|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
BEEP
Registered: 01/06/08
Posts: 1,385
|
Agar contamd?
#9433334 - 12/14/08 10:11 PM (15 years, 2 months ago) |
|
|
I placed a tissue sample of a fruit on some MEAgar and it got contamd with trich here and there, so i moved the myc over to a new dish, its been about 2 weeks and no trich, thats good but i noticed something sorta weird on it i think might be bacteria, (there's some pictures bellow). i haven't found alot of pictures of agar plates so i have no idea if its normal, i know this much myc for 2 weeks sucks, I've been incubating at 65f i don't want to bother with heating, or maybe the strain is a slow colonizer or the plate is contaminated heres the pics. sadly this is also my first shot at agar and Ive been cultivating for about a year now 



you think its bacteria? it doesn't smell like anything weird so it kinda threw me off.
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
|
Re: Agar contamd? [Re: BEEP]
#9433382 - 12/14/08 10:18 PM (15 years, 2 months ago) |
|
|
You're right. The milky slime is bacteria. It looks like there's also some mushroom mycelium in there too, so try taking some tiny pieces of the mushroom mycelium no larger than a grain of rice and move them to new dishes.
As soon as it starts to grow, grab a piece of the fresh growth and repeat the process. Continue until you get a clean culture. It usually takes three or four transfers to get completely away from the contaminant. Antibiotic agar will help if you have it. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
solumvita
Q.B.E.


Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 8 months, 6 days
|
|
the top right of the dish looks like you may have an area less full of bacteria. I would start there.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
|
BEEP
Registered: 01/06/08
Posts: 1,385
|
Re: Agar contamd? [Re: solumvita]
#9443079 - 12/16/08 11:07 AM (15 years, 2 months ago) |
|
|
nasty stuff, i wonder why it doesn't smell, thanks for the help guys i'm going to do some transfers today.
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
|
Re: Agar contamd? [Re: BEEP]
#9443108 - 12/16/08 11:13 AM (15 years, 2 months ago) |
|
|
Petri dishes rarely smell because there's not enough there. Now, a jar of contaminated grains is a different story, since there's much more bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,289 topic views. 29 members, 146 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|