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InvisibleBEEP
Registered: 01/06/08
Posts: 1,385
Agar contamd?
    #9433334 - 12/14/08 10:11 PM (15 years, 2 months ago)

I placed a tissue sample of a fruit on some MEAgar and it got contamd with trich here and there, so i moved the myc over to a new dish, its been about 2 weeks and no trich, thats good :smile: but i noticed something sorta weird on it i think might be bacteria, (there's some pictures bellow). i haven't found alot of pictures of agar plates so i have no idea if its normal, i know this much myc for 2 weeks sucks, I've been incubating at 65f :lol: i don't want to bother with heating, or maybe the strain is a slow colonizer or the plate is contaminated :frown: heres the pics. sadly this is also my first shot at agar and Ive been cultivating for about a year now :grin:







you think its bacteria? it doesn't smell like anything weird so it kinda threw me off.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Agar contamd? [Re: BEEP]
    #9433382 - 12/14/08 10:18 PM (15 years, 2 months ago)

You're right.  The milky slime is bacteria.  It looks like there's also some mushroom mycelium in there too, so try taking some tiny pieces of the mushroom mycelium no larger than a grain of rice and move them to new dishes. 

As soon as it starts to grow, grab a piece of the fresh growth and repeat the process.  Continue until you get a clean culture.  It usually takes three or four transfers to get completely away from the contaminant.  Antibiotic agar will help if you have it.
RR


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Offlinesolumvita
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Registered: 02/12/08
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Re: Agar contamd? [Re: RogerRabbit]
    #9437131 - 12/15/08 02:24 PM (15 years, 2 months ago)

the top right of the dish looks like you may have an area less full of bacteria.  I would start there.


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InvisibleBEEP
Registered: 01/06/08
Posts: 1,385
Re: Agar contamd? [Re: solumvita]
    #9443079 - 12/16/08 11:07 AM (15 years, 2 months ago)

nasty stuff, i wonder why it doesn't smell, thanks for the help guys i'm going to do some transfers today.


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OfflineRogerRabbitM
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Re: Agar contamd? [Re: BEEP]
    #9443108 - 12/16/08 11:13 AM (15 years, 2 months ago)

Petri dishes rarely smell because there's not enough there.  Now, a jar of contaminated grains is a different story, since there's much more bacteria.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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