comparison Project [Re: xswaveyx]
#9591594 - 01/11/09 08:26 PM (15 years, 3 months ago) |
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dextrose is also known as corn sugar.
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! 
Last seen: 3 years, 2 months
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Re: Liquid Culture Comparison Project [Re: Liquidkick]
#9591600 - 01/11/09 08:28 PM (15 years, 3 months ago) |
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LME and DEX Is Light Malt Extract and Dextrose/Sugar
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freetree77
king louie
Registered: 12/09/06
Posts: 265
Loc: all across the universe
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Re: Liquid Culture Comparison Project [Re: xswaveyx]
#9591626 - 01/11/09 08:34 PM (15 years, 3 months ago) |
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cool experiment. last time i had mycelium grow on the surface and sides of the jar, it soon turned green. but that was only after i had already used some for a couple jars, so i may have introduced the contaminant myself. then again, all 3 jars contaminated. could have been because the wbs was too wet or because of the lc. hope you have better luck.
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Sinister Minister
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xswaveyx
SwaveY
Registered: 08/22/08
Posts: 1,077
Loc: East
Last seen: 3 years, 2 months
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Re: Liquid Culture Comparison Project [Re: Damion5050]
#9591635 - 01/11/09 08:35 PM (15 years, 3 months ago) |
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i thought karo was corn sugar? theres obviously a difference that im missing.
i used honey when i tried to make an LC...and failed. But i saw plenty of honey teks on here that worked?i dunno.
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Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.
My Growlog with Redboy,Amazon,and Brazilian
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 7 months
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Re: Liquid Culture Comparison Project [Re: xswaveyx]
#9591828 - 01/11/09 09:15 PM (15 years, 3 months ago) |
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I didn't have any honey and forgot to buy some is all.
I believe the reason it grew on the surface is because the mycelial mass grew up to the surface. I had it happen in 3 other lme/dex jars too. I used up almost that whole jar of lme/dex tonight.
https://files.shroomery.org/files/09-002/172933981-PE---Start-Shelf.jpg
And filled up a syringe for later use.
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Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9591889 - 01/11/09 09:26 PM (15 years, 3 months ago) |
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Awesome experiment spongiform. I love it, tomorrow I will buy LME/Dex to add to my arsenal.
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freetree77
king louie
Registered: 12/09/06
Posts: 265
Loc: all across the universe
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Re: Liquid Culture Comparison Project [Re: Spongiform]
#9591890 - 01/11/09 09:26 PM (15 years, 3 months ago) |
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whoa you do your work in tupperware? how does that work out? do you pressure cook those? well right on
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Sinister Minister
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