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slacker008
Resident Psychiatrist



Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
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Pre-Poured Pitri?
#9447994 - 12/17/08 02:57 AM (12 years, 2 months ago) |
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I know this sounds silly but is there any place that sells sterile pitri dishes with agar already in them? Maybe I miffed something up but I cant seem to get the agar to NOT contaminate.
-------------------- ..beneath the chaos of the world, all is secretly well..
Rebuilding my DNA.
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pre-Poured Pitri? [Re: slacker008]
#9447997 - 12/17/08 02:58 AM (12 years, 2 months ago) |
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ill hook you up with some
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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wow noobie i love that pic of you with the shrooms.. man i wish i had a clone of that shit!
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slacker008
Resident Psychiatrist



Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
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That'd be a lifesaver. Im wanting to do some cloning work but its hard to do when I cant get agar pouring down.
-------------------- ..beneath the chaos of the world, all is secretly well..
Rebuilding my DNA.
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pre-Poured Pitri? [Re: slacker008]
#9448028 - 12/17/08 03:04 AM (12 years, 2 months ago) |
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thanks trip slacker i can send you some pre poured petries
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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Re: Pre-Poured Pitri? [Re: slacker008]
#9448033 - 12/17/08 03:05 AM (12 years, 2 months ago) |
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maybe you should talk about your method of making it. I have a dish that is about 4 months old never used and it has 0 contams. im waiting to see how long it can stay contam free for.
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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Re: Pre-Poured Pitri? [Re: tripchip]
#9448042 - 12/17/08 03:07 AM (12 years, 2 months ago) |
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send me some too newbie, just dont accidently drop a piece of tissue on it
-------------------- Psilocybe Cubensis
Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba.
CHIP
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noobieshroomie
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Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pre-Poured Pitri? [Re: tripchip]
#9448057 - 12/17/08 03:14 AM (12 years, 2 months ago) |
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trip i can send you a few blanks or one with a lions mane culture your choice
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
Edited by noobieshroomie (12/17/08 03:17 AM)
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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nah im good. unless lions mane is code word..JK ill discover my own fatties that will put yours to shame!
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pre-Poured Pitri? [Re: tripchip]
#9448075 - 12/17/08 03:20 AM (12 years, 2 months ago) |
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Quote:
tripchip said: nah im good. unless lions mane is code word..JK ill discover my own fatties that will put yours to shame!
ha ha ha lions mane is a choice edible it tastes like lobster when cooked right:) PM me
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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LOL its all good dude. im cool. thanks anyways. your a good guy
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Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
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Re: Pre-Poured Pitri? [Re: tripchip]
#9448106 - 12/17/08 03:29 AM (12 years, 2 months ago) |
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Noobie, you're awesome. (so is that pic haha)
Slacker, petri dishes 'breathe' out the sides, so you want to keep them in a sterile area after making them. Also, you want to pour in a glove box or even better a flow hood to keep the contams out.
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slacker008
Resident Psychiatrist



Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
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Re: Pre-Poured Pitri? [Re: Shroominit]
#9448110 - 12/17/08 03:31 AM (12 years, 2 months ago) |
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I dont have the money for a flow hood. And my glove box is soooooo ghetto. Its not even a glove box so much as just a plastic bin with holes in it that i put latex gloves on when I use.
-------------------- ..beneath the chaos of the world, all is secretly well..
Rebuilding my DNA.
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4 ho cultivar
Transcending plains



Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 12 years, 18 days
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Re: Pre-Poured Pitri? [Re: Shroominit]
#9448114 - 12/17/08 03:33 AM (12 years, 2 months ago) |
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does anyone have a surefire formula for how to make mea???
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   :Patience be thy virtue   Blurring the line between reality and dreams
Mr. Winkie says: "Procrastinators unite................................tommorrow!! G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!"
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noobieshroomie
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Registered: 07/19/08
Posts: 12,769
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Re: Pre-Poured Pitri? [Re: Shroominit]
#9448118 - 12/17/08 03:33 AM (12 years, 2 months ago) |
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i pour mine in a GB then wrap with glad wrap(its breathable) ill post pics of my lions mane cultures for all to see
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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just to show every one im not bull shittin heres pics of my lions mane cultures






 these are my cultures
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
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We believed you noobie. Very nice looking culture(s) though. Wish I ordered more dishes. I've got like 1/4lb of agar waiting to be used.
Slacker, that's exactly what my (first) glovebox is. It works well, you just get a mister bottle with clean water (I use store bought distilled) and lightly spray the inside before you work. This will settle everything to the floor. Then since there's very little air movement the box is sterile to work in.
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pre-Poured Pitri? [Re: Shroominit]
#9448177 - 12/17/08 03:59 AM (12 years, 2 months ago) |
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if you want a culture let me know i dont want actives.would like supplies new syringes/needles,vials,ect
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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4 ho cultivar
Transcending plains



Registered: 11/18/08
Posts: 379
Loc: Central, Oregon {represen...
Last seen: 12 years, 18 days
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yeah well listen to this: I sprayed my gl.box with IPA{instead of water like suggested.}, then i bathed my arms in IPA{oh yes absolutly wet!}, you know where this is going...... then I stuck my arms in{with the thought just in my head that i need to be carefull and NOT USE A LIGHTER}. well.......... stuck my arms in, pulled the syringe out and lit the ligher. WHOOSH!!!!
scars are minimal. all hair is gone. my credibility for being safe. gone. I knew not to but I did anyways while not thinking.
don't be like 4 ho. or else youll look like a girl with no hair on your arms.
--------------------
   :Patience be thy virtue   Blurring the line between reality and dreams
Mr. Winkie says: "Procrastinators unite................................tommorrow!! G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!"
Edited by 4 ho cultivar (12/17/08 04:06 AM)
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noobieshroomie
Back again




Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
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Quote:
4 ho cultivar said: yeah well listen to this: I sprayed my gl.box with IPA{instead of water like suggested.}, then i bathed my arms in IPA{oh yes absolutly wet!}, you know where this is going...... then I stuck my arms in{with the thought just in my head that i need to be carefull and NOT USE A LIGHTER}. well.......... stuck my arms in, pulled the syringe out and lit the ligher. WHOOSH!!!!
scars are minimal. all hair is gone. my credibility for being safe. gone. I knew not to but I did anyways while not thinking.
don't be like 4 ho. or else youll look like a girl with no hair on your arms.
fumes+fire in a GB =
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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