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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: veda_sticks]
#10476579 - 06/09/09 11:13 AM (14 years, 9 months ago) |
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Just a note, when printing on foil after the spores have dropped and you removed the cap from the foil leave the print in the GB so that the spores can dry abit. If you don't when you close it will smudge and it will look awful.
Heres a pic; left B+ that I made, centre Mex Alb. (apprently from someone who got it from a TC! who then gave it to me) and right a print I got in a trade which it unused...shocking. What were they thinking?
I don't mean to moan but some peoples prints are of very poor quality
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durantz
Stranger
Registered: 05/09/09
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#10478330 - 06/09/09 05:18 PM (14 years, 9 months ago) |
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ok so I was thinking what if I got some foil and a ziplock bag inside a glovebox. Then I could wash both the bag and the foil with chlorhexidine or methylated spirits. Turn the bag inside out and let it and the foil dry. Then once it is dry I could place the foil inside the bag making sure it is flat and zip the bag up.
When I come across a wild mushroom I flick the lighter out and heat my knife up then take the stem off. I quickly open the ziplock bag and place the cap on the foil and let it sit for the required time. Then just remove the cap and seal the bag up again.
Any comments?
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#10478510 - 06/09/09 05:53 PM (14 years, 9 months ago) |
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Quote:
badman said: Just a note, when printing on foil after the spores have dropped and you removed the cap from the foil leave the print in the GB so that the spores can dry abit. If you don't when you close it will smudge and it will look awful.
Heres a pic; left B+ that I made, centre Mex Alb. (apprently from someone who got it from a TC! who then gave it to me) and right a print I got in a trade which it unused...shocking. What were they thinking?
I don't mean to moan but some peoples prints are of very poor quality
Thats a common problem with open face prints, they dont stand up to well to the mail. It doesnt look to bad actually. somce ot was on paper though its stuck quite well.
No idea waht happened to the 3rd print, ive never receied a print like that.
Definatly allow your prints to dry properly. I recieved a print was folded up still moist, the print was like if it were baked on to the foil, and when i moistened it to get the spores off, it came of in flakes. Ive been shaking the spore jar every day for the past week and the clmps still dont break up.
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10478844 - 06/09/09 06:55 PM (14 years, 9 months ago) |
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Quote:
durantz said: ok so I was thinking what if I got some foil and a ziplock bag inside a glovebox. Then I could wash both the bag and the foil with chlorhexidine or methylated spirits. Turn the bag inside out and let it and the foil dry. Then once it is dry I could place the foil inside the bag making sure it is flat and zip the bag up.
When I come across a wild mushroom I flick the lighter out and heat my knife up then take the stem off. I quickly open the ziplock bag and place the cap on the foil and let it sit for the required time. Then just remove the cap and seal the bag up again.
Any comments?
Personally, but i haven't gone shroom picking, i'd wait till i got home to print them decently.
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durantz
Stranger
Registered: 05/09/09
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#10479289 - 06/09/09 08:32 PM (14 years, 9 months ago) |
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Problem is that I'm going to be out in the field for about 2 months so I will have no home to go back to... was wondering if my above mentioned method would be the best way.
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10481201 - 06/10/09 06:09 AM (14 years, 9 months ago) |
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will you be setting up tents and camping in one spot for 24 or more hours?
you cant walk about with the print on you or in your bag or whatever it needs to be left undisturbed.
the way you wanna do it youll get a smudged print, make a 'book' like it the pic then bag it when dry.
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durantz
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#10484197 - 06/10/09 06:47 PM (14 years, 9 months ago) |
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the most I'll be in one spot for is about 14 hours when I set up camp for the night. I was going to do the spore printing then.
I just wanted to know the best possible way under these conditions.
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Mogombus
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Re: Another Way To Take a Sterile Spore Print? [Re: veda_sticks]
#10548629 - 06/21/09 04:26 PM (14 years, 8 months ago) |
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Amazing. I need to make spore prints and eventually my own syringes.
I live in a state where I cannot get ready-made delivered in the mail. Originally, I brought some ready-made store bought syringes from out of state for my on-going project, which I'm going to wind down for a while. I will want to re-start after an indefinite period of time and keeping spore prints is my only option.
Now after reading here I understand that just plopping a cap onto a foil square right out of the box, letting is sit in an open room for a day or two is not good enough. Since I really want to have success from my own spores.
Any other suggestions for a first timer spore saver like me?
