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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Another Way To Take a Sterile Spore Print? 1
#9430409 - 12/14/08 01:48 PM (15 years, 3 months ago) |
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Greetings, I was wondering, does anyone have a full proof way of making a sterile spore print? I've read several previous posts on this topic, but the technique always involves having the mushroom cap sit directly on the surface that collects the spores. Touching the mushroom cap to the collection surface seems like a good way to transfer contamination. I was wondering, has anyone ever tried suspending the mushroom cap just above the collection paper with a wire platform in the shape of a apple slicer? While the mushroom cap is shedding its spores, the exterior surface of the mushroom cap could also be further cleaned by having it exposed to an ultraviolet germicidal lamp.
Has anyone tried this technique before? Comments anyone?
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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Elixier
Awesome
Registered: 11/16/08
Posts: 287
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Re: Another Way To Take a Sterile Spore Print? [Re: AdoreChampignons]
#9430424 - 12/14/08 01:51 PM (15 years, 3 months ago) |
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Aluminum foil is made under extreme temperatures so after about the first foot it is "sterile". I would say suspending it would allow air to come up under the cap and contaminate it.
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Re: Another Way To Take a Sterile Spore Print? [Re: Elixier]
#9430468 - 12/14/08 02:00 PM (15 years, 3 months ago) |
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Quote:
Elixier said: Aluminum foil is made under extreme temperatures so after about the first foot it is "sterile". I would say suspending it would allow air to come up under the cap and contaminate it.
Thanks for your comments. I'll keep that in mind. I think I'll try a variety of ways and see which ones seem to curb contaminants the best.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 6 months
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Re: Another Way To Take a Sterile Spore Print? [Re: AdoreChampignons]
#9430497 - 12/14/08 02:07 PM (15 years, 3 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/7335688#7335688 Guaranteed sterile spore print/syringe - Mushroom Cultivation - Shroomery Message Board
http://www.shroomery.org/forums/showflat.php/Number/5862129#5862129 Possible to get a contam free spore print from a shroom in a field? - Mushroom Cultivation - Shroomery Message Board
http://www.shroomery.org/forums/showflat.php/Number/8968322#8968322 Contam then print? - Contamination Forum - Shroomery Message Board
http://www.shroomery.org/forums/showflat.php/Number/2253012#2253012 spore print idea, need input - Mushroom Cultivation - Shroomery Message Board
Yeah, it's been done....here's some links I like. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 6 months
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Re: Another Way To Take a Sterile Spore Print? [Re: Psuper]
#9430500 - 12/14/08 02:08 PM (15 years, 3 months ago) |
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So, one, you can suspend the cap. Two, you can print the cap a second time, after a couple hours, to obtain a cleaner print. Third, you can do both. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Another Way To Take a Sterile Spore Print? [Re: Psuper]
#9430529 - 12/14/08 02:16 PM (15 years, 3 months ago) |
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I found gold in one of the posts here that said:
Make a hole in the top of a jar lid and seal it with silicone.
Now take a mushroom, remove the stem, and put a pin through it. Poke through the bottom and out the top.
Now stick the pin in the silicone and put the lid on.
Now simply remove the lid after the print is done.
Amazing, huh?
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HoneyComber
Stranger
Registered: 08/25/08
Posts: 266
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Re: Another Way To Take a Sterile Spore Print? [Re: Psuper]
#9430541 - 12/14/08 02:19 PM (15 years, 3 months ago) |
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i was thinking........ pick the mushroom with a plastic baggie (the way people pick up their dogs poo) close the bag up bring it to your glove box flame sterilize an xacto blade to cut the cap off print it on some clean foil in your glove box...maybe even bake the foil for 20min at high heat before using it fold it, tag it, bag it.........in your glove box
-------------------- "we're all mad here"
Edited by HoneyComber (12/14/08 02:20 PM)
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Re: Another Way To Take a Sterile Spore Print? [Re: Psuper]
#9430578 - 12/14/08 02:26 PM (15 years, 3 months ago) |
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Quote:
Psuper said: So, one, you can suspend the cap. Two, you can print the cap a second time, after a couple hours, to obtain a cleaner print. Third, you can do both. ~Pixie~
Wow! Thanks for looking those all up for me. I can clearly see why people take two spore prints from the same mushroom but prefer use the second one.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Another Way To Take a Sterile Spore Print? [Re: HoneyComber]
#9430579 - 12/14/08 02:26 PM (15 years, 3 months ago) |
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Seems like it would be a lot of work comber.
