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Invisibleslacker008
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Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Pre-Poured Pitri?
    #9447994 - 12/17/08 02:57 AM (13 years, 5 months ago)

I know this sounds silly but is there any place that sells sterile pitri dishes with agar already in them? Maybe I miffed something up but I cant seem to get the agar to NOT contaminate.


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..beneath the chaos of the world, all is secretly well..

Rebuilding my DNA.


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Invisiblenoobieshroomie
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Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Pre-Poured Pitri? [Re: slacker008]
    #9447997 - 12/17/08 02:58 AM (13 years, 5 months ago)

ill hook you up with some

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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Invisibletripchip
The Mushroom Monkey
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Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448010 - 12/17/08 03:01 AM (13 years, 5 months ago)

wow noobie i love that pic of you with the shrooms.. man i wish i had a clone of that shit!


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Invisibleslacker008
Resident Psychiatrist
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Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448016 - 12/17/08 03:02 AM (13 years, 5 months ago)

That'd be a lifesaver. Im wanting to do some cloning work but its hard to do when I cant get agar pouring down.


--------------------
..beneath the chaos of the world, all is secretly well..

Rebuilding my DNA.


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Invisiblenoobieshroomie
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Posts: 12,769
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Re: Pre-Poured Pitri? [Re: slacker008]
    #9448028 - 12/17/08 03:04 AM (13 years, 5 months ago)

thanks trip
slacker i can send you some pre poured petries

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
Invisibletripchip
The Mushroom Monkey
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Folding@home Statistics
Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: Pre-Poured Pitri? [Re: slacker008]
    #9448033 - 12/17/08 03:05 AM (13 years, 5 months ago)

maybe you should talk about your method of making it. I have a dish that is about 4 months old never used and it has 0 contams. im waiting to see how long it can stay contam free for.


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Invisibletripchip
The Mushroom Monkey
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Folding@home Statistics
Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: Pre-Poured Pitri? [Re: tripchip]
    #9448042 - 12/17/08 03:07 AM (13 years, 5 months ago)

send me some too newbie, just dont accidently drop a piece of tissue on it :grin:


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Psilocybe Cubensis

Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba.

                                    :mushroom2:CHIP:mushroom2:


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Invisiblenoobieshroomie
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Re: Pre-Poured Pitri? [Re: tripchip]
    #9448057 - 12/17/08 03:14 AM (13 years, 5 months ago)

trip
i can send you a few blanks
or one with a lions mane culture
your choice

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Edited by noobieshroomie (12/17/08 03:17 AM)


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Invisibletripchip
The Mushroom Monkey
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Folding@home Statistics
Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448065 - 12/17/08 03:17 AM (13 years, 5 months ago)

nah im good. unless lions mane is code word..JK ill discover my own fatties that will put yours to shame!:evil:


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Invisiblenoobieshroomie
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Posts: 12,769
Loc: Not Too Sure Flag
Re: Pre-Poured Pitri? [Re: tripchip]
    #9448075 - 12/17/08 03:20 AM (13 years, 5 months ago)

Quote:

tripchip said:
nah im good. unless lions mane is code word..JK ill discover my own fatties that will put yours to shame!:evil:



ha ha ha
lions mane is a choice edible
it tastes like lobster when cooked right:)
PM me

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
Invisibletripchip
The Mushroom Monkey
Female User Gallery


Folding@home Statistics
Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448093 - 12/17/08 03:24 AM (13 years, 5 months ago)

LOL its all good dude. im cool. thanks anyways. your a good guy :thumbup:


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InvisibleShroominit
Part Time Mycologist


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Registered: 11/29/08
Posts: 4,662
Re: Pre-Poured Pitri? [Re: tripchip]
    #9448106 - 12/17/08 03:29 AM (13 years, 5 months ago)

Noobie, you're awesome. (so is that pic haha)

Slacker, petri dishes 'breathe' out the sides, so you want to keep them in a sterile area after making them. Also, you want to pour in a glove box or even better a flow hood to keep the contams out.


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Invisibleslacker008
Resident Psychiatrist
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Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Re: Pre-Poured Pitri? [Re: Shroominit]
    #9448110 - 12/17/08 03:31 AM (13 years, 5 months ago)

I dont have the money for a flow hood. And my glove box is soooooo ghetto. Its not even a glove box so much as just a plastic bin with holes in it that i put latex gloves on when I use.