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heather95966
Stranger
Registered: 09/20/09
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Last seen: 14 years, 4 months
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Does anyone know were I might be able to get a print? I kinda was hopeing to get one for free being this will be my first try and I don't wan't to spend alot of money. If I have a successful yeild I will pass on the favor. I know this might be far fetched but I would really appreciate it.
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: heather95966]
#11096188 - 09/20/09 06:00 PM (14 years, 5 months ago) |
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Quote:
heather95966 said: Does anyone know were I might be able to get a print? I kinda was hopeing to get one for free being this will be my first try and I don't wan't to spend alot of money. If I have a successful yeild I will pass on the favor. I know this might be far fetched but I would really appreciate it.
Pm me
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gardengirl
student of growth
Registered: 08/31/09
Posts: 15
Loc: the desert
Last seen: 3 years, 28 days
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Re: Another Way To Take a Sterile Spore Print? [Re: veda_sticks]
#11103502 - 09/21/09 10:09 PM (14 years, 5 months ago) |
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So Veda, would you suggest if one has a print that is (probably) borderline sterile, and wants to use the small container method, it is better to add 20-30 ccs of sterilized water instead of just 10? Let's say the print is on the light side, just a 24 hour one that doesn't look very heavy with spores. If there is a benefit to diluting it (extra contam. spores avoidance) then it seems advisable even with a lighter print. What is your educated opinion?
-------------------- "Knowledge is the only fountain both of love and the principles of human liberty." -Daniel Webster, 1843
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feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#11103599 - 09/21/09 10:27 PM (14 years, 5 months ago) |
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Quote:
badman said:
Quote:
heather95966 said: Does anyone know were I might be able to get a print? I kinda was hopeing to get one for free being this will be my first try and I don't wan't to spend alot of money. If I have a successful yeild I will pass on the favor. I know this might be far fetched but I would really appreciate it.
Pm me
agreed always ready to help a girl just did these today
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: feelfunny]
#11105445 - 09/22/09 08:56 AM (14 years, 5 months ago) |
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I never got a PM, I only have two spare cube prints left. Never mind.
To gardengirl, sterility is a black and white issue there is no grey area.
Its sterile or not.
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gardengirl
student of growth
Registered: 08/31/09
Posts: 15
Loc: the desert
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#11110790 - 09/23/09 03:14 AM (14 years, 5 months ago) |
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Badman, maybe I was being unclear...it was my understanding that truly sterile prints are not really achievable even with cultivated mushrooms. (I might be wrong on this.)
The scenario I presented assumes that all reasonable and customary procedures are being followed to obtain a "sterile" print from cultivated fungi. I do understand why you would want to make sure someone new to the forum realizes that either it's clean or it's contaminated. I was curious about Veda's post because I did not consider the possibility that too many spores added to injection solution could give any unwanted microbes an advantage in reproduction in the substrate. (If there were a few in the print, there would be more in a dense solution.) Do you see what I was asking? Sorry if I am missing something here.
-------------------- "Knowledge is the only fountain both of love and the principles of human liberty." -Daniel Webster, 1843
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: gardengirl]
#11110851 - 09/23/09 03:49 AM (14 years, 5 months ago) |
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You could get very very clean prints but not sterile. I dont think you could get a sterile print unless your whole procedure is sterile, I mean absolutely everything down to the room the fc is in.
We normally use prints which are clean enough to use directly as the inoculant however, any syringe you make has the possibility of contaminating and obviously the risk increases with poor printing method.
You are correct about more spores increases risk of contamming always use the smallest possible amount of spores when doing anything.
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ENtiT
Stranger
Registered: 11/02/11
Posts: 42
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Re: Another Way To Take a Sterile Spore Print? [Re: badman]
#15363020 - 11/13/11 01:08 AM (12 years, 4 months ago) |
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I know this is an old thread but a solution re the collection of sterile prints from wild collections is not to collect spore at all. Rather make some peroxide agar slants to take along camping and clone the fruits. Collect fruit samples that you wish to propegate and store them in paper bags until you get back to camp. When in camp in a place where there is still air (tent), remove stem, brush any loose dirt and debis free from the cap, flame sterilize your tweezers, crack the cap of the shroom in half and dig out a sample from the center of the cap where it is sterile, dunk in a dilute peroxide solution then drop into agar slant. There you go, sterile clone that you can grow on and make sterile prints from.