The spores themselves are gonna be sterile, so if you have a sterile jar and not too much air movement (sealed jars have still air), then the method I posted would be very clean.
Your contams will almost all come from: carelessness or air movement carrying in contams.
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Re: Another Way To Take a Sterile Spore Print? [Re: HoneyComber]
#9430623 - 12/14/08 02:33 PM (15 years, 3 months ago) |
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Quote:
HoneyComber said:...pick the mushroom with a plastic baggie (the way people pick up their dogs poo) close the bag up bring it to your glove box...
Thank you very much HoneyComber for your comments. That's a very good way to reduce contaminants.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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HoneyComber
Stranger
Registered: 08/25/08
Posts: 266
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Re: Another Way To Take a Sterile Spore Print? [Re: Shroominit]
#9430666 - 12/14/08 02:39 PM (15 years, 3 months ago) |
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i guess baking the foil might be overkill, but the rest seems pretty easy...... to me i like to make lots of prints too, but i dont have lots of jars. foil is abundant in the house, and it takes less space to store prints on folded foil, and they're easier to mail away. not knocking your method at all, jars are good to print in cuz you can just add water and make a syringe...and like you said, in a sealed jar is still air
thank you Adore, now i feel special
-------------------- "we're all mad here"
Edited by HoneyComber (12/14/08 02:42 PM)
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HybridprX
Biodegrader of coir
Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 6 years, 6 months
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Re: Another Way To Take a Sterile Spore Print? [Re: Shroominit]
#9430720 - 12/14/08 02:48 PM (15 years, 3 months ago) |
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I normally cutt out the tin foil squares, stack them and wrap in foil then sterilize them at 15psi for 20minutes along with the spore syringes which I also wrap in foil, after everything has cooled down I transfer them to a pre-cleaned glovebox with a few rubber maid containers wiped clean with bleach prior to.
You can even PC the rubber maids if they're (I'm pretty sure) PP2 or is it pp6 type plastics...I'll check for you in abit, but you could just wrap them in tinfoil and PC along with everything else if your pressure cooker is large enough.
Anyways, if you take each square and line the containers with them then use a very fine mist of H2o2 to mist the tinfoil squares and place on the cap using a piece of wire like in the tek psuper posted or thumb tack, even a nail will work....theory of mine was that the initial spores would drop with any contaminate spores that were fortunate enough to land on the inner gills, since peroxide evaporates fairly quickly by the time the contaminate spores have dropped they're killed off then the peroxide slowly evaporates and leaves you with a nice cubensis print that is always 100% sterile, I've done my prints this way even in open air for years and have only ever encountered contamination when I either dropped the container with the caps in it or did not properly seal the spore print baggy prior to storage. As for the technique itself its pretty sound proof If I don't mind saying so myself :p
A much easier way is the jar method for sure, but even those I would either put the tinfoil square in and put the lid on and PC with the syringes prior to using them or just the jar...
Here's a pic of some prints I made up the way I described to you...they're Pink Buffalo, I started it back up after four years of taking the original print using the peroxide method without the use of a glovebox (use a glovebox if you have one)
Now days I just sterilize the water in the syringes by wrapping it up in foil and placing it plunger side down and packing for 20 minutes, take one of those bagged prints into the glovebox after the syringe is cooled, wipe off one side close to the print, take a marker and put a little dab where the hole with be and inject 2cc's of water, use my finger to rub the water against the spores on the foil and suck it back up into the syringe and repeat 20x...
They're also handy for trades.