--------------------
..beneath the chaos of the world, all is secretly well..

Rebuilding my DNA.


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Offline4 ho cultivar
Transcending plains
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Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 13 years, 3 months
Re: Pre-Poured Pitri? [Re: Shroominit]
    #9448114 - 12/17/08 03:33 AM (13 years, 5 months ago)

does anyone have a surefire formula for how to make mea???


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:mushroom2::crazy2::mushroom2::crazy2::Patience be thy virtue:crazy2::mushroom2::crazy2::mushroom2:

Blurring the line between reality and dreams
Mr. Winkie says: "Procrastinators unite................................tommorrow!!:ganja:
G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!":cuteshit:


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Invisiblenoobieshroomie
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Posts: 12,769
Loc: Not Too Sure Flag
Re: Pre-Poured Pitri? [Re: Shroominit]
    #9448118 - 12/17/08 03:33 AM (13 years, 5 months ago)

i pour mine in a GB
then wrap with glad wrap(its breathable)
ill post pics of my lions mane cultures for all to see

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
Invisiblenoobieshroomie
Back again
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Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448147 - 12/17/08 03:47 AM (13 years, 5 months ago)

just to show every one im not bull shittin
heres pics of my lions mane cultures







these are my cultures

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
InvisibleShroominit
Part Time Mycologist


Folding@home Statistics
Registered: 11/29/08
Posts: 4,662
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448156 - 12/17/08 03:51 AM (13 years, 5 months ago)

We believed you noobie. Very nice looking culture(s) though. Wish I ordered more dishes. I've got like 1/4lb of agar waiting to be used.

Slacker, that's exactly what my (first) glovebox is. It works well, you just get a mister bottle with clean water (I use store bought distilled) and lightly spray the inside before you work. This will settle everything to the floor. Then since there's very little air movement the box is sterile to work in.


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Invisiblenoobieshroomie
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Loc: Not Too Sure Flag
Re: Pre-Poured Pitri? [Re: Shroominit]
    #9448177 - 12/17/08 03:59 AM (13 years, 5 months ago)

if you want a culture let me know
i dont want actives.would like supplies
new syringes/needles,vials,ect

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
Offline4 ho cultivar
Transcending plains
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Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 13 years, 3 months
Re: Pre-Poured Pitri? [Re: noobieshroomie]
    #9448195 - 12/17/08 04:05 AM (13 years, 5 months ago)

yeah well listen to this: I sprayed my gl.box with IPA{instead of water like suggested.}, then i bathed my arms in IPA{oh yes absolutly wet!}, you know where this is going...... then I stuck my arms in{with the thought just in my head that i need to be carefull and NOT USE A  LIGHTER}. well.......... stuck my arms in, pulled the syringe out and lit the ligher. WHOOSH!!!!

scars are minimal. all hair is gone. my credibility for being safe. gone. I knew not to but I did anyways while not thinking.

don't be like 4 ho. or else youll look like  a girl with no hair on your arms.


--------------------
:mushroom2::crazy2::mushroom2::crazy2::Patience be thy virtue:crazy2::mushroom2::crazy2::mushroom2:

Blurring the line between reality and dreams
Mr. Winkie says: "Procrastinators unite................................tommorrow!!:ganja:
G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!":cuteshit:


Edited by 4 ho cultivar (12/17/08 04:06 AM)


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Invisiblenoobieshroomie
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Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
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Re: Pre-Poured Pitri? [Re: 4 ho cultivar]
    #9448206 - 12/17/08 04:09 AM (13 years, 5 months ago)

Quote:

4 ho cultivar said:
yeah well listen to this: I sprayed my gl.box with IPA{instead of water like suggested.}, then i bathed my arms in IPA{oh yes absolutly wet!}, you know where this is going...... then I stuck my arms in{with the thought just in my head that i need to be carefull and NOT USE A  LIGHTER}. well.......... stuck my arms in, pulled the syringe out and lit the ligher. WHOOSH!!!!

scars are minimal. all hair is gone. my credibility for being safe. gone. I knew not to but I did anyways while not thinking.

don't be like 4 ho. or else youll look like  a girl with no hair on your arms.



fumes+fire in a GB =:onfire:

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Extras: Filter Print Post Top
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