You could go to greater lengths to ensure sterility of the sample such as using gloves and cleaning the cap with alcohol prior to cracking the cap open but it all depends upon how much gear you want to carry. The dunk in peroxide solution pretty well covers airborn contam issues between taking sample and transfer to slant and the agar contains peroxide anyway so its probably not needed.
Have I tried this method? Not in the field. I have cloned oyster fruits from the supermarket this way as well as an edible field mushroom found in a local park. Open air, no glove box It does work
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CH HELL
Brain Sturgeon
Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Another Way To Take a Sterile Spore Print? [Re: ENtiT]
#15372387 - 11/15/11 12:19 AM (12 years, 4 months ago) |
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Wow, A two year old thread that you would like to spread mis information on.
There is NO easier way to get a clean culture than getting spores.
If you are truly in the "field" then you would know that it is best to take a print. There are several was to do it. Place your caps in a zip lock bag and make prints later, or place cap in a paper bag and then a zip lock. But whatever you do you want to get spores, pictures and as much info as you can if you are gathering more than one type of mushroom. CH
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San
Novice
Registered: 10/30/11
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Re: Another Way To Take a Sterile Spore Print? [Re: CH HELL]
#15372422 - 11/15/11 12:28 AM (12 years, 4 months ago) |
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Just do agar.
Geez. If you've got a pressure cooker it's so much cleaner.
--------------------
Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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cdmc1984
ilikeurmoms
Registered: 10/01/11
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#15437258 - 11/28/11 07:52 PM (12 years, 3 months ago) |
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OK I think I got you on this one any one feel free to help out on this.
list presterilized latex gloves alcohol spray bottle sorry i put wipes in down there\/ envelopes as many as needed zip-lock bags as many as needed
OK presterilize your gloves with alcohol then place them in a zip lock bag, do the same with your envelopes. Sterilizing each one and placing it in its own bag pack everything in one more bag and head out. When you find the mushroom you wish to print take out your gloves pick and put the mushroom rite into your envelope. Seal and put it back in the bag if your going to be out for to long you will need desiccant/absorbent. They come in all kinds of stuff shoes med pack it's like a salt pack. BEWARE salt kills fungi desiccant is not salt I think not sure, you should put the desiccant in the envelope. It should be placed outside the envelope but in the bag seal well by hand, then seal it with a lighter. Instead of the wipes I would take a small spray bottle about 2 or 3 fl oz for your gloves to keep from cross contamination.
now for my question i need to sterilize water for spore print and all i have is fish tank air hose no PC no MASON jars just plastic bottles alcohol and peroxide i got a wine bottle basically i need to sterilize water with stuff from my trash can i got a bertolli jar with one peice lid i been holding on to but thats about it please help i got all these print and i keep runing in to the green meanie /other wise know as green mold
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kansastornado
Stranger
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#15439586 - 11/29/11 09:59 AM (12 years, 3 months ago) |
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Quote:
durantz said: ok so I was thinking what if I got some foil and a ziplock bag inside a glovebox. Then I could wash both the bag and the foil with chlorhexidine or methylated spirits. Turn the bag inside out and let it and the foil dry. Then once it is dry I could place the foil inside the bag making sure it is flat and zip the bag up.
When I come across a wild mushroom I flick the lighter out and heat my knife up then take the stem off. I quickly open the ziplock bag and place the cap on the foil and let it sit for the required time. Then just remove the cap and seal the bag up again.
Any comments?
The way I do it is take your mushroom head and stick it in a mason jar and tighten the lid. Then stick the jar inside a small sterlite tote and put in cool dark place. When spores drop, remove the mushroom head and fill the jar up with a fresh unopened distilled water jug and then seal tight. Put jar or jars into 1-2 brown paper bags so light will not shine through and stick in the refrigerator. When ready to use I get my glove box out, and heat my flat head screw driver up until red hot.(heat the whole part of the screw driver that is going into the solution, not just the tip) I open the spore jar and scrape the heck out of the spore print to mix with the water. You never have to hydrate them, because they are always hydrated. I never put a hole in lid for the syringe. When ready for use, I put them in glove box and heat needle red hot and just open the jar and fill the syringe.I have had 100% success rate with this even after oppening the jar many many times to fill different syringes. I kept one such solution for 5 years just as an experiment and it colonized my jar in 2 weeks. Its worth a shot. Always wipe down your jar with an alcohol soaked rag when first using and when you are done and ready to put up until next time.
Edited by kansastornado (11/29/11 01:39 PM)
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