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Re: Another Way To Take a Sterile Spore Print? [Re: HybridprX]
#9430798 - 12/14/08 03:02 PM (15 years, 3 months ago) |
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Quote:
HybridprX said:...I normally cutt out the tin foil squares, stack them and wrap in foil then sterilize them at 15psi for 20minutes along with the spore syringes which I also wrap in foil, after everything has cooled down I transfer them to a pre-cleaned glovebox with a few rubber maid containers wiped clean with bleach prior to...
Ah, you use tin foil also. Have you tried using sheets of tyvek instead of tin foil? I find that it's easier to squirt some sterile water from a syringe onto a spore print taken on tyvek than on tin foil spore print. The tin foil tends to get punctured a little too easy.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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durantz
Stranger
Registered: 05/09/09
Posts: 697
Last seen: 9 years, 2 months
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hey what I'd like to know is how to take a sterile spore print while you're in the field...
I'm going to be going on an extended hiking trip and I won't really have the chance to take anything to a sterile location.
Can anyone think of a way for me to get a few sterile spore prints? Any equipment has to be light and compact. Seriously as small as possible.
Also just wondering if it is actually possible to sterilise a contaminated spore print...
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10469983 - 06/08/09 09:37 AM (14 years, 9 months ago) |
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It almost impossible to make sterile spore prints from the wild but... Use a sterile plastic tub and sterile paper towels, loosely wrap the shrooms in the paper and place in the box. Print when you get indoors spray the outside box with alcohol and make them in a GB.
contamed print is again too difficult to sterilise youll kill spores most likely.
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CH HELL
Brain Sturgeon
Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10469987 - 06/08/09 09:39 AM (14 years, 9 months ago) |
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It is not possible to get a sterile print from a wild shroom. But you can wrap the cap in foil or place it in a petri dish while in the feild to get your print. CH
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durantz
Stranger
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Re: Another Way To Take a Sterile Spore Print? [Re: CH HELL]
#10472345 - 06/08/09 05:30 PM (14 years, 9 months ago) |
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Alright so not possible to sterilise a spore print after you've taken it.
So the solution would be to grow the contaminated spore print on agar then monitor it closely for any contaminations. Then quickly isolate a clean piece of mycelium and transfer to sterile grain?
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Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 6 months
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10472379 - 06/08/09 05:36 PM (14 years, 9 months ago) |
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Quote:
durantz said: Alright so not possible to sterilise a spore print after you've taken it.
So the solution would be to grow the contaminated spore print on agar then monitor it closely for any contaminations. Then quickly isolate a clean piece of mycelium and transfer to sterile grain?
When transfering away from contams you take the outermost edge of rhizomorphic mycelium and transfer that to a clean dish- that clean dish is then used to inoculate grains. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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durantz
Stranger
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Re: Another Way To Take a Sterile Spore Print? [Re: Psuper]
#10472457 - 06/08/09 05:52 PM (14 years, 9 months ago) |
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Cool thanks for that. It sounds like I've got a lot of work and research ahead of me. It's pretty exciting I must say.
Can't wait to go out and get some wild spore prints.
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veda_sticks
Cultivator
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Loc: UK
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Re: Another Way To Take a Sterile Spore Print? [Re: durantz]
#10473267 - 06/08/09 08:11 PM (14 years, 9 months ago) |
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even an indoor grown mushroom print isnt sterile, no matter how clean you are with your work area. Fruits are exposed to unsterile conditions.
Spore prints will more than likely contain a small amount of contaminants.
A few of those printing methods are old and outdated. The 1 that uses a jar mentions to inject 10cc water nto the jar then draw back up, which would create a very dark syringe. Dark syringes are counter productive. Apart from that its a nice tek.
ive recieved quite a few prints that were on foil and they have all worked great. And im sure they were made using pretty standard method of transfering the cap to a peice of foil inside a glovebox and allowed to drop for 24 hours.
If you make up a spore syringe with too many spores, thats when things can go wrong, not only will too many spores hurt performance, but also pick up more contaminant spores from a print.
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